LOCUS Exported 7967 bp ds-DNA circular SYN 06-MAR-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7967) AUTHORS Amaia TITLE Direct Submission JOURNAL Exported Thursday, Oct 22, 2020 from SnapGene Viewer 5.2.0 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7967 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 458..1718 /label=EF1A misc_feature 1772..4435 /label=mTert polyA_signal 4596..4717 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5346..5362) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 5370..5386 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5394..5424) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5439..5460 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5748..6336) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6507..7367) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7368..7472) /gene="bla" /label=AmpR promoter primer_bind 7946..7962 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 aattcgagct cggtacccta cagttgaagt cggaagttta catacactta agttggagtc 61 attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat agttttggca 121 agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca attgtttaca 181 gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag aagtttacat 241 acactaagtt gactgtgcct ttaaacagct tggaaaattc cagaaaatga tgtcatggct 301 ttagaagctt ctgatagact aattgacatc atttgagtca attggaggtg tacctgtgga 361 tgtatttcaa gggaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 421 ggctccccag gcaggcagaa gtatgcaaag catgcattta attaaaatta tctctaaggc 481 atgtgaactg gctgtcttgg ttttcatctg tacttcatct gctacctctg tgacctgaaa 541 catatttata attccattaa gctgtgcata tgatagattt atcatatgta ttttccttaa 601 aggatttttg taagaactaa ttgaattgat acctgtaaag tctttatcac actacccaat 661 aaataataaa tctctttgtt cagctctctg tttctataaa tatgtaccag ttttattgtt 721 tttagtggta gtgattttat tctctttcta tatatataca cacacatgtg tgcattcata 781 aatatataca atttttatga ataaaaaatt attagcaatc aatattgaaa accactgatt 841 tttgtttatg tgagcaaaca gcagattaaa aggaatttca attgcctgca ggagtcaatg 901 ggaaaaaccc attggagcca agtacactga ctcaataggg actttccatt gggttttgcc 961 cagtacataa ggtcaatagg gggtgagtca acaggaaagt cccattggag ccaagtacat 1021 tgagtcaata gggactttcc aatgggtttt gcccagtaca taaggtcaat gggaggtaag 1081 ccaatgggtt tttcccatta ctgacatgta tactgagtca ttagggactt tccaatgggt 1141 tttgcccagt acataaggtc aataggggtg aatcaacagg aaagtcccat tggagccaag 1201 tacactgagt caatagggac tttccattgg gttttgccca gtacaaaagg tcaatagggg 1261 gtgagtcaat gggtttttcc cattattggc acatacataa ggtcaatagg ggtgactagt 1321 ggagaagagc atgcttgagg gctgagtgcc cctcagtggg cagagagcac atggcccaca 1381 gtccctgaga agttgggggg aggggtgggc aattgaactg gtgcctagag aaggtggggc 1441 ttgggtaaac tgggaaagtg atgtggtgta ctggctccac ctttttcccc agggtggggg 1501 agaaccatat ataagtgcag tagtctctgt gaacattcaa gcttctgcct tctccctcct 1561 gtgagtttgg taagtcactg actgtctatg cctgggaaag ggtgggcagg agatggggca 1621 gtgcaggaaa agtggcacta tgaaccctgc agccctagac aattgtacta accttcttct 1681 ctttcctctc ctgacaggtt ggtgtacagt agcttccaag tactaagatc tagtgcacag 1741 ggcccaccat ggagctagct ggggggatct catgacccgc gctcctcgtt gccccgcggt 1801 gcgctctctg ctgcgcagcc gataccggga ggtgtggccg ctggcaacct ttgtgcggcg 1861 cctggggccc gagggcaggc ggcttgtgca acccggggac ccgaagatct accgcacttt 1921 ggttgcccaa tgcctagtgt gcatgcactg gggctcacag cctccacctg ccgacctttc 1981 cttccaccag gtgtcatccc tgaaagagct ggtggccagg gttgtgcaga gactctgcga 2041 gcgcaacgag agaaacgtgc tggcttttgg ctttgagctg cttaacgagg ccagaggcgg 2101 gcctcccatg gccttcacta gtagcgtgcg tagctacttg cccaacactg ttattgagac 2161 cctgcgtgtc agtggtgcat ggatgctact gttgagccga gtgggcgacg acctgctggt 2221 ctacctgctg gcacactgtg ctctttatct tctggtgccc cccagctgtg cctaccaggg 2281 gagatggcca agagcgtcta aacccctcat tcctactcag caacctccag cctaacttga 2341 ctggggccag gagactggtg gagatcatct ttctgggctc aaggcctagg acatcaggac 2401 cactctgcag gacacaccgt ctatcgcgtc gatactggca gatgcggccc ctgttccaac 2461 agctgctggt gaaccatgca gagtgccaat atgtcagact cctcaggtca cattgcaggt 2521 ttcgaacagc aaaccaacag gtgacagatg ccttgaacac cagcccaccg cacctcatgg 2581 atttgctccg cctgcacagc agtccctggc agggaaggac cgtgtccccg ctgcagagca 2641 ccgtctgagg gagaggatcc tggctacgtt cctgttctgg ctgatggaca catacgtggt 2701 acagctgctt aggtcattct tttacatcac agagagcaca ttccagaaga acaggctctt 2761 cttctaccgt aagagtgtgt ggagcaagct gcagagcatt ggagtcaggc aacaccttga 2821 gagagtgcgg ctacgggagc tgtcacaaga ggaggtcagg catcaccagg acacctggct 2881 agccatgccc atctgcagac tgcgcttcat ccccaagccc aacggcctgc ggcccattgt 2941 gaacatgagt tatagcatgg gtaccagagc tttgggcaga aggaagcagg cccagcattt 3001 cacccagcgt ctcaagactc tcttcagcat gctcaactat gagcggacaa aacatcctca 3061 ccttatgggg tcttctgtac tgggtatgaa tgacatctac aggacctggc gggcctttgt 3121 gctgcgtgtg cgtgctctgg accagacacc caggatgtac tttgttaagg cagatgtgac 3181 cggggcctat gatgccatcc cccagggtaa gctggtggag gttgttgcca atatgatcag 3241 gcactcggag agcacgtact gtatccgcca gtatgcagtg gtccggagag atagccaagg 3301 ccaagtccac aagtccttta ggagacaggt caccaccctc tctgacctcc agccatacat 3361 gggccagttc cttaagcatc tgcaggattc agatgccagt gcactgagga actccgttgt 3421 catcgagcag agcatctcta tgaatgagag cagcagcagc ctgtttgact tcttcctgca 3481 cttcctgcgt cacagtgtcg taaagattgg tgacaggtgc tatacgcagt gccagggcat 3541 cccccagggc tccagcctat ccaccctgct ctgcagtctg tgtttcggag acatggagaa 3601 caagctgttt gctgaggtgc agcgggatgg gttgctttta cgttttgttg atgactttct 3661 gttggtgacg cctcacttgg accaagcaaa aaccttcctc agcaccctgg tccatggcgt 3721 tcctgagtat gggtgcatga taaacttgca gaagacagtg gtgaacttcc ctgtggagcc 3781 tggtaccctg ggtggtgcag ctccatacca gctgcctgct cactgcctgt ttccctggtg 3841 tggcttgctg ctggacactc agactttgga ggtgttctgt gactactcag gttatgccca 3901 gacctcaatt aagacgagcc tcaccttcca gagtgtcttc aaagctggga agaccatgcg 3961 gaacaagctc ctgtcggtct tgcggttgaa gtgtcacggt ctatttctag acttgcaggt 4021 gaacagcctc cagacagtct gcatcaatat atacaagatc ttcctgcttc aggcctacag 4081 gttccatgca tgtgtgattc agcttccctt tgaccagcgt gttaggaaga acctcacatt 4141 ctttctgggc atcatctcca gccaagcatc ctgctgctat gctatcctga aggtcaagaa 4201 tccaggaatg acactaaagg cctctggctc ctttcctcct gaagccgcac attggctctg 4261 ctaccaggcc ttcctgctca agctggctgc tcattctgtc atctacaaat gtctcctggg 4321 acctctgagg acagcccaaa aactgctgtg ccggaagctc ccagaggcga caatgaccat 4381 ccttaaagct gcagctgacc cagccctaag cacagacttt cagaccattt tggacatcag 4441 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc 4501 catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca 4561 cctgtaggtt tggcaagcta gctggccaga catgataaga tacattgatg agtttggaca 4621 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 4681 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 4741 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa 4801 atgtggtatg gaattggagc cccactgtgt tcatcttaca gatggaaata ctgacattca 4861 gaggagttag ttaacttgcc tagcggccgc tgcattctag ttgtggtttg tccaaactca 4921 tcaatgtatc ttatcatgtc tggatcccct tgaaatacat ccacaggtac acctccaatt 4981 gactcaaatg atgtcaatta gtctatcaga agcttctaaa gccatgacat cattttctgg 5041 aattttccaa gctgtttaaa ggcacagtca acttagtgta tgtaaacttc tgacccactg 5101 gaattgtgat acagtgaatt ataagtgaaa taatctgtct gtaaacaatt gttggaaaaa 5161 tgacttgtgt catgcacaaa gtagatgtcc taactgactt gccaaaacta ttgtttgtta 5221 acaagaaatt tgtggagtag ttgaaaaacg agttttaatg actccaactt aagtgtatgt 5281 aaacttccga cttcaactgt atatctagag tcgacctgca ggcatgcaag cttggcgtaa 5341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 5401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 5461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 5521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 5581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5941 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 6001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 6061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 6121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 6181 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 6241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 6301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 6601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 7021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 7081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 7141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 7201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 7261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 7321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 7381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 7441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 7501 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 7561 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 7621 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 7681 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg 7741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 7801 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 7861 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 7921 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt //