LOCUS       Exported                6518 bp ds-DNA     circular SYN 27-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Amaia
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 22, 2020 from SnapGene Viewer 5.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    458..1717
                     /label=EF1A
     misc_feature    1772..2985
                     /label=CCNE2
     polyA_signal    3147..3268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3897..3913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3921..3937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3945..3975)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3990..4011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4299..4887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5058..5918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5919..6023)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6497..6513
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcgagct cggtacccta cagttgaagt cggaagttta catacactta agttggagtc
       61 attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat agttttggca
      121 agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca attgtttaca
      181 gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag aagtttacat
      241 acactaagtt gactgtgcct ttaaacagct tggaaaattc cagaaaatga tgtcatggct
      301 ttagaagctt ctgatagact aattgacatc atttgagtca attggaggtg tacctgtgga
      361 tgtatttcaa gggaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
      421 ggctccccag gcaggcagaa gtatgcaaag catgcattta attaaaatta tctctaaggc
      481 atgtgaactg gctgtcttgg ttttcatctg tacttcatct gctacctctg tgacctgaaa
      541 catatttata attccattaa gctgtgcata tgatagattt atcatatgta ttttccttaa
      601 aggatttttg taagaactaa ttgaattgat acctgtaaag tctttatcac actacccaat
      661 aaataataaa tctctttgtt cagctctctg tttctataaa tatgtaccag ttttattgtt
      721 tttagtggta gtgattttat tctctttcta tatatataca cacacatgtg tgcattcata
      781 aatatataca atttttatga ataaaaaatt attagcaatc aatattgaaa accactgatt
      841 tttgtttatg tgagcaaaca gcagattaaa aggaatttca attgcctgca ggagtcaatg
      901 ggaaaaaccc attggagcca agtacactga ctcaataggg actttccatt gggttttgcc
      961 cagtacataa ggtcaatagg gggtgagtca acaggaaagt cccattggag ccaagtacat
     1021 tgagtcaata gggactttcc aatgggtttt gcccagtaca taaggtcaat gggaggtaag
     1081 ccaatgggtt tttcccatta ctgacatgta tactgagtca ttagggactt tccaatgggt
     1141 tttgcccagt acataaggtc aataggggtg aatcaacagg aaagtcccat tggagccaag
     1201 tacactgagt caatagggac tttccattgg gttttgccca gtacaaaagg tcaatagggg
     1261 gtgagtcaat gggtttttcc cattattggc acatacataa ggtcaatagg ggtgactagt
     1321 ggagaagagc atgcttgagg gctgagtgcc cctcagtggg cagagagcac atggcccaca
     1381 gtccctgaga agttgggggg aggggtgggc aattgaactg gtgcctagag aaggtggggc
     1441 ttgggtaaac tgggaaagtg atgtggtgta ctggctccac ctttttcccc agggtggggg
     1501 agaaccatat ataagtgcag tagtctctgt gaacattcaa gcttctgcct tctccctcct
     1561 gtgagtttgg taagtcactg actgtctatg cctgggaaag ggtgggcagg agatggggca
     1621 gtgcaggaaa agtggcacta tgaaccctgc agccctagac aattgtacta accttcttct
     1681 ctttcctctc ctgacaggtt ggtgtacagt agcttccaag tactaagatc tagtgcacag
     1741 ggcccaccat ggagctagct ggggggatct catgtcaaga cgaagtagcc gtttacaagc
     1801 taagcagcag ccccagccca gccagacgga atccccccaa gaagcccaga taatccaggc
     1861 caagaagagg aaaactaccc aggatgtcaa aaaaagaaga gaggaggtca ccaagaaaca
     1921 tcagtatgaa attaggaatt gttggccacc tgtattatct ggggggatca gtccttgcat
     1981 tatcattgaa acacctcaca aagaaatagg aacaagtgat ttctccagat ttacaaatta
     2041 cagatttaaa aatcttttta ttaatccttc acctttgcct gatttaagct ggggatgttc
     2101 aaaagaagtc tggctaaaca tgttaaaaaa ggagagcaga tatgttcatg acaaacattt
     2161 tgaagttctg cattctgact tggaaccaca gatgaggtcc atacttctag actggctttt
     2221 agaggtatgt gaagtataca cacttcatag ggaaacattt tatcttgcac aagacttttt
     2281 tgatagattt atgttgacac aaaaggatat aaataaaaat atgcttcaac tcattggaat
     2341 tacctcatta ttcattgctt ccaaacttga ggaaatctat gctcctaaac tccaagagtt
     2401 tgcttacgtc actgatggtg cttgcagtga agaggatatc ttaaggatgg aactcattat
     2461 attaaaggct ttaaaatggg aactttgtcc tgtaacaatc atctcctggc taaatctctt
     2521 tctccaagtt gatgctctta aagatgctcc taaagttctt ctacctcagt attctcagga
     2581 aacattcatt caaatagctc agcttttaga tctgtgtatt ctagccattg attcattaga
     2641 gttccagtac agaatactga ctgctgctgc cttgtgccat tttacctcca ttgaagtggt
     2701 taagaaagcc tcaggtttgg agtgggacag tatttcagaa tgtgtagatt ggatggtacc
     2761 ttttgtcaat gtagtaaaaa gtactagtcc agtgaagctg aagactttta agaagattcc
     2821 tatggaagac agacataata tccagacaca tacaaactat ttggctatgc tggaggaagt
     2881 aaattacata aacaccttca gaaaaggggg acagttgtca ccagtgtgca atggaggcat
     2941 tatgacacca ccgaagagca ctgaaaaacc accaggaaaa cactaaatca gtggtccagg
     3001 ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa
     3061 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     3121 ttggcaagct agctggccag acatgataag atacattgat gagtttggac aaaccacaac
     3181 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3241 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3301 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     3361 ggaattggag ccccactgtg ttcatcttac agatggaaat actgacattc agaggagtta
     3421 gttaacttgc ctagcggccg ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3481 cttatcatgt ctggatcccc ttgaaataca tccacaggta cacctccaat tgactcaaat
     3541 gatgtcaatt agtctatcag aagcttctaa agccatgaca tcattttctg gaattttcca
     3601 agctgtttaa aggcacagtc aacttagtgt atgtaaactt ctgacccact ggaattgtga
     3661 tacagtgaat tataagtgaa ataatctgtc tgtaaacaat tgttggaaaa atgacttgtg
     3721 tcatgcacaa agtagatgtc ctaactgact tgccaaaact attgtttgtt aacaagaaat
     3781 ttgtggagta gttgaaaaac gagttttaat gactccaact taagtgtatg taaacttccg
     3841 acttcaactg tatatctaga gtcgacctgc aggcatgcaa gcttggcgta atcatggtca
     3901 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3961 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4021 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4081 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4141 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4261 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     4501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     4741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5161 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5281 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5341 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5401 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5461 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5521 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5581 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5641 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5701 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5761 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5821 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5881 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5941 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6001 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     6061 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     6121 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     6181 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     6241 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6301 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     6361 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6421 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6481 tcccagtcac gacgttgtaa aacgacggcc agtgaatt
//
