LOCUS       pEGFP-C1                4866 bp    DNA     circular SYN  3-MAR-2016
DEFINITION  Cloning vector pEGFP-C1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55763
VERSION     U55763.1  GI:1377914
SOURCE      Cloning vector pEGFP-C1.
  ORGANISM  Cloning vector pEGFP-C1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1 SPEC NO), 33-38 (1996)
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-C1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4731)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11133845|
COMMENT     VNTDBDATE|-11133845|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Cloning vector pEGFP-C1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds." 
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FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Cloning vector pEGFP-C1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:47946"
                     /vntifkey="98"
     gene            672..1545
                     /gene="egfp"
                     /vntifkey="60"
     CDS             2762..3556
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02577.1"
                     /db_xref="GI:1377916"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    672..1388
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    603..665
                     /vntifkey="21"
                     /label=Signal\peptide\of\Tac
     misc_feature    1395..1451
                     /vntifkey="21"
                     /label=TMD\of\Tac
     misc_feature    1452..1490
                     /vntifkey="21"
                     /label=Cytosolic\tail\of\Tac
     misc_feature    1071..1079
                     /vntifkey="21"
                     /label=N-glycosylation\site
BASE COUNT     1168 a      1314 c      1289 g      1095 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca 
      601 ccatggattc atacctgctg atgtggggac tgctcacgtt catcatggtg cctggctgcc 
      661 aggcaaccgg tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 
      721 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 
      781 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 
      841 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 
      901 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 
      961 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 
     1021 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag aacggcagca 
     1081 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 
     1141 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 
     1201 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 
     1261 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 
     1321 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 
     1381 tgtacaagct cgaggtagca gtggccggct gtgttttcct gctgatcagc gtcctcctcc 
     1441 tgagtgggct cacctggcag cggagacaga ggaagagtag aagaacaatc taggaattct 
     1501 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc 
     1561 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 
     1621 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 
     1681 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     1741 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat 
     1801 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 
     1861 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 
     1921 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 
     1981 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 
     2041 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 
     2101 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 
     2161 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 
     2221 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
     2281 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
     2341 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 
     2401 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 
     2461 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 
     2521 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 
     2581 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 
     2641 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 
     2701 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 
     2761 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     2821 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     2881 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     2941 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     3001 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     3061 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     3121 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     3181 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     3241 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 
     3301 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     3361 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     3421 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     3481 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     3541 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     3601 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     3661 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     3721 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 
     3781 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 
     3841 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 
     3901 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 
     3961 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 
     4021 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 
     4081 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 
     4141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 
     4201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 
     4261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 
     4321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 
     4381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 
     4441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 
     4501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 
     4561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 
     4621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 
     4681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 
     4741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 
     4801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 
     4861 atgcat 
//
