LOCUS       Exported                9780 bp ds-DNA    circular SYN 20-MAR-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pTet-O-NEUROG1-T2A-PuroR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   JoshBlack099
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2..55
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /protein_id="
                     "
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             62..661
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    700..1288
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(1171..1182)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(1291..1307)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             1813..1993
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2025..2249
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2295..2723
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2737..3066
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2917..3052
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        3114..3161
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             3180..3554
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3684..3805
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3854..3870)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3878..3894
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3902..3932)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3947..3968
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4256..4844)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5015..5875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5876..5980)
                     /gene="bla"
                     /note="AmpR promoter"
     enhancer        6246..6625
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6627..6825
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6843..7023
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7070..7195
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7688..7921
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8448..8565
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8619..8889
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        8922..8959
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     gene            9065..9775
                     /note="NEUROG1"
ORIGIN
        1 agagggcagg ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gcccactcga
       61 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
      121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
      181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
      241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
      301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
      361 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
      421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
      481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
      541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
      601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
      661 attaattaag ctagctaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
      721 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      781 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      841 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      901 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      961 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     1021 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     1081 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     1141 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     1201 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     1261 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     1321 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     1381 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     1441 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     1501 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     1561 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     1621 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     1681 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     1741 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     1801 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     1861 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1921 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1981 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     2041 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     2101 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     2161 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     2221 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     2281 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     2341 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     2401 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2461 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     2521 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2581 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     2641 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2701 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     2761 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2821 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2881 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2941 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3001 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3061 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     3121 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     3181 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     3241 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     3301 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     3361 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     3421 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     3481 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     3541 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     3601 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3661 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     3721 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3781 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     3841 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3901 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3961 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4021 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4081 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4141 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4681 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4861 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4921 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4981 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5041 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5101 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5161 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5221 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5281 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5341 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5401 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5461 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5521 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5581 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5641 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5701 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5761 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5821 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5881 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5941 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6001 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     6061 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     6121 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     6181 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     6241 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     6301 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     6361 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     6421 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     6481 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     6541 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     6601 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     6661 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     6721 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     6781 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
     6841 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6901 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6961 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7021 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     7081 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     7141 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     7201 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     7261 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     7321 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     7381 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7441 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7501 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     7561 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     7621 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7681 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7741 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     7801 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7861 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7921 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7981 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     8041 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     8101 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     8161 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     8221 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     8281 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     8341 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     8401 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     8461 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     8521 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     8581 gacagcagag atccagtttg gttactcgag tttaccactc cctatcagtg atagagaaaa
     8641 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     8701 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     8761 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     8821 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     8881 tgatagagaa aagtgaaagt cgagctcggt acccgggtcg agtaggcgtg tacggtggga
     8941 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     9001 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaattcgc
     9061 caccatgcca gcccgccttg agacctgcat ctccgacctc gactgcgcca gcagcagcgg
     9121 cagtgaccta tccggcttcc tcaccgacga ggaagactgt gccagactcc aacaggcagc
     9181 ctccgcttcg gggccgcccg cgccggcccg caggggcgcg cccaatatct cccgggcgtc
     9241 tgaggttcca ggggcacagg acgacgagca ggagaggcgg cggcgccgcg gccggacgcg
     9301 ggtccgctcc gaggcgctgc tgcactcgct gcgcaggagc cggcgcgtca aggccaacga
     9361 tcgcgagcgc aaccgcatgc acaacttgaa cgcggccctg gacgcactgc gcagcgtgct
     9421 gccctcgttc cccgacgaca ccaagctcac caaaatcgag acgctgcgct tcgcctacaa
     9481 ctacatctgg gctctggccg agacactgcg cctggcggat caagggctgc ccggaggcgg
     9541 tgcccgggag cgcctcctgc cgccgcagtg cgtcccctgc ctgcccggtc ccccaagccc
     9601 cgccagcgac gcggagtcct ggggctcagg tgccgccgcc gcctccccgc tctctgaccc
     9661 cagtagccca gccgcctccg aagacttcac ctaccgcccc ggcgaccctg ttttctcctt
     9721 cccaagcctg cccaaagact tgctccacac aacgccctgt ttcattcctt accactctag
//
