LOCUS       Exported                8640 bp    DNA     circular SYN 13-Nov-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ir8a Plasmid 1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 13, 2017 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            complement(146..416)
                     /note="lacZalpha"
     misc_feature    417..2125
                     /label=left homology arm
     misc_feature    1996..2015
                     /label=Ir8a sgRNA 1
                     /note="modified to prevent recutting"
     misc_feature    2123..2125
                     /label=last codon of Ir8a
     misc_feature    2126..2182
                     /note="T2A"
     misc_feature    2183..3238
                     /note="QF2"
     misc_feature    3243..3499
                     /note="SV40 Term"
     misc_feature    3500..3745
                     /note="3xP3"
     misc_feature    3775..4452
                     /note="dsRed"
     misc_feature    4467..4694
                     /note="SV40"
     misc_feature    4695..6376
                     /label=right homology arm
     misc_feature    4731..4747
                     /label=Ir8a sgRNA 2
                     /note="modified to prevent recutting"
     -10_signal      complement(6468..6473)
                     /note="Plac promoter"
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(6492..6497)
                     /note="Plac promoter"
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     protein_bind    complement(6517..6529)
                     /note="CAP protein binding site"
     rep_origin      complement(6821..7409)
                     /direction=LEFT
                     /note="pMB1 origin of replication"
                     /note="pMB1 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(6821..7373)
                     /note="RNAII transcript"
                     /note="RNAII transcript (complementary strand)"
     -35_signal      7227..7232
                     /note="RNAI promoter"
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      7249..7254
                     /note="RNAI promoter"
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        7263..7370
                     /note="RNAI transcript"
     -10_signal      complement(7383..7388)
                     /note="RNAII promoter"
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(7404..7409)
                     /note="RNAII promoter"
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     gene            complement(7580..8440)
                     /note="bla"
     CDS             complement(7580..8440)
                     /codon_start=1
                     /note="beta-lactamase"
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    7720..7725
                     /note="BsaI"
     sig_peptide     complement(8372..8440)
                     /note="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     -10_signal      complement(8484..8489)
                     /note="bla promoter"
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(8505..8510)
                     /note="bla promoter"
                     /note="bla promoter (counter-clockwise); TTCAAA"
BASE COUNT      2285 A      2163 C      2006 G      2186 T         0 OTHER
ORIGIN      ?
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGTCAG
      421 AATCTTCTCA AGATTCTCAT CAGAATCTTC TCAAGATTCT CATCAGAATC TTCTCAAGAT
      481 TCTCATCAGA ATCCTCTCAG GATTCTCATC AGAATCCTCT CAGGATTCTC ATCAGAATCC
      541 TCTCAGGATT CTCATCAGAA TCCTCTCAAG ATTCTCATCA GAATCTTCTC ACGAATCTCA
      601 TTAGAATCCT CTCAGGATTA TCATTAGAAT CCTCTCAAGA TTCTCATTAG AATCCTCCCA
      661 GGATTCTCAT CAGAATCCTC TCAGGAATCT CATCACACTG CTCTAAAGAT ACTCGTCAGA
      721 ATCCTCTTAG GATCCTCATC AGAATCCTCT CAGGACTCTC ATCAGAATCC TCTCAGCATT
      781 CTCATCAGAA TCCCCTCAGC ATTCTCATCG AAATCCTTCA GGATGCTCAT CAGAATCCTC
      841 TAAAGATTCT GATGAGCTTC TTTAGAGGAT TCTCATCAGA ATCCTCTGAG GATTCTCATT
      901 AGAATCCTCT CAGCATTCTC TAAGGATTTT TTTCAGAATC CTCTCAGGAT TTTCATTAGA
      961 ATCCTGTCAG AATTTTCATT AGAATCCTCT CAGGATTCTT ATCAGAACCC TCTCAGGATT
     1021 CAGTGAAGAC TTTAACCTTT AATTCTGTTT CAGAGACTTC ACTCTCAAGG AAAGCTTCTG
     1081 GTTTGCCTTA ACTTCATTCA CTCCACAAGG CGGTGGAGAA GCCCCAAAGG CACTTTCCGG
     1141 ACGTACCCTG GTGGCGGCAT ACTGGTTGTT CGTCGTACTA ATGTTGGCTA CATTTACCGC
     1201 GAACCTGGCT GCGTTTCTTA CCGTTGAACG TATGCAGACT CCGGTACAAT CCTTGGAGCA
     1261 GCTGTCCCGT CAAAGTCGCA TCAAATACAC AGTTGTGAAG GACTCCGACA CGCATGATTA
     1321 CTTCAAGAAC ATGAAGAACG CCGAAGACGT ACTGTACCGA ATGTGGCGTA ATCTGACGCT
     1381 TTCGAGTGGA AACGACCAGA CGCAGTATCG CGTTTGGGAT TACCCGATCA AGGAACAGTA
     1441 TACCAATATC TTGGCTGCCA TAGAGTCAGC GAATCCAGTC CAGTCGGCTG AAGTTGGATT
     1501 CCAACGAGTC AACGAGGGTC TAGAAGCGGA TTTTGCATTC GTTCACGATT CAGCGGAGAT
     1561 CAAGTACGAA ATATCACGCA ACTGTAATCT CACGGAAGTT GGGGAAGTTT TCGGTGAACA
     1621 GCCGTATGCG ATCGCAGTTC AACAGGGAAG TCATCTGCAA GACGAGTTGA GTTATTACAT
     1681 TCTGGAACTG CAAAAGGAAC GCTACTTCGA ATCGCTGACG GCAAAGTTTT GGAACAATTC
     1741 GGTACGCGGA ATTTGTCCCA ATACGGACGA CAGCGAAGGA ATAACACTGG AAAGCTTGGG
     1801 TGGAGTGTTC ATAGCGACTC TTGTGGGACT AGCCCTGGCA ATGGTAACTT TGGCTGGGGA
     1861 AGTGTTGTAT TACCGCCGTA AGGATCAGAA GGGCAAGCTG GTGCAGGTTG AGCCGATGGA
     1921 TGTAGGAAAA AAGAAAGTAG TAAAGGATAA GCATTCGCTT ACGTTCAAAA ATTTGCTGAA
     1981 CACTGTAGAA ATGAAGAACA ATCTCAACAA GGTTGTTGAG AAAAAGATCC CAAAGGAGAT
     2041 CACCATTGGA AATAAGTTTG TGCCAGCATC GGAGAAGCAA CAGAAGATCA GCTACATATC
     2101 GGTATTTCCT AAGAATCCGA TGAATGGAGA GGGCAGAGGA AGTCTTCTAA CATGCGGTGA
     2161 CGTGGAGGAG AATCCCGGCC CTATGCCACC CAAGCGCAAA ACGCTTAACG CTGCGGCTGA
     2221 GGCTAACGCT CATGCCGACG GACACGCCGA CGGAAACGCC GACGGACACG TGGCCAATAC
     2281 GGCCGCGTCC TCGAATAATG CGAGGTTCGC TGATCTCACT AACATCGATA CTCCGGGTCT
     2341 GGGACCCACA ACTACGACCC TGCTCGTGGA ACCAGCACGC TCAAAGCGTC AACGAGTGTC
     2401 CCGCGCATGC GACCAGTGCC GTGCAGCCCG AGAGAAATGC GACGGAATAC AGCCTGCGTG
     2461 TTTCCCGTGC GTTTCCCAGG GAAGGTCCTG CACTTATCAG GCTTCGCCGA AAAAGAGGGG
     2521 AGTTCAAACC GGTTATATTC GTACGCTGGA GCTCGCCCTC GCCTGGATGT TTGAAAATGT
     2581 CGCGCGTTCC GAAGATGCCT TGCATAACCT CCTCGTCCGT GACGCCGGAC AAGGATCAGC
     2641 TCTGCTCGTT GGTAAAGATT CGCCGGCTGC CGAGCGACTC CATGCCCGTT GGGCTACTAG
     2701 CCGTGTCAAT AAGAGCATTA CCCGCCTCCT CCGTCAGTTG GAGCTCCCTC CTACCGCCAC
     2761 GGCTACGGCC TCGATAATGC CGCACGTGAT GGAGCAGCCT CTCAGTACCA GCATTAACCC
     2821 CGTCAACGAC CGCTTCAACG GTATTCCCAA CCCCACTCCG TATAACTCCG ATGCAGCTCT
     2881 CGATGCTATC ACTCAGACCA ACGATTATGG AAGCGTAAAT ACACATGGTA TCCTCTCTAC
     2941 TTACCCGCCA CCGGCTACGC ACCTTAATGA AGCTTCCGTC GCTCTCGCTC CCGGTGGCGC
     3001 CCCCCCCCGA CCGCCTCCTC CGTATGTTGA CAGCACGACC AATCACCCGC CGTACCACTC
     3061 GAATCTGGTT CCAATGGCGA ACTTTGGTTA CTCGACCGTT GATTACGATG CCATGGTTGA
     3121 CGATTTGGCT AGCATTGAAT ACACGGACGC TGTGGATGTC GACCCACAGT TTATGACCAA
     3181 TCTGGGATTC GTTCCTGGAT GTAACTTCTC CGACATTAAT ACATACGAAC AGTGATGACG
     3241 TCTATCGATA CCGTCGACTA AAGCCAAATA GAAAATTATT CAGTTCCTGG CTTAAGTTTT
     3301 TAAAAGTGAT ATTATTTATT TGGTTGTAAC CAACCAAAAG AATGTAAATA ACTAATACAT
     3361 AATTATGTTA GTTTTAAGTT AGCAACAAAT TGATTTTAGC TATATTAGCT ACTTGGTTAA
     3421 TAAATAGAAT ATATTTATTT AAAGATAATT GCGTTTTTAT TGTCAGGGAG TGAGTTTGCT
     3481 TAAAAACTCG TTTAGATCCA ATTCGAGCTC GCCCGGGGAT CTAATTCAAT TAGAGACTAA
     3541 TTCAATTAGA GCTAATTCAA TTAGGATCCA AGCTTATCGA TTTCGAACCC TCGACCGCCG
     3601 GAGTATAAAT AGAGGCGCTT CGTCTACGGA GCGACAATTC AATTCAAACA AGCAAAGTGA
     3661 ACACGTCGCT AAGCGAAAGC TAAGCAAATA AACAAGCGCA GCTGAACAAG CTAAACAATC
     3721 GGGGTACCGC TAGAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGTCGC CACCATGGCC
     3781 TCCTCCGAGA ACGTCATCAC CGAGTTCATG CGCTTCAAGG TGCGCATGGA GGGCACCGTG
     3841 AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCCACAAC
     3901 ACCGTGAAGC TGAAGGTGAC CAAGGGCGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC
     3961 CCCCAGTTCC AGTACGGCTC CAAGGTGTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC
     4021 AAGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC
     4081 GGCGTGGCGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCTGCTTCAT CTACAAGGTG
     4141 AAGTTCATCG GCGTGAACTT CCCCTCCGAC GGCCCCGTGA TGCAGAAGAA GACCATGGGC
     4201 TGGGAGGCCT CCACCGAGCG CCTGTACCCC CGCGACGGCG TGCTGAAGGG CGAGACCCAC
     4261 AAGGCCCTGA AGCTGAAGGA CGGCGGCCAC TACCTGGTGG AGTTCAAGTC CATCTACATG
     4321 GCCAAGAAGC CCGTGCAGCT GCCCGGCTAC TACTACGTGG ACGCCAAGCT GGACATCACC
     4381 TCCCACAACG AGGACTACAC CATCGTGGAG CAGTACGAGC GCACCGAGGG CCGCCACCAC
     4441 CTGTTCCTGT AGCGGCCGCG ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT
     4501 TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA
     4561 ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
     4621 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
     4681 ATCAATGTAT CTTAAAATGA ATTTGCAATT AAGGGTCATT TTTAAATTAC GTCACGCTTG
     4741 TTGTACAAGT TCATCGAAAC GTGACGACCC ATACACAATT TTCGATGTTT CCATATGTGT
     4801 AAAATTTTGC ATGACGAGAT TTATGGACGT TTCTTAATTG AATTATGGGC ATCACAGATA
     4861 AGTATACTAA AAAGACGAAA TTGATAAATA AATAAATTAA TAAGAGTTTG TTGAAACATA
     4921 GCCTATGGTT TATTTTCGAA GATTTGAACA GAAAAACCCC GACTAAGTCC GTTGAGACTC
     4981 GTATGTTTGT CTTTATTTCG TGCCTTTTCG TTTTCAGTTC CTTGTTTTTC GCTCGTTTGC
     5041 TGTGTGTGTG TTGGGACACA ACAGTAGTCA CCTTAAATAC TATTTGCAGT AATTTTCTTG
     5101 ATTTTCCATC CGTTTGCCGT AATTCTTACG GTTTTATGAA TTTATTACCA TCCCTTGACT
     5161 TGACTTGGCT AGACAGTACA ATATACGTGT GAACAACACA TACAATTTTA CTATAGGTTT
     5221 GAAAAGTTGT TTGCCCATTA GAGGGATTGG AACCACATCC AATCTCTAGA AAATTCTTAT
     5281 TCTGTTTTTT GGAGGTTGAA ATTTAGTGCA CTAACGAAAA GTATAAAGGC CATCAATAAA
     5341 AAAAAGCTTA GAGGGAAACG ATTGGCTTCA CGCAGGTAAT CAAGAGAAGT ACATTTATTC
     5401 CACAATCCAC AATTGCTACT CAGTGTACAT CAAACTAAAC GAATCATTAC ATGTTATTGC
     5461 ATTTTCAGCA GCATCTAGGA GTGATGACTT CTTCTCTACA AAGGGTATTA TTCCATCACC
     5521 TACATTCCGC ATGCGCCTTG TGTGGCTTTA CTCTTTGTTG AGGACAATCC TATCGCGGTG
     5581 TACTATGCAG TTCTTCGCAC GCCGCTGGGC TCGCACTAGG CAGCATAACT GTCTATGTGT
     5641 GATGCTGGAG AAGCGCATCA TTGAATGATT TTAATGTGGT TGTCATCGTA TTGTATCTGT
     5701 GTACTTCAGT TAGTTTGTTT ACGTCCCGTA TCGTTTCGTA CCTTTTATCG TGTCGTAATA
     5761 AAGTATGTTT TAAATTGTTA CGTGCGCGTT TAATTCCCTG ATGTAGGGAC GAGTTTAAAG
     5821 AACAACCATC GGGGTATTTC CACGGTACAA AAGTGGCGAC GAGAATTCGC GAGTGAGGGT
     5881 AGTTTTTCCG TCGATAAAAA CTCACCCAAC ATTTCGTCAC CCGCGCGGAA GAAAAGAAAG
     5941 TGGAGTGCGA TGGCTGACGG TGATAGTGAT CGAAACGGGC AAAATGGTGT TGACCCCCCG
     6001 GGAGTGATAA ATCGGCAGCA ATTAAACCAG CCGATTATCC CCGCGCCGGC GGCTCACCAG
     6061 CAGCAGCAGC AGCAGCAAAT TTTCGTCCCC GTTGTGACTA CACACCAGCA GCAGCAGCCA
     6121 TTCGTTCCGG TTTCTTCAGC TCCACAACAG CAGTTCTCAA CCTTCCAATC CCACCAAACA
     6181 CCGCAAGCCC AAGTAAGTAC CGATATGCTC ATGCAAATTA TGCATATGAT GCAGCAATCG
     6241 ATGACCCAGA GTCAGCAGCA GCACCAACAG CTTATGGGGC AAATTATTGC GCAGCAGCAG
     6301 CAGTTCCTAA GTAGCGTCGC GTCGTCAATA AATGTTCAAG TGCCACCCAA TCCGGAACAA
     6361 ATCCTTGACT CCTTGGTCTA GAGTCGACCT GCAGGCATGC AAGCTTGGCG TAATCATGGT
     6421 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG
     6481 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT
     6541 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG
     6601 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG
     6661 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
     6721 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
     6781 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
     6841 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
     6901 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
     6961 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
     7021 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
     7081 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
     7141 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
     7201 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
     7261 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
     7321 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
     7381 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
     7441 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA
     7501 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG
     7561 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
     7621 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG
     7681 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC
     7741 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA
     7801 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC
     7861 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT
     7921 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC
     7981 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT
     8041 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC
     8101 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT
     8161 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA
     8221 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA
     8281 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG
     8341 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA
     8401 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT
     8461 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
     8521 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG
     8581 AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC
//
