LOCUS       Exported               18330 bp ds-DNA     circular UNA 22-DEC-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...1366981959514.15
VERSION     .
KEYWORDS    pPPED
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 18330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 2, 2020 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\nz59\Downloads
            Filename :: p201N_Cas9.gb
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..18330
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    644..649
                     /label=StyI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^CWWGG"
                     /note="/annotation_group=CCWWGG"
     misc_feature    978..1978
                     /label=pVS1 Sta
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    2025..2030
                     /label=StyI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^CWWGG"
                     /note="/annotation_group=CCWWGG"
     misc_feature    2485..2490
                     /label=StyI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^CWWGG"
                     /note="/annotation_group=CCWWGG"
     misc_feature    2571..3571
                     /label=pVS1 Rep
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3981..4241
                     /label=pBR322 bom
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    4032..4037
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=CpG:blocked, EcoKI:variable"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     misc_feature    4381..4661
                     /label=pBR322 ori
                     /label=nonstandard type: motif
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    4530..4535
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=CpG:blocked, EcoKI:variable"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     exon            complement(4941..5830)
                     /label=aph
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    5467..5472
                     /label=XmaI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=CpG:some impaired"
                     /note="/Recognition_pattern=C^CCGGG"
                     /note="/annotation_group=CCCGGG"
     misc_feature    5467..5472
                     /label=SmaI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=CpG:blocked"
                     /note="/Recognition_pattern=CCC^GGG"
                     /note="/annotation_group=CCCGGG"
     misc_feature    5684..5689
                     /label=NruI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dam:blocked, CpG:blocked"
                     /note="/Recognition_pattern=TCG^CGA"
                     /note="/annotation_group=TCGCGA"
     misc_feature    6068..6093
                     /label=Left Border
                     /note="/created_by=User"
                     /note="/modified_by=User"
     terminator      complement(6423..6828)
                     /label=StUbi-3 terminator
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    6675..6680
                     /label=EcoRV
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GAT^ATC"
                     /note="/annotation_group=GATATC"
     misc_feature    complement(6852..7646)
                     /label=nptII
                     /note="Geneious type: selectable marker"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    7100..7105
                     /label=StyI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^CWWGG"
                     /note="/annotation_group=CCWWGG"
     promoter        complement(7680..8606)
                     /label=StUbi-3
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        8734..9519
                     /label=2X35S
                     /note="/%_Identity=100"
                     /note="/Mismatches=0"
                     /note="/Motif=CCCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATC
                     AAAGA 
                     TACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGA 
                     AACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAA 
                     AGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGA 
                     TGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAA 
                     AAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAACATGGTGGAG 
                     CACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGG 
                     CTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCC 
                     AGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGC 
                     CATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCA 
                     AAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC 
                     TTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCC 
                     CACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA 
                     CACGCTGAAATCACCAGTCTCTCTCTACAAATCTATCTCT"
                     /note="/annotation_group=35S: 8,697 -> 9,482"
                     /note="/modified_by=User"
     misc_feature    9386..9391
                     /label=EcoRV
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GAT^ATC"
                     /note="/annotation_group=GATATC"
     misc_feature    9539..9559
                     /label=SV40 NLS
                     /label=nonstandard type: SV40
                     /note="/created_by=nz59"
                     /note="/modified_by=nz59"
     gene            9566..13666
                     /label=AtCas9H840A
                     /note="/created_by=nz59"
     misc_feature    9813..9818
                     /label=BglII
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dam:cut, EcoBI:some blocked"
                     /note="/Recognition_pattern=A^GATCT"
                     /note="/annotation_group=AGATCT"
     misc_feature    11236..11242
                     /label=BstEII
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dcm:cut, CpG:cut, EcoBI:impaired, 
                     EcoKI:impaired"
                     /note="/Recognition_pattern=G^GTNACC"
                     /note="/annotation_group=GGTNACC"
     misc_feature    13377..13382
                     /label=BglII
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dam:cut, EcoBI:some blocked"
                     /note="/Recognition_pattern=A^GATCT"
                     /note="/annotation_group=AGATCT"
     misc_feature    13667..13768
                     /label=Flexible linker
                     /label=nonstandard type: polylinker
                     /note="/created_by=nz59"
     unsure          13769..15841
                     /label=MLV reverse transcriptase
                     /note="/created_by=nz59"
     misc_feature    14029..14034
                     /label=KpnI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dcm:cut, CpG:cut"
                     /note="/Recognition_pattern=GGTAC^C"
                     /note="/annotation_group=GGTACC"
     misc_feature    14328..14334
                     /label=BstEII
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dcm:cut, CpG:cut, EcoBI:impaired, 
                     EcoKI:impaired"
                     /note="/Recognition_pattern=G^GTNACC"
                     /note="/annotation_group=GGTNACC"
     misc_feature    15854..15874
                     /label=SV40 NLS
                     /label=nonstandard type: SV40
                     /note="/created_by=nz59"
                     /note="/modified_by=nz59"
     terminator      15933..16203
                     /label=nos terminator
                     /note="/%_Identity=100"
                     /note="/Mismatches=0"
                     /note="/Motif=ATTTCCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGAT
                     TGAAT 
                     CCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATG 
                     TAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGT 
                     CCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGAT 
                     AAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTGAT"
                     /note="/annotation_group=nos terminator: 13,691 -> 13,961"
                     /note="/modified_by=User"
     protein_bind    16449..16573
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        16610..16640
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             16694..17353
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             17695..18000
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(18041..18165)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    complement(18192..18216)
                     /label=Right Border
                     /note="/created_by=User"
                     /note="/modified_by=User"
ORIGIN
        1 agtactttaa agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat
       61 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
      121 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
      181 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
      241 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
      301 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
      361 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
      421 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
      481 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
      541 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
      601 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
      661 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
      721 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
      781 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
      841 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
      901 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
      961 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     1021 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     1081 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     1141 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     1201 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     1261 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     1321 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     1381 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     1441 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     1501 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     1561 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     1621 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     1681 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     1741 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     1801 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     1861 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     1921 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     1981 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     2041 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     2101 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     2161 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     2221 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     2281 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     2341 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     2401 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     2461 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     2521 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     2581 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     2641 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     2701 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     2761 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     2821 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     2881 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     2941 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     3001 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     3061 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     3121 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     3181 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     3241 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     3301 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     3361 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     3421 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     3481 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     3541 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     3601 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     3661 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     3721 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     3781 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     3841 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3901 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     3961 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     4021 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4081 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4681 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4921 gttaagggat tttggtcatg caggatcatg aattaattct tagaaaaact catcgagcat
     4981 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     5041 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     5101 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     5161 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     5221 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     5281 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     5341 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     5401 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     5461 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     5521 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     5581 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     5641 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     5701 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
     5761 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     5821 tcatgatctg gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
     5881 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
     5941 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
     6001 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     6061 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     6121 cattgcggac gtttttaatg tactgaatta acgccgaatt gctctagcca atacgcaaac
     6181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6241 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6301 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6361 ttcacacagg aaacagctat gacatgatta cgaattcctt gactagaggg taggcgcggc
     6421 ttaattaagc cgcgccgtac cccaatagtc tcgacagaca catagcacct aacattattg
     6481 cttaaattta ctattttttt actatattat actcaaccca atgagcataa agactgtaaa
     6541 atctcaaatt cctgagaagc atatttatcg atcccacaga cttgatagtt ccataatcca
     6601 tacgctgcag ccaaattgct agtgtgttga acatttaaca cgtagagaac tagaaaagat
     6661 ataaaactaa gattgatatc caaaatagac gagaacaata agcaaaaact cttagttttg
     6721 aaataaatca acaatcccga gggttgtcac atacatcaaa aacgaaaatc catatagcaa
     6781 aaaaaactct aaattaccgt tcgacaaaaa gagaaaactg ataggacatt tgctaaacat
     6841 taaaatcaaa atttggatca agccgaattc tcagaagaac tcgtcaagaa ggcgatagaa
     6901 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     6961 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     7021 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     7081 attcggcaag caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc
     7141 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     7201 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     7261 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     7321 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     7381 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     7441 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
     7501 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     7561 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     7621 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatctcga gacgtaccgc
     7681 gcgatgcatt cgaagatcga tccttcgcct ggaggagaga aatcagtggc gctgcggctt
     7741 ttagggtttc tttgttgatg gaatgagagt gtaagctctg ccagtgccac tttattaggg
     7801 ttttacaagc ccttttcttc gtaattgggc ctgacatttt gtgccacttg ggcctttaga
     7861 gatgaaaatg tatattgggc ttaagttgac ttgaaggata aattagttta ggatattacg
     7921 ttttttatga gaattggtgt gtcggataca tgtatatgat gcattcaaat atatgtattc
     7981 tagatacatt taagtttaga tacaatctaa aatgtgtctt taattacagg actgtaacta
     8041 aaatacttaa tgtaagaaga atattactcc tttaatagct tttgagtata tctagtctaa
     8101 catcttttaa aaaagtctaa tttctttcat ttatttttcg agcaatagca aagtgcataa
     8161 ttattttttt cttctagaaa ttcagatttg tttctctaaa ttttgagatt cttttctcaa
     8221 ttttgtatgt ctagagaaca atgtgtattt ttcactctag ttggttgtta ctttgttgaa
     8281 tgttctgata aaagtatatt gttatttctg aagtagatat aaaccttcat ttggaaatta
     8341 tacataaatc aaaatcgtta attatctaga tcaagatata tgcccttttc ctaatgtatt
     8401 tgatacatgc acctaatttc actagatgta tcttttctat tttttaaatt atgaatagtt
     8461 aattttttcc atatgtgtat ttgatacata cttcatgact ttaaaaaatt aattatatac
     8521 cagatatatg tatttaaaat ttgttatgta tttaaagtat gtatatgatt attcgatatt
     8581 aatctcttcg atgaaattta aatcgataac tatgtgcttt ggatcgatct gcccactagt
     8641 gagtcgtatt acaattcatg actctcttaa ggtagccaaa atcgatatct agggataaca
     8701 gggtaatcga tcactagaat tcggcgcgcc gggcccaaca tggtggagca cgacactctc
     8761 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
     8821 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     8881 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
     8941 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     9001 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     9061 gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
     9121 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
     9181 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
     9241 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
     9301 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
     9361 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     9421 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     9481 acgctgaaat caccagtctc tctctacaaa tctatctctc tcgaccgcta gcatggcacc
     9541 taagaaaaag aggaaggttg gtattgataa gaagtactct atcggactcg atatcggaac
     9601 taactctgtg ggatgggctg tgatcaccga tgagtacaag gtgccatcta agaagttcaa
     9661 ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg ctctcctctt
     9721 cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa gaaggtacac
     9781 cagaagaaag aacaggatct gctacctcca agagatcttc tctaacgaga tggctaaagt
     9841 ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag ataagaagca
     9901 cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg agaagtaccc
     9961 tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg atctcaggct
    10021 catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca tcgagggtga
    10081 tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc agacctacaa
    10141 ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg ctatcctctc
    10201 tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc ctggtgagaa
    10261 gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc ctaacttcaa
    10321 gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata cctacgatga
    10381 tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt tcctcgctgc
    10441 taagaacctc tctgatgcta tcctcctcag tgatatcctc agagtgaaca ccgagatcac
    10501 caaggctcca ctctcagctt ctatgatcaa gagatacgat gagcaccacc aggatctcac
    10561 acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaga ttttcttcga
    10621 tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag aagagttcta
    10681 caagttcatc aagcctatcc tcgagaagat ggatggaacc gaggaactcc tcgtgaagct
    10741 caatagagag gatcttctca gaaagcagag gaccttcgat aacggatcta tccctcatca
    10801 gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct acccattcct
    10861 caaggataac agggaaaaga ttgagaagat tctcaccttc agaatccctt actacgtggg
    10921 acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg aggaaaccat
    10981 caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt ctttcatcga
    11041 gaggatgacc aacttcgata agaaccttcc aaacgagaag gtgctcccta agcactcttt
    11101 gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg tgaccgaggg
    11161 aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg atctcttgtt
    11221 caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca agaaaatcga
    11281 gtgcttcgat tcagttgaga tttctggtgt tgaggatagg ttcaacgcat ctctcggaac
    11341 ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg aggaaaacga
    11401 ggatatcttg gaggatatcg ttcttaccct caccctcttt gaagatagag agatgattga
    11461 agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc agttgaagag
    11521 aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa tcagggataa
    11581 gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta acagaaactt
    11641 catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga aggctcaggt
    11701 ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat ctcctgcaat
    11761 caagaaggga atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg tgatgggaag
    11821 gcataagcct gagaacatcg tgatcgaaat ggctagagag aaccagacca ctcagaaggg
    11881 acagaagaac tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag agcttggatc
    11941 tcagatcctc aaagagcacc ctgttgagaa cactcagctc cagaatgaga agctctacct
    12001 ctactacctc cagaacggaa gggatatgta tgtggatcaa gagttggata tcaacaggct
    12061 ctctgattac gatgttgatg ctatcgtgcc acagtcattc ttgaaggatg attctatcga
    12121 taacaaggtg ctcaccaggt ctgataagaa caggggtaag agtgataacg tgccaagtga
    12181 agaggttgtg aagaaaatga agaactattg gaggcagctc ctcaacgcta agctcatcac
    12241 tcagagaaag ttcgataact tgactaaggc tgagagggga ggactctctg aattggataa
    12301 ggcaggattc atcaagaggc agcttgtgga aaccaggcag atcactaagc acgttgcaca
    12361 gatcctcgat tctaggatga acaccaagta cgatgagaac gataagttga tcagggaagt
    12421 gaaggttatc accctcaagt caaagctcgt gtctgatttc agaaaggatt tccaattcta
    12481 caaggtgagg gaaatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt
    12541 tggaaccgct ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt acggtgatta
    12601 caaggtgtac gatgtgagga agatgatcgc taagtctgag caagagatcg gaaaggctac
    12661 cgctaagtat ttcttctact ctaacatcat gaatttcttc aagaccgaga ttaccctcgc
    12721 taacggtgag atcagaaaga ggccactcat cgagacaaac ggtgaaacag gtgagatcgt
    12781 gtgggataag ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc cacaggtgaa
    12841 catcgttaag aaaaccgagg tgcagaccgg tggattctct aaagagtcta tcctccctaa
    12901 gaggaactct gataagctca ttgctaggaa gaaggattgg gaccctaaga aatacggtgg
    12961 tttcgattct cctaccgtgg cttactctgt tctcgttgtg gctaaggttg agaagggaaa
    13021 gagtaagaag ctcaagtctg ttaaggaact tctcggaatc actatcatgg aaaggtcatc
    13081 tttcgagaag aacccaatcg atttcctcga ggctaaggga tacaaagagg ttaagaagga
    13141 tctcatcatc aagctcccaa agtactcact cttcgaactc gagaacggta gaaagaggat
    13201 gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt gctctcccat ctaagtacgt
    13261 taactttctt tacctcgctt ctcactacga gaagttgaag ggatctccag aagataacga
    13321 gcagaagcaa cttttcgttg agcagcacaa gcactacttg gatgagatca tcgagcagat
    13381 ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg tgttgtctgc
    13441 ttacaacaag cacagagata agcctatcag ggaacaggca gagaacatca tccatctctt
    13501 cacccttacc aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa ccatcgatag
    13561 gaagagatac acctctacca aagaagtgct cgatgctacc ctcatccatc agtctatcac
    13621 tggactctac gagactagga tcgatctctc acagctcggt ggtgatctca gtggtgggag
    13681 cagtggtgga tcatcaggga gtgagactcc aggtacaagt gaaagcgcta ctcctgaatc
    13741 ttcaggaggt tcctcaggag gaagttctac tcttaacatc gaggacgagt atcgtctcca
    13801 cgagaccagc aaggaacctg atgtatctct aggatcaact tggctcagtg atttccccca
    13861 ggcatgggca gaaacaggtg gcatgggttt ggctgtacgt caagcaccac ttataattcc
    13921 tctaaaagca acaagcaccc ccgtcagtat taagcagtac ccaatgagtc aggaggctag
    13981 attgggcatt aagccacaca tacagagact tttagatcaa ggcattctgg taccctgtca
    14041 gtccccctgg aacacaccac tgctgcctgt taagaaacca ggaaccaacg attaccgacc
    14101 agttcaggac ttgcgagaag tgaacaaaag ggtcgaggat atccacccta ccgtgcctaa
    14161 tccatacaac cttttatctg gccttcctcc aagccatcaa tggtacaccg ttctagattt
    14221 gaaagatgca ttcttttgtc tgcgactcca ccccacctca cagccccttt tcgccttcga
    14281 gtggagagac cctgaaatgg gtatcagcgg ccaacttact tggactaggt taccccaggg
    14341 atttaaaaac agtccaaccc tattcaatga ggcattgcac agggacctgg cagattttcg
    14401 tatacaacac cccgatctaa tattgttaca atacgtggac gaccttctcc tagctgcaac
    14461 aagcgagtta gattgtcagc aaggaacaag ggctcttttg caaacactag gaaacttggg
    14521 gtatcgtgct agtgccaaaa aagctcagat ttgtcaaaag caggtaaagt acttagggta
    14581 tttactaaag gaaggtcaga gatggttaac cgaagcccga aaggaaactg taatggggca
    14641 accaactcct aaaactccca gacaactcag ggagttcttg ggcaaagcag ggttttgtag
    14701 acttttcata cccggctttg ctgaaatggc agcacccctg tatcccttga caaagcctgg
    14761 gacactattt aactgggggc cagaccagca gaaggcatac caagaaataa agcaagcact
    14821 actcaccgca cctgccctag gcctgcctga tctgactaaa ccatttgaac tcttcgtaga
    14881 tgagaaacaa ggctacgcta aaggtgtgct gacacaaaaa ttgggaccct ggcgacgacc
    14941 agtcgcctac ctcagcaaga aattagaccc cgtggccgcc ggttggccac catgcttacg
    15001 aatggtggca gcaatcgccg tcctgacaaa ggatgcaggc aaacttacca tgggccaacc
    15061 tctagtcatc ttagcacccc atgccgttga ggctctcgta aagcagccac ctgatagatg
    15121 gctcagtaac gctcgaatga cccactatca agccctcctc ttggataccg acagagtcca
    15181 gttcggaccc gtagtagcac ttaacccagc aacactgcta cccctccctg aggaagggct
    15241 acaacataac tgcctagaca ttttggccga ggcacatggg acacgtccag acttgacaga
    15301 tcagccctta ccagacgccg accacacatg gtacacagac ggaagttcac tcttgcagga
    15361 gggtcagagg aaggccgggg ctgctgtgac taccgagacc gaagttattt gggctaaggc
    15421 cctaccagcc ggcacctctg cacagagagc agagttaatt gcactaaccc aggccctcaa
    15481 aatggccgaa ggtaaaaaac taaatgtcta tacagattca cgatatgctt ttgcaacagc
    15541 ccatatacat ggcgaaattt ataggaggag ggggtggtta acatcagagg gaaaagagat
    15601 aaaaaacaag gacgaaatcc ttgcattgct aaaggcttta ttccttccca agcgtttaag
    15661 cataatccac tgtcccggtc atcagaaggg tcactccgcc gaggcaagag gcaaccgaat
    15721 ggctgaccaa gccgcaagga aggccgcaat cactgagacc ccagacacct ctactctact
    15781 aatcgaaaac agctcccctt caggtggaag caagagaacc gccgacggca gtgaattcga
    15841 aagcagggct gaccccaaga agaagaggaa ggtgtgaccg cggatatctg cagaagctta
    15901 cgcgtcgacg tctcgaggcc ggccgagctc gaatttcccc gatcgttcaa acatttggca
    15961 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
    16021 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
    16081 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
    16141 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
    16201 gatatctagg gataacaggg taataagctt ggcactggcc gtcgttttac aacgtcgtga
    16261 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
    16321 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
    16381 tggcgaatga gcttgagctt ggatcagatt gtcgtttccc gccttcagtt tggactctag
    16441 agttatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
    16501 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
    16561 tccagtcact atggcggccc ctcgaggggc cgcattaggc accccaggct ttacacttta
    16621 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
    16681 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
    16741 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
    16801 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
    16861 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
    16921 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
    16981 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
    17041 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
    17101 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
    17161 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
    17221 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
    17281 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
    17341 gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt atgcgtattt
    17401 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
    17461 aagaggtgtg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
    17521 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
    17581 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
    17641 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag
    17701 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
    17761 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
    17821 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
    17881 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
    17941 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
    18001 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
    18061 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
    18121 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgata acagcggaaa
    18181 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
    18241 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
    18301 tgccaaccac agggttcccc tcgggatcaa
//
