LOCUS       Exported               18478 bp ds-DNA     circular SYN 23-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPPEM
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 18478)
  AUTHORS   Bogdanove Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 2, 2020 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18478
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(58..88)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        315..992
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1060..2085
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2125..2299
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2377..2401
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2826..3620
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3707..4295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4481..4621
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      4965..5159
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5225..6292)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6726..7355)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8655..8679
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8882..8898
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8920..9044
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9069..9099
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9455..9760
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(9801..9925)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9936..10836
                     /label=ZmUbi
     5'UTR           10837..10919
                     /label=ZmUbi1 5'UTR
     intron          10920..11704
                     /label=ZmUbi1 Intron 1
     CDS             11775..11840
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11847..11867
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11892..15992
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9H840A
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    15993..16094
                     /label=Flexible linker
     CDS             16095..18167
                     /codon_start=1
                     /label=MLV-reverse transcriptase
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA
                     DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK
                     KLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKALFLPKRLSIIHC
                     PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPSGGSKRTADGSEFE"
     CDS             18168..18188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      18214..18466
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgataac atggtggagc acgacactct cgtctactcc
      601 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
      661 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
      721 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
      781 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
      841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
      901 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagaccttcc tctatataag
      961 gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat
     1021 ctctctcgag ctttcgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     1081 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     1141 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     1201 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     1261 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggagtttag cgagagcctg
     1321 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     1381 ctgcccgctg ttctacaacc ggtcgcggag gctatggatg cgatcgctgc ggccgatctt
     1441 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     1501 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     1561 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     1621 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     1681 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     1741 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     1801 tacttcgagc ggaggcatcc ggagcttgca ggatcgccac gactccgggc gtatatgctc
     1861 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     1921 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     1981 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     2041 agtggaaacc gacgccccag cactcgtccg agggcaaaga aatagagtag atgccgaccg
     2101 gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa
     2161 gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta
     2221 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     2281 cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt
     2341 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     2401 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     2461 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     2521 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     2581 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac
     2641 cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
     2701 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     2761 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     2821 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct
     2881 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg
     2941 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac
     3001 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact
     3061 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct
     3121 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca
     3181 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc
     3241 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact
     3301 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa
     3361 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga
     3421 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt
     3481 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg
     3541 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt
     3601 tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa cgtgagtttt
     3661 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3841 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4321 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4381 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4441 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     4501 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     4561 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     4621 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4681 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4741 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     4801 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     4861 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     4921 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     4981 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     5041 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     5101 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     5161 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     5221 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     5281 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     5341 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     5401 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     5461 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     5521 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     5581 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     5641 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     5701 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     5761 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     5821 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     5881 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     5941 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     6001 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     6061 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     6121 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
     6181 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
     6241 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgtcacgcc
     6301 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
     6361 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     6421 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     6481 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     6541 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     6601 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     6661 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     6721 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     6781 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     6841 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     6901 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     6961 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     7021 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     7081 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     7141 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     7201 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     7261 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     7321 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     7381 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     7441 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     7501 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     7561 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     7621 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     7681 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     7741 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     7801 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     7861 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     7921 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     7981 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     8041 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     8101 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     8161 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     8221 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     8281 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     8341 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     8401 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     8461 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     8521 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     8581 aaatggacga acggataaac cttttcacgc ccttttaaat atccgttatt ctaataaacg
     8641 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga
     8701 aggcgggaaa cgacaatctg atccaagctc aagctgctct agcattcgcc attcaggctg
     8761 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8821 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8881 tgtaaaacga cggccagtgc caagctagag acggggatca caagtttgta caaaaaagct
     8941 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     9001 cagactacat aatactgtaa aacacaacat atccagtcat attggcggcc gcattaggca
     9061 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     9121 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     9181 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     9241 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     9301 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     9361 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     9421 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     9481 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     9541 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     9601 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     9661 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     9721 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     9781 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag cattatgtag
     9841 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     9901 gttcagcttt cttgtacaaa gtggtgaagc ttgcatgcct gcaggcagcg tgacccggtc
     9961 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat
    10021 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt
    10081 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata
    10141 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac
    10201 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata
    10261 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata
    10321 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa
    10381 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga
    10441 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt
    10501 ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag
    10561 cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc
    10621 ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca
    10681 gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct
    10741 cacggcacgg cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc
    10801 ccgccgtaat aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga
    10861 gcgcacacac acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg
    10921 tacgccgctc gtcctccccc cccccccctc tctaccttct ctagatcggc gttccggtcc
    10981 atggttaggg cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt
    11041 agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt
    11101 gctaacttgc cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac
    11161 gggatcgatt tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt
    11221 tatttcaata tatgccgtgc acttgtttgt cgggtcatct tttcatgctt tttttttgtc
    11281 ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa
    11341 tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca
    11401 tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt
    11461 gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct
    11521 aaccttgagt acctatctat tataataaac aagtatgttt tataattatt ttgatcttga
    11581 tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat
    11641 acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt
    11701 acttctgcag gtcgactcta gaggatctgg aattcccggg taccggatcc gatccaaatc
    11761 cgaatccggc catggactat aaggatcacg atggcgacta caaggatcat gacattgact
    11821 ataaggatga cgacgataag atggcaccta agaagaaaag gaaagtcggc attcatggcg
    11881 ttccggcagc cgacaaaaag tatagcatcg gcctcgatat tgggacaaac tctgtgggct
    11941 gggcggtaat taccgacgag tacaaggtgc ctagtaagaa atttaaagtg ctcggaaaca
    12001 ctgacaggca ctctataaag aagaacctga tcggggcact gcttttcgac tccggagaga
    12061 cggcggaggc gacgcgtctc aagcgtaccg cgcgccgcag gtacacaaga aggaagaata
    12121 ggatctgcta cttgcaggaa atcttcagta acgagatggc gaaggtcgac gatagtttct
    12181 ttcatcggtt ggaagaatcg ttcctcgtag aggaggacaa aaagcacgag cgtcacccaa
    12241 tattcgggaa tattgttgac gaggttgcct accatgagaa atatcctaca atatatcacc
    12301 tccgtaagaa gcttgtcgat tcaactgata aggctgatct cagactcatc tatcttgccc
    12361 tcgcacatat gattaagttt cgtggccact tcttgattga aggcgacctc aacccggaca
    12421 actcagatgt tgacaagctt tttatacagc tcgtccagac atataaccag ctgtttgaag
    12481 agaatcccat caatgcgagt ggggttgatg ctaaggccat tttgtccgcc aggttgtcca
    12541 aatctcgcag actggaaaac ctgatcgcac agcttcccgg tgaaaagaaa aacgggctct
    12601 tcggcaatct catcgcactg tccctcggcc tcaccccaaa cttcaagtct aacttcgacc
    12661 tggccgagga tgcgaagctc cagctgtcaa aagatacata cgacgacgat ttggacaatc
    12721 tgcttgcgca aataggcgac cagtatgcgg acctgttcct ggctgccaaa aatctgtcag
    12781 atgcaatcct cctgtccgat atattgcgtg tgaacaccga aatcacgaag gcaccgctta
    12841 gcgcatccat gatcaagaga tacgacgagc accatcagga cctcacactc ctcaaggcgc
    12901 ttgttcgtca gcagcttccc gagaaatata aggaaatttt tttcgatcaa agcaagaatg
    12961 gatatgctgg ctatattgac ggtggcgctt cgcaggagga gttctataaa ttcattaagc
    13021 cgattctgga gaagatggac ggaacggagg agctcctcgt caagcttaac cgggaagacc
    13081 tgttgcggaa gcagaggact tttgataacg gctctattcc gcaccaaatc catctgggtg
    13141 agttgcacgc aatcttgaga agacaagagg atttctaccc gttccttaag gataacagag
    13201 agaagataga aaaaatactg accttcagga taccatacta tgtgggccca ctggcgcgcg
    13261 gaaatagtcg tttcgcatgg atgactagaa agtccgaaga aacgatcacg ccatggaatt
    13321 ttgaggaagt ggtcgacaag ggcgcctctg cccagagctt catcgaaagg atgaccaatt
    13381 ttgacaaaaa tctgcctaac gaaaaggtgc ttccgaagca cagcctgttg tatgaatact
    13441 tcacagttta taacgagctc actaaggtca agtacgtcac ggagggcatg cgtaagcctg
    13501 ctttcctgtc tggtgaacaa aaaaaggcga ttgtggacct ccttttcaag acgaaccgta
    13561 aagttactgt gaagcaactg aaagaggatt actttaagaa aattgagtgc ttcgacagtg
    13621 tggagatttc cggtgtcgag gaccggttta acgccagcct gggtacgtat catgacctgc
    13681 ttaaaattat caaggataaa gatttcctgg ataatgaaga gaacgaagat atactggagg
    13741 acattgtgtt gactttgacc ctcttcgagg acagagagat gattgaggaa agactgaaga
    13801 cctacgcaca cctttttgat gacaaggtca tgaaacaact caagcgccgg cgctatactg
    13861 gctggggccg gctttctcgc aagctcatca atgggattcg ggataagcaa tcaggcaaga
    13921 caattttgga cttcctcaaa tccgacggat tcgcaaatag gaattttatg cagctgatac
    13981 atgacgactc tttgacattc aaagaagaca tacagaaggc tcaggtctcc ggccaaggag
    14041 attctttgca cgagcatatc gctaacttgg caggtagccc cgccataaaa aagggcattc
    14101 ttcaaacggt aaaagttgtt gacgaactcg tgaaggttat gggccgtcat aagccggaaa
    14161 acattgttat tgaaatggct agggaaaatc agacgaccca gaagggacag aaaaatagca
    14221 gggagcggat gaagagaatt gaagagggaa ttaaggagct tggatctcag attcttaagg
    14281 agcaccctgt ggagaacacc caacttcaga atgaaaagct ctacctttac taccttcaaa
    14341 acggccggga tatgtacgtc gatcaggaac ttgacattaa ccggttgagc gattatgacg
    14401 ttgacgctat tgtgccccaa tctttcctta aagacgactc tatcgacaat aaagtgctga
    14461 cgcgcagcga taaaaatcgc ggtaagtcgg ataatgtccc gtcggaagag gtggttaaaa
    14521 aaatgaagaa ctattggagg caactcctga atgccaagct gatcactcag aggaaattcg
    14581 acaatctcac caaggcagaa aggggtggac ttagcgagct cgacaaggcc ggttttatca
    14641 aaagacagct ggtggagaca cgccaaatca ccaaacacgt tgcccagatc ctggattcga
    14701 ggatgaacac gaagtatgac gagaacgaca agttgattag ggaagtcaag gtcatcactt
    14761 tgaagtccaa gctggtgagc gactttcgca aagacttcca gttttacaaa gtcagggaaa
    14821 ttaataacta ccaccacgcc cacgacgcct accttaacgc cgtggttggc acagcactca
    14881 tcaagaaata ccctaagctc gaatctgagt tcgtctatgg cgactataag gtctacgacg
    14941 ttagaaaaat gatcgcgaaa tctgagcagg aaataggcaa ggcaactgcc aagtacttct
    15001 tctattccaa tatcatgaac ttttttaaga cggagattac cctggcgaat ggtgagatcc
    15061 gcaagcgccc tttgattgag acaaacggag aaacaggaga gatcgtatgg gacaaagggc
    15121 gggactttgc tactgttagg aaggtgctct ctatgccaca agttaacatt gtcaaaaaaa
    15181 ctgaagtgca gacaggtggg tttagcaagg aatctatcct gccgaagagg aactctgaca
    15241 agctgatcgc ccgcaagaaa gattgggatc cgaaaaagta cggaggattc gactccccca
    15301 cagttgcgta ctccgtgctt gtcgtggcca aagtggagaa gggcaagtct aagaagctca
    15361 agagcgtcaa agagttgttg gggatcacga ttatggagcg gtcgtctttc gaaaagaatc
    15421 cgatagattt tctcgaggcc aagggttata aagaagtcaa gaaggatctt atcatcaagc
    15481 tccctaagta ctccctcttt gagcttgaaa acggacggaa aagaatgctg gcttcagcgg
    15541 gtgaacttca gaagggtaat gaactcgctc tgccctcaaa atatgtgaat ttcctttacc
    15601 tggcatcaca ctatgagaag cttaaggggt ctccagagga caacgagcag aagcaactgt
    15661 tcgttgaaca acacaagcac taccttgacg agattatcga gcaaatcagc gagtttagca
    15721 agcgcgttat actggcagac gcaaatcttg ataaggtcct tagcgcctac aacaagcata
    15781 gagacaaacc catccgggag caggccgaga acattattca tctcttcacc ttgacgaatc
    15841 ttggggcccc ggccgcgttc aagtacttcg atactaccat agacagaaag cgctatacat
    15901 cgacaaagga agttcttgac gccacgctga tccaccaaag tataacaggc ctctatgaga
    15961 cacgcatcga cctttcgcag ttgggcggtg acctctctgg cggcagtagt ggcggatcct
    16021 caggaagtga gactcccgga acatccgaat ctgctactcc agagagctca ggtggcagct
    16081 ccggaggtag ctccacactt aacattgaag acgaatatcg cctccatgag acaagtaagg
    16141 aacccgacgt ttcgcttggc tctacctggc tctccgactt cccccaagct tgggccgaaa
    16201 ccggcggtat ggggcttgct gttcgccagg ccccattgat aataccgctg aaagcgacaa
    16261 gcacaccagt ttcgatcaaa caatacccaa tgtctcagga ggcgaggctc ggcattaagc
    16321 cgcacatcca acggttgctc gaccagggta ttcttgtgcc ctgccaatcg ccgtggaaca
    16381 cccccttgtt gcccgttaag aaaccaggaa ccaacgacta caggccggtg caagacttgc
    16441 gcgaggtgaa caaacgggtg gaggatattc acccgactgt ccccaaccca tataatttgc
    16501 tttcaggttt gccccctagc catcagtggt ataccgtgct ggacttgaaa gatgccttct
    16561 tttgtttgcg cctccacccg acttcacagc ccctgtttgc gttcgaatgg cgcgatccgg
    16621 agatgggtat ttcggggcaa cttacgtgga caagactgcc acagggattt aaaaattcgc
    16681 caacactctt caacgaggcc cttcacaggg atcttgccga cttccgcatc cagcaccctg
    16741 accttatcct tctccagtac gtcgacgatt tgcttctcgc agctactagc gaactcgact
    16801 gccagcaagg cacacgggct ctgctccaga cactggggaa ccttggctac cgggcctctg
    16861 ccaaaaaagc gcaaatatgc cagaagcagg ttaagtactt ggggtatttg ctgaaggagg
    16921 ggcaaaggtg gctcacagag gcacgcaaag aaacggtcat gggccagcct accccaaaaa
    16981 caccaagaca actgcgcgag tttttgggta aagcaggatt ttgccggctg tttatcccgg
    17041 gtttcgccga aatggccgca cctttgtatc cgttgactaa gcccggtact ctgtttaact
    17101 ggggacccga ccaacaaaaa gcatatcaag agatcaagca ggcattgctg accgcgccgg
    17161 ccctgggttt gcccgacttg accaaaccgt ttgaattgtt tgttgacgaa aagcagggat
    17221 acgcaaaggg cgttctcacg caaaaactcg gaccttggag aagacccgtt gcatacctta
    17281 gtaaaaagct ggatccggtg gccgctggat ggccaccgtg tttgaggatg gtcgccgcta
    17341 ttgcggtcct tacgaaggat gccggcaaac tgacgatggg gcaaccactt gttattcttg
    17401 cacctcacgc cgtcgaggct cttgttaaac aaccacccga tcgctggctg tctaatgctc
    17461 ggatgaccca ttaccaagct cttttgctgg atacagaccg ggttcaattc gggccggtcg
    17521 tcgccctcaa cccggccact ttgctgccgc tcccagagga aggcctgcaa cacaattgtc
    17581 ttgacattct tgccgaggcc cacggtacaa ggcccgacct caccgaccag cctcttccgg
    17641 acgctgacca tacctggtac acagacggga gcagtcttct gcaagaggga cagaggaaag
    17701 caggggccgc agttaccact gaaactgaag tgatatgggc taaagcattg cccgcgggaa
    17761 ctagcgcaca gcgcgcggag ctcattgctt tgactcaggc actgaaaatg gccgaaggca
    17821 agaagctgaa tgtctacacc gactcgagat atgctttcgc tactgcccat atacatgggg
    17881 agatatatag gcgcagggga tggctcacct ctgaaggtaa ggagattaag aacaaagatg
    17941 agatcctggc tttgctcaaa gctttgttcc tccctaagag gttgtcaata atccattgtc
    18001 ccgggcatca aaaaggtcat tcggcggagg ctaggggtaa caggatggcg gaccaggcag
    18061 ctcgcaaggc cgccattaca gagacacccg atacctcaac tttgttgatc gaaaactcca
    18121 gccccagcgg aggatcaaaa agaaccgcag acggttcgga attcgaaccc aaaaagaaga
    18181 ggaaagtttg aactagtgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
    18241 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
    18301 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
    18361 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
    18421 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggaa attaattc
//
