LOCUS       1637_pA_CS-KDEL_        6716 bp    DNA     circular      4-FEB-2015
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
            SerialCloner_Type=DNA
            SerialCloner_Comments=Ligation of : 1535_pA_CS-STIM1-NN_SBP-EGFP-Ecadherin.xdna [8
            121  nt] : (NotI[3519] / RsrII[1556]) to  [19  nt] : (Uncut 
            5'[0] / Uncut 3'[19])
            SerialCloner_Ends=0,0,,0,
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|724760008|
COMMENT     VNTDBDATE|724760008|
COMMENT     LSOWNER|
COMMENT     VNTNAME|1637_pA_CS-KDEL_SBP-EGFP-CD8A-Furin.xdna|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                            5 "ACCGGT" "TGGCCA") 
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COMMENT                            6 2 "GGCCGGCC" "CCGGCCGG") 
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COMMENT                            5 1 "GGTACC" "CCATGG") 
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COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            2 4 "CATATG" "GTATAC") 
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COMMENT                            5 "GCTAGC" "CGATCG") 
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COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                            5 1 "CTGCAG" "GACGTC") 
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COMMENT                            5 1 "GAGCTC" "CTCGAG") 
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COMMENT                            1 5 "GTCGAC" "CAGCTG") 
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COMMENT                            3 3 "AGTACT" "TCATGA") 
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COMMENT                            3 3 "CCCGGG" "GGGCCC") 
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COMMENT                            1 5 "TCTAGA" "AGATCT") 
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COMMENT                            1 5 "CTCGAG" "GAGCTC") 
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COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     misc_feature    1563..1574
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="KDEL" \
                     /vntifkey="21"
                     /label=KDEL
     primer_bind     complement(4555..4575)
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /vntifkey="28"
                     /label=M13-rev
     primer_bind     863..882
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /vntifkey="28"
                     /label=T7
     rep_origin      complement(4943..5571)
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /vntifkey="33"
                     /label=ColE1\origin
     misc_binding    complement(4581..4603)
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /vntifkey="20"
                     /label=LacO
     CDS             complement(5723..6382)
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /vntifkey="4"
                     /label=AmpR
     CDS             2716..3429
                     /SerialCloner_Color=&h32CD32
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /vntifkey="4"
                     /label=EGFP
     misc_feature    1077..1553
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /vntifkey="21"
                     /label=Core\Streptavidin
     CDS             2593..2703
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     /vntifkey="4"
                     /label=SBP\tag
     misc_feature    2533..2586
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \
                     /SerialCloner_Desc=translation="MYRMQLLSCIALSLALVT" \
                     /vntifkey="21"
                     /label=signal\peptide
     misc_feature    4099..4155
                     /vntifkey="21"
                     /label=TMD\of\Tac
     misc_feature    4156..4194
                     /vntifkey="21"
                     /label=Cytosolic\tail\of\Tac
     misc_feature    3442..4098
                     /vntifkey="21"
                     /label=Extracellular\domain\of\Tac
BASE COUNT     1709 a      1777 c      1690 g      1540 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat cgatatctgc ggcctagcta gcgcttaagg cctgttaacc ggtatggatg 
      961 tatgcgtccg tcttgccctg tggctcctct ggggactcct cctgcaccag ggccagagcc 
     1021 tcagccatag tcacagtgag aaggcgacag gaaccagctc gggcgccggt ttaaacgacc 
     1081 ctagcaaaga ctcaaaagct caggtgtccg ctgccgaggc tggcattact ggaacatggt 
     1141 acaatcagct cgggagcacc tttattgtga ctgctggagc cgatggagcc ctcaccggaa 
     1201 catacgaatc tgctgtggga aacgccgaat cacggtacgt cctcactggc cgatacgata 
     1261 gtgcccctgc caccgacgga tctgggactg ccctgggatg gactgtcgct tggaaaaaca 
     1321 actaccggaa tgctcattct gccacaacat ggagtggaca gtacgtggga ggcgctgagg 
     1381 ctagaatcaa tacacagtgg ctgctcacat ctggcacaac cgaggcaaat gcttggaaat 
     1441 ccaccctggt gggacatgac acattcacca aagtgaaacc ctccgccgct tcaatcgatg 
     1501 ccgccaaaaa agccggagtc aacaacggca atcctctgga tgccgtccag cagctcggac 
     1561 cgaaagatga actgtgagcg gccgcataga taactgatcc agtgtgctgg aattaattcg 
     1621 ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct 
     1681 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg 
     1741 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc 
     1801 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg 
     1861 cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg 
     1921 cggccaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga 
     1981 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 
     2041 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 
     2101 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 
     2161 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 
     2221 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 
     2281 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag 
     2341 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 
     2401 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc 
     2461 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc 
     2521 gggaggcgcg ccatgtacag gatgcaactc ctgtcttgca ttgcactaag tcttgcactt 
     2581 gtcacgaatt ccgacgagaa gaccactggt tggcgaggtg gacacgttgt tgaaggactg 
     2641 gctggggaac ttgaacaact tcgtgcacga ctggagcatc acccacaagg tcaacgtgaa 
     2701 ccacctgcag gtatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
     2761 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
     2821 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
     2881 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
     2941 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
     3001 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
     3061 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     3121 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     3181 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     3241 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     3301 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     3361 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     3421 ctgtacaagg gccggccaga cgagctctgt gacgatgacc cgccagagat cccacacgcc 
     3481 acattcaaag ccatggccta caaggaagga accatgttga actgtgaatg caagagaggt 
     3541 ttccgcagaa taaaaagcgg gtcactctat atgctctgta caggaaactc tagccactcg 
     3601 tcctgggaca accaatgtca atgcacaagc tctgccactc ggaacacaac gaaacaagtg 
     3661 acacctcaac ctgaagaaca gaaagaaagg aaaaccacag aaatgcaaag tccaatgcag 
     3721 ccagtggacc aagcgagcct tccaggtcac tgcagggaac ctccaccatg ggaaaatgaa 
     3781 gccacagaga gaatttatca tttcgtggtg gggcagatgg tttattatca gtgcgtccag 
     3841 ggatacaggg ctctacacag aggtcctgct gagagcgtct gcaaaatgac ccacgggaag 
     3901 acaaggtgga cccagcccca gctcatatgc acaggtgaaa tggagaccag tcagtttcca 
     3961 ggtgaagaga agcctcaggc aagccccgaa ggccgtcctg agagtgagac ttcctgcctc 
     4021 gtcacaacaa cagattttca aatacagaca gaaatggctg caaccatgga gacgtccata 
     4081 tttacaacag agtaccaggt agcagtggcc ggctgtgttt tcctgctgat cagcgtcctc 
     4141 ctcctgagtg ggctcacctg gcagcggaga cagaggaaga gtagaagaac aatctagctc 
     4201 gagggccaat taattaactc tagataactg atcataatca gccataccac atttgtagag 
     4261 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 
     4321 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     4381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     4441 ctcatcaatg tatcttaacg cgtcgagtgc attctagttg tggtttgtcc aaactcatca 
     4501 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 
     4561 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     4621 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     4681 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     4741 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     4801 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     4861 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     4921 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     4981 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     5041 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     5101 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 
     5161 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     5221 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     5281 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     5341 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     5401 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     5461 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     5521 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     5581 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     5641 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     5701 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     5761 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     5821 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     5881 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     5941 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     6001 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     6061 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     6121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     6181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     6241 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     6301 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     6361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     6421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     6481 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     6541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     6601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     6661 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//
