LOCUS       Exported                3898 bp ds-DNA     circular SYN 10-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPEG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Bogdanove Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 20, 2020 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..16
                     /note="fwd primers"
     misc_feature    1
                     /note="Not Defined"
     misc_feature    1
                     /note="Not Defined"
     misc_recomb     33..132
                     /note="attL1"
     promoter        157..614
                     /label=CmYLCV
     tRNA            631..707
                     /label=tRNA (Gly)
     misc_RNA        731..806
                     /label=g RNA scaffold2
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     tRNA            807..883
                     /label=tRNA (Gly)
     terminator      894..1143
                     /label=HSP terminator
     misc_feature    1148..1165
                     /label=Gibson overlap1
     misc_feature    1194..1210
                     /label=Gibson overlap2
     misc_recomb     1241..1340
                     /note="attL2"
     promoter        1475..1608
                     /label=Spn prom
                     /note="Spn\promoter"
     CDS             1609..2619
                     /note="SpnR"
     rep_origin      2686..3359
                     /note="ori"
     terminator      3630..3657
                     /label=rrnB\T2\transcription\terminat
                     /note="rrnB\T2\transcription\terminat"
     terminator      3789..3832
                     /label=T1-T2
                     /note="T1-T2"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccggaatt cacctgccag gggacctggc agacatactg tcccacaaat
      181 gaagatggaa tctgtaaaag aaaacgcgtg aaataatgcg tctgacaaag gttaggtcgg
      241 ctgcctttaa tcaataccaa agtggtccct accacgatgg aaaaactgtg cagtcggttt
      301 ggctttttct gacgaacaaa taagattcgt ggccgacagg tgggggtcca ccatgtgaag
      361 gcatcttcag actccaataa tggagcaatg acgtaagggc ttacgaaata agtaagggta
      421 gtttgggaaa tgtccactca cccgtcagtc tataaatact tagcccctcc ctcattgtta
      481 agggagcaaa atctcagaga gatagtccta gagagagaaa gagagcaagt agcctagaag
      541 tagtcaaggc ggcgaagtat tcaggcacgt ggccaggaag aagaaaagcc aagacgacga
      601 aaacaggtaa gagctaagct tcgcatgcag aacaaagcac cagtggtcta gtggtagaat
      661 agtaccctgc cacggtacag acccgggttc gattcccggc tggtgcaaga gaccaagacg
      721 tcaggtctca gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      781 ttgaaaaagt ggcaccgagt cggtgcaaca aagcaccagt ggtctagtgg tagaatagta
      841 ccctgccacg gtacagaccc gggttcgatt cccggctggt gcagtcgatc aagatatgaa
      901 gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt
      961 tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat
     1021 cacattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc
     1081 ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat
     1141 aagaactcgc gcgtagtcct cggtaatatc gcagaacaaa agtacctgat atcgagtgta
     1201 cttcaagtca caccggcgag tgactagcag gtggaattcg acccagcttt cttgtacaaa
     1261 gttggcatta taaaaaataa ttgctcatca atttgttgca acgaacaggt cactatcagt
     1321 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1381 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1441 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     1501 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     1561 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     1621 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     1681 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1741 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1801 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     1861 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1921 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     1981 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2041 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2101 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2161 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2221 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2281 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2341 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2401 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2461 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2521 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     2581 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     2641 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2821 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3061 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     3121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3361 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3421 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3481 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3541 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3601 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3661 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3721 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3781 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3841 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgtt
//
