LOCUS       pEGFP-C1                5655 bp    DNA     circular SYN 28-NOV-2019
DEFINITION  Cloning vector pEGFP-C1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55763
VERSION     U55763.1  GI:1377914
SOURCE      Cloning vector pEGFP-C1.
  ORGANISM  Cloning vector pEGFP-C1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1 SPEC NO), 33-38 (1996)
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-C1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4731)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11133845|
COMMENT     VNTDBDATE|-13099355|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEGFP-C1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Cloning vector pEGFP-C1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds." 
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COMMENT                          "Created: 03/03/16 06:20PM" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Cloning+vector+pEGFP-C1\">Cloning vector pEGFP-C1(Cloning vector pEGFP-C1)</a>" 
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COMMENT                                   -10.454 0 -10 3.69651 0.799245 #340#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (2150)" "@N (@S)" 5 11.7136 
COMMENT                                   -22.6503 0 -10 3.69651 0.799245 #342#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (3409)" "@N (@S)" 5 -10.3081 
COMMENT                                   -10.454 0 -10 3.97125 0.799245 #344#) 
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COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (4112)" "@N (@S)" 5 -14.2238 
COMMENT                                   -1.74233 0 -10 3.62158 0.799245 #346#)) 
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COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList #350=(XLATFRAGMENT 602 1683 0 1 603 5051)) 
COMMENT        (CObList #351=(AnlzXLATItem 602 5653 3 602 5653)) 1 (CObList)) (CObList) 
COMMENT       0 (CStringList) 14 32888 (CObList)))
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Cloning vector pEGFP-C1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:47946"
                     /vntifkey="98"
     gene            672..2334
                     /gene="egfp"
                     /vntifkey="60"
     CDS             3551..4345
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02577.1"
                     /db_xref="GI:1377916"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    672..1388
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    603..665
                     /vntifkey="21"
                     /label=Signal\peptide\of\Tac
     misc_feature    2184..2240
                     /vntifkey="21"
                     /label=TMD\of\Tac
     misc_feature    2241..2279
                     /vntifkey="21"
                     /label=Cytosolic\tail\of\Tac
     misc_feature    1527..2183
                     /vntifkey="21"
                     /label=Extracellular\domain\of\Tac
     misc_feature    2169..2171
                     /vntifkey="21"
                     /label=T215A
     misc_feature    2172..2174
                     /vntifkey="21"
                     /label=T216A
     misc_feature    2148..2150
                     /vntifkey="21"
                     /label=T208A
     misc_feature    2133..2135
                     /vntifkey="21"
                     /label=T203A
     misc_feature    2115..2117
                     /vntifkey="21"
                     /label=T197A
     misc_feature    2103..2183
                     /vntifkey="21"
                     /label=Tac\stem\region\with\mutated\glycosylation\sites
     misc_feature    1407..1409
                     /vntifkey="21"
                     /label=138\glycosylation\site
     misc_feature    1410..1412
                     /vntifkey="21"
                     /label=139\glycosylation\site
     misc_feature    1413..1415
                     /vntifkey="21"
                     /label=140\glycosylation\site
     misc_feature    1434..1436
                     /vntifkey="21"
                     /label=147\glycosylation\site
     misc_feature    1446..1448
                     /vntifkey="21"
                     /label=151\glycosylation\site
     misc_feature    1455..1457
                     /vntifkey="21"
                     /label=154\glycosylation\site
     misc_feature    1467..1469
                     /vntifkey="21"
                     /label=158\glycosylation\site
     misc_feature    1395..1526
                     /vntifkey="21"
                     /label=134-177\CD8a\stem\region
BASE COUNT     1397 a      1541 c      1501 g      1216 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca 
      601 ccatggattc atacctgctg atgtggggac tgctcacgtt catcatggtg cctggctgcc 
      661 aggcaaccgg tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 
      721 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 
      781 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 
      841 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 
      901 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 
      961 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 
     1021 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 
     1081 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 
     1141 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 
     1201 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 
     1261 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 
     1321 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 
     1381 tgtacaagct cgagccagcg aagcccacca cgacgccagc gccgcgacca ccaacaccgg 
     1441 cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg ccagcggcgg 
     1501 ggggcgcagt gcacacgagg gggctggagc tctgtgacga tgacccgcca gagatcccac 
     1561 acgccacatt caaagccatg gcctacaagg aaggaaccat gttgaactgt gaatgcaaga 
     1621 gaggtttccg cagaataaaa agcgggtcac tctatatgct ctgtacagga aactctagcc 
     1681 actcgtcctg ggacaaccaa tgtcaatgca caagctctgc cactcggaac acaacgaaac 
     1741 aagtgacacc tcaacctgaa gaacagaaag aaaggaaaac cacagaaatg caaagtccaa 
     1801 tgcagccagt ggaccaagcg agccttccag gtcactgcag ggaacctcca ccatgggaaa 
     1861 atgaagccac agagagaatt tatcatttcg tggtggggca gatggtttat tatcagtgcg 
     1921 tccagggata cagggctcta cacagaggtc ctgctgagag cgtctgcaaa atgacccacg 
     1981 ggaagacaag gtggacccag ccccagctca tatgcacagg tgaaatggag accagtcagt 
     2041 ttccaggtga agagaagcct caggcaagcc ccgaaggccg tcctgagagt gagacttcct 
     2101 gcctcgtcac aacagcagat tttcaaatac aggcagaaat ggctgcagcc atggagacgt 
     2161 ccatatttgc agcagagtac caggtagcag tggccggctg tgttttcctg ctgatcagcg 
     2221 tcctcctcct gagtgggctc acctggcagc ggagacagag gaagagtaga agaacaatct 
     2281 aggaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc 
     2341 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 
     2401 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 
     2461 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     2521 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag 
     2581 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca 
     2641 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 
     2701 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 
     2761 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 
     2821 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 
     2881 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 
     2941 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 
     3001 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac 
     3061 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     3121 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg 
     3181 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 
     3241 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 
     3301 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 
     3361 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 
     3421 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 
     3481 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg 
     3541 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 
     3601 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 
     3661 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 
     3721 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 
     3781 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 
     3841 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 
     3901 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 
     3961 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 
     4021 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag 
     4081 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 
     4141 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 
     4201 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 
     4261 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 
     4321 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 
     4381 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 
     4441 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 
     4501 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa 
     4561 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt 
     4621 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc 
     4681 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt 
     4741 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac 
     4801 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 
     4861 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 
     4921 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 
     4981 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 
     5041 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 
     5101 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 
     5161 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 
     5221 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 
     5281 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 
     5341 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 
     5401 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 
     5461 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 
     5521 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 
     5581 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 
     5641 attaccgcca tgcat 
//
