LOCUS       pEGFP-C1                5523 bp    DNA     circular SYN  3-MAR-2016
DEFINITION  Cloning vector pEGFP-C1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55763
VERSION     U55763.1  GI:1377914
SOURCE      Cloning vector pEGFP-C1.
  ORGANISM  Cloning vector pEGFP-C1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1 SPEC NO), 33-38 (1996)
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-C1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4731)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11133845|
COMMENT     VNTDBDATE|-11133845|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                            5 "ACCGGT" "TGGCCA") 
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COMMENT                            7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
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COMMENT                            5 1 "GGTACC" "CCATGG") 
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COMMENT                            2 4 "CATATG" "GTATAC") 
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COMMENT                            5 1 "CTGCAG" "GACGTC") 
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COMMENT                            5 1 "GAGCTC" "CTCGAG") 
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COMMENT                            1 5 "GTCGAC" "CAGCTG") 
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COMMENT                            3 3 "CCCGGG" "GGGCCC") 
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COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Cloning vector pEGFP-C1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:47946"
                     /vntifkey="98"
     gene            672..2202
                     /gene="egfp"
                     /vntifkey="60"
     CDS             3419..4213
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02577.1"
                     /db_xref="GI:1377916"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    672..1388
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    603..665
                     /vntifkey="21"
                     /label=Signal\peptide\of\Tac
     misc_feature    2052..2108
                     /vntifkey="21"
                     /label=TMD\of\Tac
     misc_feature    2109..2147
                     /vntifkey="21"
                     /label=Cytosolic\tail\of\Tac
     misc_feature    1395..2051
                     /vntifkey="21"
                     /label=Extracellular\domain\of\Tac
     misc_feature    2037..2039
                     /vntifkey="21"
                     /label=T215A
     misc_feature    2040..2042
                     /vntifkey="21"
                     /label=T216A
     misc_feature    2016..2018
                     /vntifkey="21"
                     /label=T208A
     misc_feature    2001..2003
                     /vntifkey="21"
                     /label=T203A
     misc_feature    1983..1985
                     /vntifkey="21"
                     /label=T197A
BASE COUNT     1375 a      1484 c      1456 g      1208 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca 
      601 ccatggattc atacctgctg atgtggggac tgctcacgtt catcatggtg cctggctgcc 
      661 aggcaaccgg tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 
      721 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 
      781 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 
      841 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 
      901 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 
      961 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 
     1021 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 
     1081 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 
     1141 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 
     1201 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 
     1261 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 
     1321 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 
     1381 tgtacaagct cgaggagctc tgtgacgatg acccgccaga gatcccacac gccacattca 
     1441 aagccatggc ctacaaggaa ggaaccatgt tgaactgtga atgcaagaga ggtttccgca 
     1501 gaataaaaag cgggtcactc tatatgctct gtacaggaaa ctctagccac tcgtcctggg 
     1561 acaaccaatg tcaatgcaca agctctgcca ctcggaacac aacgaaacaa gtgacacctc 
     1621 aacctgaaga acagaaagaa aggaaaacca cagaaatgca aagtccaatg cagccagtgg 
     1681 accaagcgag ccttccaggt cactgcaggg aacctccacc atgggaaaat gaagccacag 
     1741 agagaattta tcatttcgtg gtggggcaga tggtttatta tcagtgcgtc cagggataca 
     1801 gggctctaca cagaggtcct gctgagagcg tctgcaaaat gacccacggg aagacaaggt 
     1861 ggacccagcc ccagctcata tgcacaggtg aaatggagac cagtcagttt ccaggtgaag 
     1921 agaagcctca ggcaagcccc gaaggccgtc ctgagagtga gacttcctgc ctcgtcacaa 
     1981 cagcagattt tcaaatacag gcagaaatgg ctgcagccat ggagacgtcc atatttgcag 
     2041 cagagtacca ggtagcagtg gccggctgtg ttttcctgct gatcagcgtc ctcctcctga 
     2101 gtgggctcac ctggcagcgg agacagagga agagtagaag aacaatctag gaattctgca 
     2161 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat 
     2221 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 
     2281 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 
     2341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     2401 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 
     2461 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 
     2521 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 
     2581 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 
     2641 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 
     2701 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 
     2761 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 
     2821 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 
     2881 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     2941 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     3001 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 
     3061 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 
     3121 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 
     3181 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 
     3241 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     3301 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     3361 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 
     3421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     3481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     3541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     3601 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     3661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     3721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     3781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     3841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     3901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 
     3961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     4021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     4081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     4141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     4201 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 
     4261 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 
     4321 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 
     4381 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 
     4441 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 
     4501 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 
     4561 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 
     4621 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 
     4681 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     4741 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     4801 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     4861 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     4921 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 
     4981 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     5041 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     5101 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     5161 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     5221 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     5281 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     5341 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     5401 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     5461 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 
     5521 cat 
//
