LOCUS       Exported                8817 bp    DNA     circular SYN 25-Feb-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 25, 2019 from SnapGene Viewer 4.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          <2289..>3740
                     /mol_type="other DNA"
                     /note=" [Split]"
                     /organism="synthetic DNA construct"
     source          <3741..>4978
                     /mol_type="other DNA"
                     /note=" [Split]"
                     /organism="synthetic DNA construct"
     source          join(<4979..>8817,<1..>1036)
                     /mol_type="other DNA"
                     /note=" [Split]"
                     /organism="synthetic DNA construct"
     source          join(6554..8817,1..416)
                     /label=Cloning vector pUC19
     misc_feature    1..59
                     /label=BsmBI
                     /note="BsmBI"
     gene            complement(146..416)
                     /label=lacZalpha
     CDS             complement(146..416)
                     /codon_start=1
                     /label=beta-galactosidase alpha fragment
                     /translation="PGTELEFTGRRFTTS*LGKPWRYPT*SPCSTSPFRQLA**RRGPH
                     RSPFPTVAQPEWRMAPDAVFSPYASVRYFTPHMVHSQYNLL*CRI"
     misc_feature    complement(146..>237)
                     /note="pBR322 [Split]"
     misc_feature    complement(238..395)
                     /note="Lac-operon"
     misc_feature    239..399
                     /note="lac operon"
     primer_bind     352..375
                     /note="pUC/M13 forward sequencing primer"
     primer_bind     379..394
                     /note="M13 forward"
     primer_bind     388..447
                     /label=Primer 21
                     /note="ATG_0234"
     misc_feature    388..416
                     /label=Gibson R homology
                     /note="Gibson R homology"
     misc_feature    388..416
                     /label=L Arm
                     /note="L Arm"
     primer_bind     417..447
                     /label=Set 2 - Pair 1
                     /note="ATG_0045"
     misc_feature    join(417..>2231,<4979..6553)
                     /label=Pair 1
                     /note=" [Split]"
     CDS             417..2231
     primer_bind     2027..2056
                     /label=Set 1 - Pair 1
                     /note="ATG_0047"
     misc_feature    2067..2085
                     /label=TGG_0028 ir25a Guide 3
                     /note="TGG_0028 ir25a Guide 3"
     CDS             2109..2231
                     /codon_start=1
                     /label=41 AA Before Stop
                     /note="41 AA Before Stop"
                     /translation="RLPNLKNEFGAMGQIKDDSQKTQSLRTRTQTIDANNFKSRF"
     primer_bind     complement(2110..2135)
                     /label=Primer 13
                     /note="ATG_0209"
     primer_bind     complement(2110..2169)
                     /label=Primer 14
                     /note="ATG_0210"
     misc_feature    2123..2142
                     /label=TGG_0026 ir25a Guide 1
                     /note="TGG_0026 ir25a Guide 1"
     misc_feature    2136..2158
                     /label=Modifed PAM and Guide
                     /note="Modifed PAM and Guide"
     misc_feature    2136..2154
                     /label=TGG_0029 ir25a Guide 4
                     /note="TGG_0029 ir25a Guide 4"
     primer_bind     complement(2145..2204)
                     /label=Primer 16
                     /note="ATG_0212"
     primer_bind     complement(2145..2169)
                     /label=Primer 15
                     /note="AtG_0211"
     misc_feature    2164..2183
                     /label=TGG_0030 ir25a Guide 5
                     /note="TGG_0030 ir25a Guide 5"
     misc_feature    2178..2200
                     /label=Modified Guide and PAM
                     /note="Modified Guide and PAM"
     misc_feature    2182..2200
                     /label=TGG_0027 ir25a Guide 2
                     /note="TGG_0027 ir25a Guide 2"
     primer_bind     complement(2185..>2231)
                     /label=Primer 18
                     /note="ATG_0214"
     primer_bind     complement(2185..2204)
                     /label=Primer 17
                     /note="ATG_0213"
     frag            2232..3740
                     /date="26-Mar-2019"
                     /note="1 to 1509 of T2A-QF2AD-SV40"
     misc_feature    2232..3740
     frag            <2232..>2288
                     /date="09-Feb-2018"
                     /note="1 to 4569 of IR7f_QF2_plasmid [Split]"
     variation       complement(<2232..2267)
                     /note=" [Split]"
     variation       complement(<2232..2244)
                     /note=" [Split]"
     variation       complement(<2232..2244)
                     /note="VJ_0225 [Split]"
     variation       <2232..2250
                     /note=" [Split]"
     misc_feature    2232..2288
                     /label=T2A
                     /note="T2A"
                     /note="; /ApEinfo_fwdcolor=""pink"
                     /note="; /ApEinfo_revcolor=""pink"
                     /note="; /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0
                     -1} {} 0} width 5 offset 0"""
     frag            2232..>2288
                     /date="08-Feb-2018"
                     /note="3218 to 5786 of pTS1_Dop1R1_del_ [Split]"
     variation       2232..2234
                     /note="T2A infame"
     frag            2232..2288
                     /date="25-Feb-2019"
                     /note="1417 to 1473 of IR7f_QF2_puc19"
     variation       complement(2257..2280)
                     /note="oTS101"
     frag            2289..3740
                     /date="25-Feb-2019"
                     /note="6769 to 8220 of Exported"
     misc_feature    2289..2345
                     /note="/ugene_name=""SV40NLS"
                     /note="; /ugene_group=""nls"""
     misc_feature    2352..2852
                     /note="/ugene_name=""QF2_AD"
                     /note="; /ugene_group=""gene"""
     misc_feature    2895..3035
                     /note="/mismatches=0"
     misc_feature    2895..3035
                     /note="/ugene_name=""zipEE12RR345L"
                     /note="; /ugene_group=""zip"""
     misc_feature    3047..>3740
                     /note=" [Split]"
     terminator      3055..3311
                     /note="hsp70 termination"
                     /note="color: #ffffff"
     CDS             3610..3740
     frag            3741..4978
                     /date="26-Mar-2019"
                     /left_end="0"
                     /note="1 to 1238 of 3xp3-eyfp from addgene"
                     /right_end="0"
     misc_feature    3741..4978
     misc_feature    3746..3795
                     /note="3x Pax6 binding sites"
     misc_feature    3835..3842
                     /note="TATA"
     gene            4006..4722
                     /note="EYFP (Clontech)"
     CDS             4006..4722
     misc_feature    4729..4971
                     /note="SV40 term sequences"
     primer_bind     4979..5011
                     /label=Primer 19
                     /note="ATG_0215"
     primer_bind     <4979..5011
                     /label=Primer 20
                     /note="ATG_0216"
     CDS             4979..6582
     primer_bind     5598..5610
                     /label=Primer 20
                     /note="ATG_0216"
     primer_bind     5598..5610
                     /label=Primer 19
                     /note="ATG_0215"
     primer_bind     complement(6527..6553)
                     /label=Set 2 - Pair 1 (2)
                     /note="ATG_0046"
     primer_bind     complement(6527..6582)
                     /label=Primer 22
                     /note="ATG_0235"
     misc_feature    6554..6582
                     /label=R Arm
                     /note="R Arm"
     primer_bind     6554..6571
                     /label=Primer 3
                     /note="ATG_0207"
     misc_feature    complement(6554..6582)
                     /label=Gibson F homology
                     /note="Gibson F homology"
     primer_bind     6554..6571
                     /label=Primer 2
                     /note="ATG_0207"
     primer_bind     6554..6571
                     /label=Primer 4
                     /note="ATG_0207"
     primer_bind     6554..6571
                     /label=Primer 1
                     /note="ATG_0207"
     misc_feature    complement(6586..6815)
                     /note="Lac-operon"
     misc_feature    6586..6833
                     /note="lac operon sequence"
     primer_bind     6596..6612
                     /note="binding site of pUC/M13 reverse sequencing primer"
     primer_bind     complement(6596..6617)
                     /note="pUC/M13 reverse sequencing primer"
     promoter        complement(6601..6722)
                     /note="Plac"
     primer_bind     complement(6609..6632)
                     /note="Ptac forward sequencing primer (NEB #1260,
                     discontinued)"
     protein_bind    complement(6616..6640)
                     /bound_moiety="LacI"
                     /note="lac operator"
     -10_signal      complement(6645..6650)
                     /label=Plac promoter
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(6669..6674)
                     /label=Plac promoter
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     protein_bind    complement(6694..6706)
                     /label=CAP protein binding site
                     /note="CAP protein binding site"
     misc_feature    complement(6815..6878)
                     /citation=[5]
                     /note="L-strand Y effector site"
     misc_feature    complement(join(6816..8817,1..237))
                     /note="pBR322"
     rep_origin      6938..7822
                     /note="pUC Origin"
     rep_origin      complement(6939..7612)
                     /direction=LEFT
                     /label=pMB1 origin of replication
                     /note="pMB1 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     rep_origin      6995..7031
                     /note="ColE1-derived origin of replication"
     rep_origin      6998..7586
                     /note="pMB1 origin of replication (counterclockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(6998..7550)
                     /label=RNAII transcript
                     /note="RNAII transcript (complementary strand)"
     -35_signal      7404..7409
                     /label=RNAI promoter
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      7426..7431
                     /label=RNAI promoter
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        7440..7547
                     /label=RNAI transcript
                     /note="RNAI transcript"
     -10_signal      complement(7560..7565)
                     /label=RNAII promoter
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(7581..7586)
                     /label=RNAII promoter
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     repeat_region   join(7612..8817,1)
                     /note="transposon=Tn3 [Split]"
     mobile_element  join(7612..8817,1)
                     /mobile_element_type="transposon:Tn3"
     gene            complement(7757..8617)
                     /label=bla
     CDS             complement(7757..8617)
                     /codon_start=1
                     /label=beta-lactamase
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(7760..8611)
                     /db_xref="CDD:185339"
                     /note="beta-lactamase TEM; Provisional; Region: PRK15442"
     mat_peptide     complement(7760..8548)
                     /gene="bla"
                     /note=" [Split]"
                     /product="beta-lactamase"
     misc_feature    7897..7902
                     /label=BsaI
                     /note="BsaI"
     sig_peptide     complement(8549..8617)
                     /label=bla
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     promoter        complement(8659..8687)
                     /label=pAmp
                     /note="AmpR_promoter"
     -10_signal      complement(8661..8666)
                     /label=bla promoter
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(8682..8687)
                     /label=bla promoter
                     /note="bla promoter (counter-clockwise); TTCAAA"
BASE COUNT      2456 A      2028 C      2063 G      2270 T         0 OTHER
ORIGIN      ?
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGATCG
      421 AGTTTATACT GTGGTGGTAA GTTATTAATA TTTTTAAGGA TATTCCAGGC CTTTCAAAAA
      481 AACATTGATT GATTACCTTT ACCTACAATC TTTACATTGT TTCGTAGCAA GAACCATTCA
      541 TTATACGAGA TCCAACTGCT CCCAAAGGTT TCAAAGGTTA TTGTATCGAC TTGTTGGATG
      601 AAATTGCAAA AATTGTTAAG TTCGATTATG AAATCAAGGA AGTTGAAGAT GGCAAGTTTG
      661 GAAACATGAA CGAAAAAGGT GAATGGAACG GTATCGTACG CAAATTGATC GACAAGCAAG
      721 CCGATATCGG TTTAGGATCG ATGTCCGTGA TGGCCGAGCG GGAAACTGTA ATTGATTTCA
      781 CGGTGCCATA TTACGATTTG GTTGGAATAT CAATCATGAT GCTGCTGCCC AGTACACCGA
      841 GTTCGCTGTT CAAATTTTTG ACCGTGTTGG AGACGAATGT GTGGCTTTGT ATTCTCGCAG
      901 CGTATTTCTT CACTAGTTTT CTCATGTGGA TTTTTGATCG GTACAGTCCT TACAGTTACC
      961 AGAACAATCG TGAAAAGTAC AAGAATGATG ATGAAAAACG AGAGTTCAAC ATTAAGGAAT
     1021 GCCTATGGTT TTGTATGACT TCGTTAACGC CGCAAGGAGG TGGTGAAGCT CCGAAAAATT
     1081 TATCCGGACG GCTGGTGGCG GCAACGTGGT GGTTGTTTGG GTAAGTATTC TACTGAATTT
     1141 TGTTTAGATA ATAAATATTT AATGGATGAT TTATGTAAAA CATAAAGATT CATCATCATT
     1201 GCATCGTACA CTGCTAACTT GGCTGCTTTC CTGACCGTAT CGCGATTGGA CACTCCCGTG
     1261 GAATCGTTGG ACGATCTTTC GAAGCAGTAT AAAATTTTGT ACGCCCCTTT GAACGGATCT
     1321 TCGGCTATGA CTTATTTCCA GCGAATGGCG GATATCGAAG CCCGTTTCTA TGAGTAAGTT
     1381 TGTTCTCATT CATGCTTACT AGGGGTAATT TTCTAAGCAA AATCACTCTC CACAGAATTT
     1441 GGAAAGAGAT GTCGCTGAAT GATTCTCTCA CACCCGTGGA GCGCTCGAAG CTGGCGGTTT
     1501 GGGACTATCC CGTCAGTGAT AAGTATACGA AAATGTGGCA AGCAATGCAG GAAGCTGGAC
     1561 TCCCAAATAG TTTGGATGAA GCGGTCGAGC GTATTAGGAA CTCCACATCG GCCTCTGGTT
     1621 TCGCTTTTCT GGGCGATGCT ACTGATATAC GATACAAAGT ACTTACAAAC TGTGATCTAC
     1681 AGATGGTCGG CGAAGAATTT TCTCGCAAAC CGTATGCCAT TGCAGTACAG CAAGGATCGC
     1741 CGTTGAAAGA TCAATTCAAC AATGCGTAAG TTTAGCTGAC GGATGTCCTA TTCAATATAA
     1801 CAACTTAATA TACCATTATA CCTTCTAGCA TCTTGATGCT TCTCAACAAA CGACAACTTG
     1861 AAAAACTCAA GGAACAATGG TGGAAAAATG ATGACATTCA AAGCAAATGT GAAAAACCGG
     1921 ACGACCAATC CGATGGTATT TCGATTCAGA ACATTGGAGG TGTCTTCATA GTGATCTTCG
     1981 TCGGCATTGG AATGGCGTGC ATTACGCTGG TCTTTGAATT CTGGTACTAC AAATACCGCA
     2041 AAAACGTAAA GATTATTGAT GTCGCCGAGG CTACGGAAGA TAAACTGGCT CAGAAGACGT
     2101 CCAATTTGAG GTTGCCAAAT TTGAAGAACG AATTTGGAGC CATGGGACAG ATCAAAGATG
     2161 ACAGTCAGAA AACGCAAAGT TTGCGAACTC GTACGCAGAC GATAGACGCC AACAACTTTA
     2221 AATCTCGTTT TGGAGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC
     2281 CCGGCCCTAT GGATAAAGCG GAATTAATTC CCGAGCCTCC AAAAAAGAAG AGAAAGGTCG
     2341 AATTGGACGT CCGTCAGTTG GAGCTCCCTC CTACCGCCAC GGCTACGGCC TCGATAATGC
     2401 CGCACGTGAT GGAGCAGCCT CTCAGTACCA GCATTAACCC CGTCAACGAC CGCTTCAACG
     2461 GTATTCCCAA CCCCACTCCG TATAACTCCG ATGCAGCTCT CGATGCTATC ACTCAGACCA
     2521 ACGATTATGG AAGCGTAAAT ACACATGGTA TCCTCTCTAC TTACCCGCCA CCGGCTACGC
     2581 ACCTTAATGA AGCTTCCGTC GCTCTCGCTC CCGGTGGCGC CCCCCCCCGA CCGCCTCCTC
     2641 CGTATGTTGA CAGCACGACC AATCACCCGC CGTACCACTC GAATCTGGTT CCAATGGCGA
     2701 ACTTTGGTTA CTCGACCGTT GATTACGATG CCATGGTTGA CGATTTGGCT AGCATTGAAT
     2761 ACACGGACGC TGTGGATGTC GACCCACAGT TTATGACCAA TCTGGGATTC GTTCCTGGAT
     2821 GTAACTTCTC CGACATTAAT ACATACGAAC AGAGATCTGG AGGAGGTGGT GGAGGTGGAG
     2881 GTGGAGGTAC TAGTCTGGAG ATCGAGGCCG CCTTCCTGGA GCGCGAGAAC ACCGCCCTGG
     2941 AGACCCGCGT GGCCGAGCTG CGCCAGCGCG TGCAGCGCCT GCGCAACCGC GTGAGCCAGT
     3001 ACCGCACCCG CTACGGCCCC CTGGGCGGCG GCAAGTAAGG CCGGCCGATC TTTGTGAAGG
     3061 AACCTTACTT CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA
     3121 AGGTAAATAT AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG
     3181 TATTTTAGAT TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG
     3241 AGGAAAACCT GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT
     3301 CTCAACATTC TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT
     3361 CAGAATTGCT AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG
     3421 CTATTTACAC CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT
     3481 TGATGTATAG TGCCTTGACT AGAGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA
     3541 CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT
     3601 GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA
     3661 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC
     3721 AATGTATCTT ATCATGTCTG CGCCCGGGGA TCTAATTCAA TTAGAGACTA ATTCAATTAG
     3781 AGCTAATTCA ATTAGGATCC AAGCTTATCG ATTTCGAACC CTCGACCGCC GGAGTATAAA
     3841 TAGAGGCGCT TCGTCTACGG AGCGACAATT CAATTCAAAC AAGCAAAGTG AACACGTCGC
     3901 TAAGCGAAAG CTAAGCAAAT AAACAAGCGC AGCTGAACAA GCTAAACAAT CGGGGTACCG
     3961 CTAGAGTCGA CGGTACCGCG GGCCCGGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC
     4021 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
     4081 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
     4141 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCTTC
     4201 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
     4261 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
     4321 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
     4381 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
     4441 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
     4501 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
     4561 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
     4621 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
     4681 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGCGACTCT
     4741 AGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC
     4801 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG
     4861 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
     4921 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA AGCTTATCGA
     4981 TACTGAAGTT CATTTTTTGA GTAGTAACGT GTTGTTTGAA ATTACCCAAT AGAGAAGAAA
     5041 CATATTTTTG TCTATCGCAT ATTAACAGGT TCCACTTGAA TTTTTAGATT TCTTCAATTA
     5101 TTTTTTTTTG TTTCATAATC AGAAGAAATA TGAAAATTGG ACTGGATCAG TTCTTCTTTC
     5161 TCGACTCGTA TTTTTTTCTT CTCGAAAAAA TTGGAACACT ATGGAAAAAC GCTTAAGATC
     5221 TCTATTCTAC CCTAGTTTAA CTAGTACAAT AAACATCTTG GCTACATTAA ATTTATTCTG
     5281 TGGATAAATC TGAACACACA TTTTCTACAT CGATTTATAC TAGGATTCAG ACCCACAGAG
     5341 TAATAGAAAT ACAACCCTGT GGAATTCGCA TTGTATCGTA AAGCTAGTGG GCTACAATCA
     5401 AAGTGTAGTC GTAAAAGGTC TTTTTCGAAA ATTTTTGAAT TGTATAAAAG TTCTCGCAAA
     5461 AGATCTAATA TCTAGTATCC CACACAGGAT GCGATGCATG GAATTCCAAC AACAGCAAAA
     5521 AAAAACGCGT ATCGAATTTA AGATACGAGG GGTAGTAACG TGTTGTTATA TCATTCTTGA
     5581 TATAATCGTT ATTTTGAGAG TAGTAACGTG TTAGTAGGAT TGGTTATGTA ATTTTAATAC
     5641 AGTAGCTAGT AAGATCATCC CAAATCATTT AAGGGTGTAT TATGGAAATT GAGTCGACCT
     5701 CATGAGAAAA AAGTCATCTC AATTTACATG ATTGTTTCCT TACAATGTTT GCATCCAATC
     5761 CGAAATTCCT TAACAACTTT TCATACATTC TCGAGCACTT TATGCTTAAA AGCCCCGAAG
     5821 GCTTTCCGGT CTGTCTCGTT CAACGTCCAC GTTTCATGTC GGCAAGAAGC CACCAGATGT
     5881 ATCCGCTTTT CACGCATTTT GTTTTTATTT TGGGGTTGTG GATTTTAAGC TGGTTACAAA
     5941 GTTCTCCCAC CACTACCAAG CGGTTGCCTG GTTCTCTAGA TTTGTGCTCT TGAAAATTCG
     6001 AGGTTTGGCT TCGATGCATC ATCACCTTAA TCTTCATATG CGAGATAAAC TGGTTGAACG
     6061 ATCCCCCAAA ATCGTAGAGA TCCTAGACTA TAGAGAACAA TCCAATAATG TATGTTAAAG
     6121 CCGACGTCGG ATAGCGCTTC CAAAAGAACC ACGAGTGGGA AAAAAGAAAG TGAAGAAAAT
     6181 TTGAAACTGG CGGTCAAGAA ATATCCGAAA CCGAAGACAG TGTAACAAAA GAACTTAGGC
     6241 CGTCGTTATT AATTGGAGGA AGCGGAATAC GCCAAAACCC TGAAGTCGAT GCGAAGCACT
     6301 GAGAAGCTAT CCGCCTAATG ACGACATCGT GCAAACTAGC ATCGATGAAA TGAAGTTAAA
     6361 AAAACAAACA AACTAGTGTA GTCTGTTGTG TGGAAGAGCC GGAAGGTGGC TTGGACGAGG
     6421 TGCTCAAGAA TTGGAGGCCA AGATGCTGAG AACGGGGCTG AAGGTGCGGA TGTCGGTTGA
     6481 TCGGACGACG AGCTCGGAAG TGCTGGAGGA ACTCGGAAGT CAAGAGGGGC TTTTCTATAG
     6541 TAGGGTACAA AAATCTAGAG TCGACCTGCA GGCATGCAAG CTTGGCGTAA TCATGGTCAT
     6601 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
     6661 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC
     6721 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC
     6781 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT
     6841 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
     6901 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
     6961 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
     7021 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
     7081 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
     7141 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
     7201 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
     7261 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
     7321 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
     7381 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA
     7441 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
     7501 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
     7561 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
     7621 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
     7681 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
     7741 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
     7801 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
     7861 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
     7921 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
     7981 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
     8041 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
     8101 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
     8161 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
     8221 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
     8281 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
     8341 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
     8401 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
     8461 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
     8521 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
     8581 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
     8641 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
     8701 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA
     8761 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTC
//
