LOCUS       pEGFP-N1                6005 bp    DNA     circular SYN 23-MAR-2007
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55762
VERSION     U55762.1  GI:1377911
SOURCE      Cloning vector pEGFP-N1.
  ORGANISM  Cloning vector pEGFP-N1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1), 33-38 (1996)
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|441652552|
COMMENT     VNTDBDATE|441652552|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEGFP-N1|
COMMENT     VNTAUTHORNAME|LL|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                                   "{\\i Nco}I (361)" "@N (@S)" 5 7.80165 
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FEATURES             Location/Qualifiers
     CDS             3901..4695
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV-F
     misc_feature    1951..2670
                     /vntifkey="21"
                     /label=moxGFP
     misc_feature    625..651
                     /vntifkey="21"
                     /label=ST\Cyto
     misc_feature    652..702
                     /vntifkey="21"
                     /label=ST\TMD
     misc_feature    703..804
                     /vntifkey="21"
                     /label=ST\stem\region
     misc_feature    898..1935
                     /vntifkey="21"
                     /label=ST\Catalytic\domain
     misc_feature    877..879
                     /vntifkey="21"
                     /label=T215A
     misc_feature    880..882
                     /vntifkey="21"
                     /label=T216A
     misc_feature    856..858
                     /vntifkey="21"
                     /label=T208A
     misc_feature    841..843
                     /vntifkey="21"
                     /label=T203A
     misc_feature    823..825
                     /vntifkey="21"
                     /label=T197A
     misc_feature    811..891
                     /vntifkey="21"
                     /label=Tac\stem\region\with\mutated\glycosylation\sites
BASE COUNT     1489 a      1627 c      1540 g      1349 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatgatt cacaccaacc tgaagaaaaa gttcagctgc 
      661 tgcgtcctgg tctttcttct gtttgcagtc atctgtgtgt ggaaggaaaa gaagaaaggg 
      721 agttactatg attcctttaa attgcaaacc aaggaattcc aggtgttaaa gagtctgggg 
      781 aaattggcca tggggtctga ttccccgcgg ctcgtcacaa cagcagattt tcaaatacag 
      841 gcagaaatgg ctgcagccat ggagacgtcc atatttgcag cagagtacca gggatcccag 
      901 tctgtatcct caagcagcac ccaggacccc cacaggggcc gccagaccct cggcagtctc 
      961 agaggcctag ccaaggccaa accagaggcc tccttccagg tgtggaacaa ggacagctct 
     1021 tccaaaaacc ttatccctag gctgcaaaag atctggaaga attacctaag catgaacaag 
     1081 tacaaagtgt cctacaaggg gccaggacca ggcatcaagt tcagtgcaga ggccctgcgc 
     1141 tgccacctcc gggaccatgt gaatgtatcc atggtagagg tcacagattt tcccttcaat 
     1201 acctctgaat gggagggtta tctgcccaag gagagcatta ggaccaaggc tgggccttgg 
     1261 ggcaggtgtg ctgttgtgtc gtcagcggga tctctgaagt cctcccaact aggcagagaa 
     1321 atcgatgatc atgacgcagt cctgaggttt aatggggcac ccacagccaa cttccaacaa 
     1381 gatgtgggca caaaaactac cattcgcctg atgaactctc agttggttac cacagagaag 
     1441 cgcttcctca aagacagttt gtacaatgaa ggaatcctaa ttgtatggga cccatctgta 
     1501 taccactcag atatcccaaa gtggtaccag aatccggatt ataatttctt taacaactac 
     1561 aagacttatc gtaagctgca ccccaatcag cccttttaca tcctcaagcc ccagatgcct 
     1621 tgggagctat gggacattct tcaagaaatc tccccagaag agattcagcc aaacccccca 
     1681 tcctctggga tgcttggtat catcatcatg atgacgctgt gtgaccaggt ggatatttat 
     1741 gagttcctcc catccaagcg caagactgac gtgtgctact actaccagaa gttcttcgat 
     1801 agtgcctgca cgatgggtgc ctaccacccg ctgctctatg agaagaattt ggtgaagcat 
     1861 ctcaaccagg gcacagatga ggacatctac ctgcttggaa aagccacact gcctggcttc 
     1921 cggaccattc actgcgcacc ggtcgccacc atggtgtcca agggcgagga gctgttcacc 
     1981 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttctccgtg 
     2041 cggggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catcagcacc 
     2101 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 
     2161 agcttctccc gctaccccga ccacatgaag cgccacgact tcttcaagag cgccatgccc 
     2221 gaaggctacg tccaggagcg caccatctcc ttcaaggacg acggcaccta caagacccgc 
     2281 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 
     2341 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa ctcccacaac 
     2401 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac 
     2461 aacgtggagg acggctccgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 
     2521 gacggccccg tgctgctgcc cgacaaccac tacctgtcca cccagtccaa gctgtccaaa 
     2581 gaccccaacg agaagcgcga tcacatggtc cttctggaat tcgtgaccgc cgccgggatc 
     2641 actcacggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc 
     2701 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 
     2761 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 
     2821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     2881 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt 
     2941 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     3001 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     3061 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     3121 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     3181 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     3241 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     3301 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     3361 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     3421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     3481 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt 
     3541 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     3601 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 
     3661 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     3721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     3781 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     3841 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 
     3901 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 
     3961 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 
     4021 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 
     4081 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 
     4141 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 
     4201 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 
     4261 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 
     4321 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 
     4381 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 
     4441 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 
     4501 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 
     4561 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 
     4621 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 
     4681 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 
     4741 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     4801 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     4861 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 
     4921 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 
     4981 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 
     5041 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 
     5101 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 
     5161 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     5221 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     5281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     5341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     5401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 
     5461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     5521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     5581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     5641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     5701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     5761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     5821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     5881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     5941 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca 
     6001 tgcat 
//
