LOCUS       pEGFP-N1                6086 bp    DNA     circular SYN 23-MAR-2007
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55762
VERSION     U55762.1  GI:1377911
SOURCE      Cloning vector pEGFP-N1.
  ORGANISM  Cloning vector pEGFP-N1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1), 33-38 (1996)
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|441652552|
COMMENT     VNTDBDATE|441652552|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|LL|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     CDS             3982..4776
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV-F
     misc_feature    2032..2751
                     /vntifkey="21"
                     /label=moxGFP
     misc_feature    877..879
                     /vntifkey="21"
                     /label=T215\glycosylation
     misc_feature    880..882
                     /vntifkey="21"
                     /label=T216\glycosylation
     misc_feature    856..858
                     /vntifkey="21"
                     /label=T208\glycosylation
     misc_feature    841..843
                     /vntifkey="21"
                     /label=T203\glycosylation
     misc_feature    823..825
                     /vntifkey="21"
                     /label=T197\glycosylation
     misc_feature    811..891
                     /vntifkey="21"
                     /label=TAC\stem\region\wt
     misc_feature    625..651
                     /vntifkey="21"
                     /label=ST\Cyto
     misc_feature    652..702
                     /vntifkey="21"
                     /label=ST\TMD
     misc_feature    703..804
                     /vntifkey="21"
                     /label=ST\stem\region
     misc_feature    898..1935
                     /vntifkey="21"
                     /label=ST\Catalytic\domain
     misc_feature    2011..2013
                     /vntifkey="21"
                     /label=T215\glycosylation
     misc_feature    2014..2016
                     /vntifkey="21"
                     /label=T216\glycosylation
     misc_feature    1990..1992
                     /vntifkey="21"
                     /label=T208\glycosylation
     misc_feature    1975..1977
                     /vntifkey="21"
                     /label=T203\glycosylation
     misc_feature    1957..1959
                     /vntifkey="21"
                     /label=T197\glycosylation
     misc_feature    1945..2025
                     /vntifkey="21"
                     /label=TAC\stem\region\wt
BASE COUNT     1525 a      1647 c      1549 g      1365 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatgatt cacaccaacc tgaagaaaaa gttcagctgc 
      661 tgcgtcctgg tctttcttct gtttgcagtc atctgtgtgt ggaaggaaaa gaagaaaggg 
      721 agttactatg attcctttaa attgcaaacc aaggaattcc aggtgttaaa gagtctgggg 
      781 aaattggcca tggggtctga ttccccgcgg ctcgtcacaa caacagattt tcaaatacag 
      841 acagaaatgg ctgcaaccat ggagacgtcc atatttacaa cagagtacca gggatcccag 
      901 tctgtatcct caagcagcac ccaggacccc cacaggggcc gccagaccct cggcagtctc 
      961 agaggcctag ccaaggccaa accagaggcc tccttccagg tgtggaacaa ggacagctct 
     1021 tccaaaaacc ttatccctag gctgcaaaag atctggaaga attacctaag catgaacaag 
     1081 tacaaagtgt cctacaaggg gccaggacca ggcatcaagt tcagtgcaga ggccctgcgc 
     1141 tgccacctcc gggaccatgt gaatgtatcc atggtagagg tcacagattt tcccttcaat 
     1201 acctctgaat gggagggtta tctgcccaag gagagcatta ggaccaaggc tgggccttgg 
     1261 ggcaggtgtg ctgttgtgtc gtcagcggga tctctgaagt cctcccaact aggcagagaa 
     1321 atcgatgatc atgacgcagt cctgaggttt aatggggcac ccacagccaa cttccaacaa 
     1381 gatgtgggca caaaaactac cattcgcctg atgaactctc agttggttac cacagagaag 
     1441 cgcttcctca aagacagttt gtacaatgaa ggaatcctaa ttgtatggga cccatctgta 
     1501 taccactcag atatcccaaa gtggtaccag aatccggatt ataatttctt taacaactac 
     1561 aagacttatc gtaagctgca ccccaatcag cccttttaca tcctcaagcc ccagatgcct 
     1621 tgggagctat gggacattct tcaagaaatc tccccagaag agattcagcc aaacccccca 
     1681 tcctctggga tgcttggtat catcatcatg atgacgctgt gtgaccaggt ggatatttat 
     1741 gagttcctcc catccaagcg caagactgac gtgtgctact actaccagaa gttcttcgat 
     1801 agtgcctgca cgatgggtgc ctaccacccg ctgctctatg agaagaattt ggtgaagcat 
     1861 ctcaaccagg gcacagatga ggacatctac ctgcttggaa aagccacact gcctggcttc 
     1921 cggaccattc actgcgcacc ggtcctcgtc acaacaacag attttcaaat acagacagaa 
     1981 atggctgcaa ccatggagac gtccatattt acaacagagt accaggccac catggtgtcc 
     2041 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 
     2101 aacggccaca agttctccgt gcggggcgag ggcgagggcg atgccaccaa cggcaagctg 
     2161 accctgaagt tcatcagcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 
     2221 accctgacct acggcgtgca gagcttctcc cgctaccccg accacatgaa gcgccacgac 
     2281 ttcttcaaga gcgccatgcc cgaaggctac gtccaggagc gcaccatctc cttcaaggac 
     2341 gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 
     2401 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 
     2461 tacaacttca actcccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag 
     2521 gccaacttca agatccgcca caacgtggag gacggctccg tgcagctcgc cgaccactac 
     2581 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgtcc 
     2641 acccagtcca agctgtccaa agaccccaac gagaagcgcg atcacatggt ccttctggaa 
     2701 ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagta aagcggccgc 
     2761 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 
     2821 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 
     2881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     2941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 
     3001 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 
     3061 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 
     3121 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 
     3181 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 
     3241 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 
     3301 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 
     3361 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 
     3421 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 
     3481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
     3541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 
     3601 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 
     3661 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 
     3721 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 
     3781 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
     3841 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
     3901 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 
     3961 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 
     4021 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 
     4081 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 
     4141 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 
     4201 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 
     4261 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 
     4321 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 
     4381 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 
     4441 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 
     4501 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 
     4561 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 
     4621 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 
     4681 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 
     4741 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 
     4801 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 
     4861 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 
     4921 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 
     4981 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 
     5041 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 
     5101 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 
     5161 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 
     5221 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 
     5281 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 
     5341 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
     5401 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
     5461 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
     5521 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
     5581 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
     5641 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
     5701 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
     5761 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
     5821 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
     5881 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
     5941 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
     6001 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 
     6061 tggataaccg tattaccgcc atgcat 
//
