LOCUS       Exported                8623 bp    DNA     circular SYN 21-Oct-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 21, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..414
                     /label=Cloning vector pUC19
     source          join(6360..8623,1)
                     /label=Cloning vector pUC19
     misc_feature    1..59
                     /label=BsmBI
                     /note="BsmBI"
     gene            complement(146..416)
                     /label=lacZalpha
     CDS             complement(146..416)
                     /codon_start=1
                     /label=beta-galactosidase alpha fragment
                     /translation="PGTELEFTGRRFTTS*LGKPWRYPT*SPCSTSPFRQLA**RRGPH
                     RSPFPTVAQPEWRMAPDAVFSPYASVRYFTPHMVHSQYNLL*CRI"
     misc_feature    417..1626
                     /label=Ir76b left homology arm
     misc_feature    1624..1626
                     /label=last codon of Ir76b
     misc_feature    1627..1683
                     /label=T2A
                     /note="T2A"
     misc_feature    1684..2739
                     /label=QF2
                     /note="QF2"
     misc_feature    2744..3000
                     /label=SV40 Term
                     /note="SV40 Term"
     misc_feature    3001..3246
                     /label=3xP3
                     /note="3xP3"
     misc_feature    3276..3953
                     /label=dsRed
                     /note="dsRed"
     misc_feature    3968..4195
                     /label=SV40
                     /note="SV40"
     misc_feature    4196..6388
                     /label=Ir76b right homology arm
                     /note="R Arm"
     misc_feature    4503..4504
                     /label=Ir76b sgRNA 4 (removed from donor)
                     /note="removed from donor to prevent recutting"
     -10_signal      complement(6451..6456)
                     /label=Plac promoter
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(6475..6480)
                     /label=Plac promoter
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     protein_bind    complement(6500..6512)
                     /label=CAP protein binding site
                     /note="CAP protein binding site"
     rep_origin      complement(6804..7392)
                     /direction=LEFT
                     /label=pMB1 origin of replication
                     /note="pMB1 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(6804..7356)
                     /label=RNAII transcript
                     /note="RNAII transcript (complementary strand)"
     -35_signal      7210..7215
                     /label=RNAI promoter
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      7232..7237
                     /label=RNAI promoter
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        7246..7353
                     /label=RNAI transcript
                     /note="RNAI transcript"
     -10_signal      complement(7366..7371)
                     /label=RNAII promoter
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(7387..7392)
                     /label=RNAII promoter
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     gene            complement(7563..8423)
                     /label=bla
     CDS             complement(7563..8423)
                     /codon_start=1
                     /label=beta-lactamase
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    7703..7708
                     /label=BsaI
                     /note="BsaI"
     sig_peptide     complement(8355..8423)
                     /label=bla
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     -10_signal      complement(8467..8472)
                     /label=bla promoter
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(8488..8493)
                     /label=bla promoter
                     /note="bla promoter (counter-clockwise); TTCAAA"
BASE COUNT      2319 A      2099 C      1999 G      2206 T         0 OTHER
ORIGIN      ?
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGTTTC
      421 TACTGTTACG AATAGAAAAG CGGGGTGTCT GCCTTTCTTT ATTGAAATCA AAAATCAACA
      481 ACTCGCCGTC TATAGTGGTT ACACGTGTTC GCCATTGGCG TAGACCAAAA ATAAATAGAA
      541 TTTTCGATTG GGTCTATCAC ACACCCATGA GCAGTTAGAC AAAAAAACTT ATTGGTGAAG
      601 GTATAAATCA ATGCACGTCA GCCTAGACAA CCTTTCCGAA CCTTATCCCC TGCCCCCTTC
      661 CTAACAGTTG CTGTTTTCAT AGAGCGACGA GACCCTGAAC TCGTTGAGTT TTTTGGTGGA
      721 CAAACACTTG GTGGAGTTCA CAACCAACCT GAACGATAGC GACATCCTGA TGACAACGGT
      781 GTCCAAGAAA AACTACGTCT ACGTACGGGA TCGACCGGCC ATCGAACATC TGGTTTACAA
      841 AGACTACCTC GTTCGGCGCA AGGTGAACCC CATCAACGAA CGTAGCCATT GTCCGTTTGC
      901 AACGGCTACA ACACCTTTCC TGAAACGGGT TCGCGCCTTT GCCTATCCCA ATACCACACG
      961 CTGGAACACG CTATTTGATC CACAGTAAGT TCTACTAAAA GTATAACAAA GACGCCATGT
     1021 ATAAACCATT TCTTCGTTCT ACGCAGACTA CTCAACATGG TCGAAGGAGG CTTGGTGAAA
     1081 TTCAAGCTTC TGGAGAATCT ACCCAAGGCC GAGATCTGTC CGCAGAACTT AGGAGGCACT
     1141 GAGCGTCAGC TGAAGAATCG GGACTTGGTC ATGACATACT TGGTGATGTT GACCGGCTTT
     1201 GCCACGGCAA TTGCTGTATT CGTTTCGGAG GTAACTACAT CTGCTTGATC ACAGCTCTAG
     1261 TAGGCCGTTA ACAATATTCT TCTTGTTGTC TTCCTATAGA TGTTCTTCCG CATCCTGAAC
     1321 GAGCGCAAAC TGAACAAAGA ACAGTCGGAA GCTGCCCGGA ACCGTGGCAC CGATGGAGTT
     1381 ACCTATGTGA ATGCAAAGGC CATGGGTTAT GGCGATTCAC CGCCGCCGCC GTATGCGGAG
     1441 ATTTTCAGCC GCCATGCTAG CGGTGGAAGC ATGTTTGGAA GCAGTATTGA CAGTGGTAGC
     1501 GGTGCATCAC GGAGGATGAT CAACGGCAGG GATTACATGG TCGTCAAGGA GCGAAATGGG
     1561 ATCGGTTCGC AGCTCATACC AATTCGGGCG CCGTCAGCTG CAATTTTTCA GTACACTTAT
     1621 ACGAATGGAG AGGGCAGAGG AAGTCTTCTA ACATGCGGTG ACGTGGAGGA GAATCCCGGC
     1681 CCTATGCCAC CCAAGCGCAA AACGCTTAAC GCTGCGGCTG AGGCTAACGC TCATGCCGAC
     1741 GGACACGCCG ACGGAAACGC CGACGGACAC GTGGCCAATA CGGCCGCGTC CTCGAATAAT
     1801 GCGAGGTTCG CTGATCTCAC TAACATCGAT ACTCCGGGTC TGGGACCCAC AACTACGACC
     1861 CTGCTCGTGG AACCAGCACG CTCAAAGCGT CAACGAGTGT CCCGCGCATG CGACCAGTGC
     1921 CGTGCAGCCC GAGAGAAATG CGACGGAATA CAGCCTGCGT GTTTCCCGTG CGTTTCCCAG
     1981 GGAAGGTCCT GCACTTATCA GGCTTCGCCG AAAAAGAGGG GAGTTCAAAC CGGTTATATT
     2041 CGTACGCTGG AGCTCGCCCT CGCCTGGATG TTTGAAAATG TCGCGCGTTC CGAAGATGCC
     2101 TTGCATAACC TCCTCGTCCG TGACGCCGGA CAAGGATCAG CTCTGCTCGT TGGTAAAGAT
     2161 TCGCCGGCTG CCGAGCGACT CCATGCCCGT TGGGCTACTA GCCGTGTCAA TAAGAGCATT
     2221 ACCCGCCTCC TCCGTCAGTT GGAGCTCCCT CCTACCGCCA CGGCTACGGC CTCGATAATG
     2281 CCGCACGTGA TGGAGCAGCC TCTCAGTACC AGCATTAACC CCGTCAACGA CCGCTTCAAC
     2341 GGTATTCCCA ACCCCACTCC GTATAACTCC GATGCAGCTC TCGATGCTAT CACTCAGACC
     2401 AACGATTATG GAAGCGTAAA TACACATGGT ATCCTCTCTA CTTACCCGCC ACCGGCTACG
     2461 CACCTTAATG AAGCTTCCGT CGCTCTCGCT CCCGGTGGCG CCCCCCCCCG ACCGCCTCCT
     2521 CCGTATGTTG ACAGCACGAC CAATCACCCG CCGTACCACT CGAATCTGGT TCCAATGGCG
     2581 AACTTTGGTT ACTCGACCGT TGATTACGAT GCCATGGTTG ACGATTTGGC TAGCATTGAA
     2641 TACACGGACG CTGTGGATGT CGACCCACAG TTTATGACCA ATCTGGGATT CGTTCCTGGA
     2701 TGTAACTTCT CCGACATTAA TACATACGAA CAGTGATGAC GTCTATCGAT ACCGTCGACT
     2761 AAAGCCAAAT AGAAAATTAT TCAGTTCCTG GCTTAAGTTT TTAAAAGTGA TATTATTTAT
     2821 TTGGTTGTAA CCAACCAAAA GAATGTAAAT AACTAATACA TAATTATGTT AGTTTTAAGT
     2881 TAGCAACAAA TTGATTTTAG CTATATTAGC TACTTGGTTA ATAAATAGAA TATATTTATT
     2941 TAAAGATAAT TGCGTTTTTA TTGTCAGGGA GTGAGTTTGC TTAAAAACTC GTTTAGATCC
     3001 AATTCGAGCT CGCCCGGGGA TCTAATTCAA TTAGAGACTA ATTCAATTAG AGCTAATTCA
     3061 ATTAGGATCC AAGCTTATCG ATTTCGAACC CTCGACCGCC GGAGTATAAA TAGAGGCGCT
     3121 TCGTCTACGG AGCGACAATT CAATTCAAAC AAGCAAAGTG AACACGTCGC TAAGCGAAAG
     3181 CTAAGCAAAT AAACAAGCGC AGCTGAACAA GCTAAACAAT CGGGGTACCG CTAGAGTCGA
     3241 CGGTACCGCG GGCCCGGGAT CCACCGGTCG CCACCATGGC CTCCTCCGAG AACGTCATCA
     3301 CCGAGTTCAT GCGCTTCAAG GTGCGCATGG AGGGCACCGT GAACGGCCAC GAGTTCGAGA
     3361 TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCCACAA CACCGTGAAG CTGAAGGTGA
     3421 CCAAGGGCGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCCCAGTTC CAGTACGGCT
     3481 CCAAGGTGTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CAAGAAGCTG TCCTTCCCCG
     3541 AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGCG ACCGTGACCC
     3601 AGGACTCCTC CCTGCAGGAC GGCTGCTTCA TCTACAAGGT GAAGTTCATC GGCGTGAACT
     3661 TCCCCTCCGA CGGCCCCGTG ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCACCGAGC
     3721 GCCTGTACCC CCGCGACGGC GTGCTGAAGG GCGAGACCCA CAAGGCCCTG AAGCTGAAGG
     3781 ACGGCGGCCA CTACCTGGTG GAGTTCAAGT CCATCTACAT GGCCAAGAAG CCCGTGCAGC
     3841 TGCCCGGCTA CTACTACGTG GACGCCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA
     3901 CCATCGTGGA GCAGTACGAG CGCACCGAGG GCCGCCACCA CCTGTTCCTG TAGCGGCCGC
     3961 GACTCTAGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC
     4021 TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT
     4081 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG
     4141 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAGGTGA
     4201 CTTTGGAAAG TAGCCCCACT TTTTTGGTAT AGGTATTGTT CAGCATCAAT CATAAATAGT
     4261 TAACCTTCAA GAGTCGCTGT TACATGACTC GATATCATGT AACGGTTGTC GATGGAAAGT
     4321 ATGCAAAGTG CTGTTTGTCT GTTTTAGAGT TAAAATTTCA TAGTTACTAT GATGGTCCTC
     4381 TCTCAAATAA GTTTCCAAAA CATTTATATA GATCTAGAGT TTTAGTAAAT GTAGATTTTC
     4441 GTTATCTATC CAGACCAGTA CCGGAGATTT CATTTTAAAT CTTTTTATTT GGCATAGCAC
     4501 ACTAACGAAA AATTGCAGTG ACTTAGGGAC CATTGTGCAT ACGAATTCAA ATACAGTGAA
     4561 CTCTCTTTCA ATCGGTATTG AAGGGACCAT CGAGTTAGGG AGGTATCGAG ATGCAGAATA
     4621 CATATTTTTC AGTTTTCAGT TTCAATTTTA GCTTTAAAAC ATTAATTTTA AAAAAGCGTT
     4681 CGAAATAATA GTTTTAACGT TAAAAATTAA AGAATACTAA GCAACACTTT TTGAACAATC
     4741 TTGAAAAACT TCAGATTGTT TCATCCTTAA ATGGGTTGTC AACCTTAATT TTACAATGAT
     4801 CATAGTTAAA ATTTTTGAAA TCTATTAACA TTCGGAGGGT GTATGAAACT TTTCATGTAA
     4861 ACTTCGAGTT AGATAGATAT AAAGTTGCAG GAAACTAGAA CAGATTACAT AGATGTGCTT
     4921 ATTCTGCGCG ACGTGTGAGT CGAGACGAGT CGCTCTCACC GCGAGTGACG TGAGACGACT
     4981 AATGTTAATA AAATGGGAGC GAGTCGACAA TTGTCACCTC ATTCGACTCA TCTCACCTCA
     5041 CACGCCGCGC AGAATAAACA CGAGAGAGAT CAAAGTATGC TAGATTGAAG GGACTGCACA
     5101 TTCCATCGGA TTAGGGAAAA TATCGTGTTA CAAAATGTCG AGTAAGGGAG AGCTGACTTT
     5161 ATTGCCAATA ACTGCAAATT AGTCACATTT GACATTTTTT ACCATTTCGA GTTAATTGTG
     5221 CTTATTCTGC GCGGCGTGTG AGTCGAGACG AGTCGCTCTC ACCTCGGGTG ACGTGAGACG
     5281 ACTAATGTTA ATCAAATGGG AGCGAGTCGA CAATTGTCAC CTCATTCGAC TCGTGTCACC
     5341 TCACACGCCG CGCAGAATAG GCACGAATAC CATGCAGGGT CATCAAATGA TAAACACTTT
     5401 CGATCCACTT ACTGAGCTCT GTGAGATTGT TCTAGAAAAC TCGAAAAAAA AAATACCAAA
     5461 ATGTTTTGTC ACATTGGCAA TGGTAACCGC ATAACAGTCA CATTGAGATT ATATATGAGC
     5521 CTCGTAATGT AATGAAATAA TGAAATTTCT ATCAGATTTT TTTCTGGCAA TATCGCATAT
     5581 ATGAGCTGTA CGAAATTTCA GCATCAAATA AGCACTTTTG TGTTTTTGGT AATTTTAAGA
     5641 TATTTTAGTA TCTTCCCATA CTGCACACTC AGTTGAGCTC GGCTAATTTT CATTCTTTTG
     5701 CCGAGATCCG CACAGCTGAG CAACTGAATA AAGTGTGTGT TATGGGGCTT TGCACGAAAA
     5761 TCCATCCCTC TCTTTCACTA TGTGCTTTCA CTCTTCCATG AAATTAGAAA ACATCAAGAC
     5821 CAGTAAACGT CAAAATCCCA TACAAAATCA GAAACTTCAT AAAATGCACA CTTGGTACTC
     5881 CATTTACTCA ATGCCTACTT TTGTTGAATG CTTCAAATAT GCAAGGGATC GATCAGGTAT
     5941 CTGAGGAATT TTTTTCAAGC TCACGATCAA AAGCTACACA CGTGCTCATA TTTGTGTCCA
     6001 AGTTTTTTTT TCAAATACAG GCCTTATTCG AAGCTCAATT GCATAATACG CTCTAAGGAG
     6061 CCGACTTTTT AATTGTCCTA ATATAGTAGA ATGATGCCAA GGTACTAGAC ACAACTCAAT
     6121 TTTCTTAGGA TACGAATATG CTAGGATGGA TAGGACAATG ATGTGTTAAA AAGATTCACT
     6181 GGAGCTCTTC TGAGTACATC TTAGCAGCTT TAGGTAAGAG ACAATCCATA AGCCACGTGG
     6241 TCATTTTTTT GGGTTGTAGT CTTTTGTTCA AACAGAACAT TTAAAATTTG TGTGTCCACC
     6301 CTAAGTGAGC ACGTGGTTTA TGGACGACCG CGAAGGTAAC TTGTGTAGTT TCGGCAACTT
     6361 CTAGAGTCGA CCTGCAGGCA TGCAAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG
     6421 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
     6481 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
     6541 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
     6601 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
     6661 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
     6721 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
     6781 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
     6841 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
     6901 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
     6961 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
     7021 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
     7081 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
     7141 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
     7201 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
     7261 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
     7321 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
     7381 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     7441 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     7501 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     7561 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     7621 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     7681 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     7741 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     7801 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     7861 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     7921 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     7981 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     8041 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     8101 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     8161 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     8221 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     8281 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     8341 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     8401 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     8461 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     8521 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG
     8581 ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTC
//
