LOCUS       Exported                6401 bp ds-DNA     circular SYN 27-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Amaia
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 22, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    458..1718
                     /label=EF1A
     misc_feature    1772..2869
                     /label=CCNE1
     polyA_signal    3030..3151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3780..3796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3804..3820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3828..3858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3873..3894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4182..4770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4941..5801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5802..5906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6380..6396
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcgagct cggtacccta cagttgaagt cggaagttta catacactta agttggagtc
       61 attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat agttttggca
      121 agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca attgtttaca
      181 gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag aagtttacat
      241 acactaagtt gactgtgcct ttaaacagct tggaaaattc cagaaaatga tgtcatggct
      301 ttagaagctt ctgatagact aattgacatc atttgagtca attggaggtg tacctgtgga
      361 tgtatttcaa gggaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
      421 ggctccccag gcaggcagaa gtatgcaaag catgcattta attaaaatta tctctaaggc
      481 atgtgaactg gctgtcttgg ttttcatctg tacttcatct gctacctctg tgacctgaaa
      541 catatttata attccattaa gctgtgcata tgatagattt atcatatgta ttttccttaa
      601 aggatttttg taagaactaa ttgaattgat acctgtaaag tctttatcac actacccaat
      661 aaataataaa tctctttgtt cagctctctg tttctataaa tatgtaccag ttttattgtt
      721 tttagtggta gtgattttat tctctttcta tatatataca cacacatgtg tgcattcata
      781 aatatataca atttttatga ataaaaaatt attagcaatc aatattgaaa accactgatt
      841 tttgtttatg tgagcaaaca gcagattaaa aggaatttca attgcctgca ggagtcaatg
      901 ggaaaaaccc attggagcca agtacactga ctcaataggg actttccatt gggttttgcc
      961 cagtacataa ggtcaatagg gggtgagtca acaggaaagt cccattggag ccaagtacat
     1021 tgagtcaata gggactttcc aatgggtttt gcccagtaca taaggtcaat gggaggtaag
     1081 ccaatgggtt tttcccatta ctgacatgta tactgagtca ttagggactt tccaatgggt
     1141 tttgcccagt acataaggtc aataggggtg aatcaacagg aaagtcccat tggagccaag
     1201 tacactgagt caatagggac tttccattgg gttttgccca gtacaaaagg tcaatagggg
     1261 gtgagtcaat gggtttttcc cattattggc acatacataa ggtcaatagg ggtgactagt
     1321 ggagaagagc atgcttgagg gctgagtgcc cctcagtggg cagagagcac atggcccaca
     1381 gtccctgaga agttgggggg aggggtgggc aattgaactg gtgcctagag aaggtggggc
     1441 ttgggtaaac tgggaaagtg atgtggtgta ctggctccac ctttttcccc agggtggggg
     1501 agaaccatat ataagtgcag tagtctctgt gaacattcaa gcttctgcct tctccctcct
     1561 gtgagtttgg taagtcactg actgtctatg cctgggaaag ggtgggcagg agatggggca
     1621 gtgcaggaaa agtggcacta tgaaccctgc agccctagac aattgtacta accttcttct
     1681 ctttcctctc ctgacaggtt ggtgtacagt agcttccaag tactaagatc tagtgcacag
     1741 ggcccaccat ggagctagct ggggggatct catgccgagg gagcgcaggg agcgggatgc
     1801 gaaggagcgg gacaccatga aggaggacgg cggcgcggag ttctcggctc gctccaggaa
     1861 gaggaaggca aacgtgaccg tttttttgca ggatccagat gaagaaatgg ccaaaatcga
     1921 caggacggcg agggaccagt gtgggagcca gccttgggac aataatgcag tctgtgcaga
     1981 cccctgctcc ctgatcccca cacctgacaa agaagatgat gaccgggttt acccaaactc
     2041 aacgtgcaag cctcggatta ttgcaccatc cagaggctcc ccgctgcctg tactgagctg
     2101 ggcaaataga gaggaagtct ggaaaatcat gttaaacaag gaaaagacat acttaaggga
     2161 tcagcacttt cttgagcaac accctcttct gcagccaaaa atgcgagcaa ttcttctgga
     2221 ttggttaatg gaggtgtgtg aagtctataa acttcacagg gagacctttt acttggcaca
     2281 agatttcttt gaccggtata tggcgacaca agaaaatgtt gtaaaaactc ttttacagct
     2341 tattgggatt tcatctttat ttattgcagc caaacttgag gaaatctatc ctccaaagtt
     2401 gcaccagttt gcgtatgtga cagatggagc ttgttcagga gatgaaattc tcaccatgga
     2461 attaatgatt atgaaactgt tggatctctg tgtcctggat gttgactgcc ttgaatttcc
     2521 ttatggtata cttgctgctt cggccttgta tcatttctcg tcatctgaat tgatgcaaaa
     2581 ggtttcaggg tatcagtggt gcgacataga gaactgtgtc aagtggatgg ttccatttgc
     2641 catggttata agggagacgg ggagctcaaa actgaagcac ttcaagggcg tcgctgatga
     2701 agatgcacac aacatacaga cccacagaga cagcttggat ttgctggaca aagcccgagc
     2761 aaagaaagcc atgttgtctg aacaaaatag ggcttctcct ctccccagtg ggctcctcac
     2821 cccgccacag agcggtaaga agcagagcag cgggccggaa atggcgtgaa tcagtggtcc
     2881 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
     2941 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     3001 ggtttggcaa gctagctggc cagacatgat aagatacatt gatgagtttg gacaaaccac
     3061 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3121 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3181 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     3241 tatggaattg gagccccact gtgttcatct tacagatgga aatactgaca ttcagaggag
     3301 ttagttaact tgcctagcgg ccgctgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3361 tatcttatca tgtctggatc cccttgaaat acatccacag gtacacctcc aattgactca
     3421 aatgatgtca attagtctat cagaagcttc taaagccatg acatcatttt ctggaatttt
     3481 ccaagctgtt taaaggcaca gtcaacttag tgtatgtaaa cttctgaccc actggaattg
     3541 tgatacagtg aattataagt gaaataatct gtctgtaaac aattgttgga aaaatgactt
     3601 gtgtcatgca caaagtagat gtcctaactg acttgccaaa actattgttt gttaacaaga
     3661 aatttgtgga gtagttgaaa aacgagtttt aatgactcca acttaagtgt atgtaaactt
     3721 ccgacttcaa ctgtatatct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg
     3781 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3841 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3901 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3961 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4021 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4081 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4141 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4201 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4261 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4321 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     4381 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4441 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4501 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4561 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4621 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4681 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4741 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4801 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4861 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4921 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4981 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5041 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5101 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5161 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5221 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5281 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5341 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5401 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5461 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5521 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5581 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5641 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5701 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5761 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5821 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5881 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     5941 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     6001 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     6061 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     6181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     6241 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     6301 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6361 ttttcccagt cacgacgttg taaaacgacg gccagtgaat t
//
