LOCUS       pSB-CMV-MCS-Puro       11236 bp    DNA     circular      8-FEB-2014
SOURCE      
  ORGANISM  
COMMENT     izraeli csoporttol, eredeti nev: pT2-HB-CAG-AMAXA
            http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|689186991|
COMMENT     VNTDBDATE|689186991|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSB-CMV-MCS-Puro cPLA2-mKate2-P2A-EGFP-LMNB1|
COMMENT     VNTAUTHORNAME|Enyedi Balázs|
COMMENT     VNTOAUTHORNAME|Eszter&Tamas|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pSB-CMV-MCS-Puro cPLA2-mKate2-P2A-EGFP-LMNB1" 0 11236 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AatII" "AgeI" "ApaI" "BamHI" "BglII" "BsaBI" "BstXI" 
COMMENT         "Bsu36I" "ClaI" "EcoRI" "EcoRV" "HindIII" "KpnI" "MfeI" "MluI" "NcoI" 
COMMENT         "NheI" "NotI" "PstI" "PvuI" "SacI" "SacII" "SalI" "SfoI" "SmaI" "SpeI" 
COMMENT         "SphI" "SspI" "XbaI" "XhoI" "BsrGI" "BspEI" "BstBI") 
COMMENT        (CStringList "atg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 11236 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT            1721 0 5576 0 6944 0 7442 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ApaI" "gggccc" 6 0 3 2487 0 6254 0 8076 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 5 705 0 942 0 3748 0 8041 
COMMENT            0 10943 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BglII" "agatct" 2 0 1 4471 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 947 0 7730 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 963 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 2 3239 0 7494 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "ClaI" "atcgat" 3 0 3 713 0 5810 0 7724 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2957 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "HindIII" "aagctt" 2 0 4 2 0 687 0 1216 0 5614 
COMMENT             0 0 "") 
COMMENT        #12=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 697 0 8082 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "MfeI" "caattg" 2 0 2 7630 0 11102 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 386 0 3752 0 6761 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 671 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "PstI" "ctgcag" 6 0 5 2052 0 4589 0 4979 0 
COMMENT             5411 0 6126 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 9739 0 10784 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "SacI" "gagctc" 6 0 8 594 0 703 0 1472 0 1648 
COMMENT             0 1730 0 4703 0 7509 0 10941 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 4524 0 7112 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 2398 0 7006 0 8057 0 0 
COMMENT             "") 
COMMENT        #23=(CRSite (CStringList) "SfoI" "ggcgcc" 4 0 2 7107 0 7238 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 6949 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 25 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 6481 0 6602 0 6674 0 0 
COMMENT             "") 
COMMENT        #27=(CRSite (CStringList) "SspI" "aatatt" 4 0 3 1024 0 10173 0 10726 0 0 
COMMENT             "") #28=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 6244 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 3 2657 0 7502 0 8063 0 0 
COMMENT             "") 
COMMENT        #30=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 3023 0 4463 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BspEI" "tccgga" 2 0 2 2277 0 2630 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 2521 0 0 "")) 
COMMENT       (CObList 
COMMENT        #33=(CFSignal (CObList) "ori" 33 0 0 8354 9234 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5803..6683") 
COMMENT        #34=(CFSignal (CObList) "Amp" 4 0 1 9298 10155 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6747..7604)") 
COMMENT        #35=(CFSignal (CObList) "F1 ori" 33 0 0 10286 10742 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7735..8191") 
COMMENT        #36=(CFSignal (CObList) "T3" 30 0 1 8094 8113 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5543..5562)") 
COMMENT        #37=(CFSignal (CObList) "T7" 30 0 0 10908 10928 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "8357..8377") 
COMMENT        #38=(CFSignal (CObList) "IR-DR(L)" 44 0 1 10953 11179 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(8402..8628)") 
COMMENT        #39=(CFSignal (CObList) "IR-DR(R)" 44 0 0 7807 8034 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5256..5483") 
COMMENT        #40=(CFSignal (CObList) "polyA" 25 0 0 7609 7614 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5058..5063") 
COMMENT        #41=(CFSignal (CObList) "BGH pA" 26 0 0 6280 6504 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1870000\"") 1 1 1 1 "3729..3953") 
COMMENT        #42=(CFSignal (CObList) "BGH reverse primer" 27 0 1 6274 6291 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1910000\"") 1 1 1 1 
COMMENT             "complement(3723..3740)") 
COMMENT        #43=(CFSignal (CObList) "CMV forward primer" 27 0 0 544 564 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1930000\"") 1 1 1 1 
COMMENT             "544..564") 
COMMENT        #44=(CFSignal (CObList) "CMV promoter" 29 0 0 7 594 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2180000\"") 1 1 1 1 "7..594") 
COMMENT        #45=(CFSignal (CObList) "T7 primer" 27 0 0 638 657 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2100000\"") 1 1 1 1 "638..657") 
COMMENT        #46=(CFSignal (CObList) "T7 promoter" 30 0 0 638 654 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2370000\"") 1 1 1 1 "638..654") 
COMMENT        #47=(CFSignal (CObList) "pBABE_3_primer" 21 0 1 6534 6554 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3983..4003)") 
COMMENT        #48=(CFSignal (CObList) "SV40_enhancer" 21 0 1 6540 6755 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3989..4204)") 
COMMENT        #49=(CFSignal (CObList) "SV40_promoter" 30 0 0 6552 6820 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4001..4269") 
COMMENT        #50=(CFSignal (CObList) "SV40_origin" 33 0 0 6719 6796 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4168..4245") 
COMMENT        #51=(CFSignal (CObList) "SV40_promoter" 30 0 0 6672 6874 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4121..4323") 
COMMENT        #52=(CFSignal (CObList) "SV40pro_F_primer" 21 0 0 6781 6800 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4230..4249") 
COMMENT        #53=(CFSignal (CObList) "puro(variant)" 4 0 0 6897 7496 0 (CStringList) 
COMMENT             (CStringList "/gene=\"puro(variant)\"") 1 1 1 1 "4346..4945") 
COMMENT        #54=(CFSignal (CObList) "cPLA2" 21 0 0 723 2946 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "948..3171") 
COMMENT        #55=(CFSignal (CObList) "PCR mut A-->G" 21 0 0 923 923 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1148..1148") 
COMMENT        #56=(CFSignal (CObList) "mK2" 21 0 0 2973 3680 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3198..3905") 
COMMENT        #57=(CFSignal (CObList) "P2A peptide" 21 0 0 3681 3746 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3906..3971") 
COMMENT        #58=(CFSignal (CObList) "EGFP" 21 0 0 3753 4469 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3978..4694") 
COMMENT        #59=(CFSignal (CObList) "LMNB1" 21 0 0 4476 6242 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4701..6467") 
COMMENT        #60=(CFSignal (CObList) "cPLA2-mKate2-P2A-EGFP-LMNB1" 21 0 0 723 6242 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "948..6467")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA Plasmid 'pSB-CMV-MCS-Puro cPLA2-mKate2-P2A-EGFP-LMNB1'" 
COMMENT                          1) 
COMMENT                     #65=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Eszter&Tamas" 
COMMENT                          1) 
COMMENT                     #66=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/08/14 04:49PM" 1) 
COMMENT                     #67=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/08/14 04:49PM" 1) 
COMMENT                     #68=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 11236 bp" 1) 
COMMENT                     #69=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #70=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #73=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 27-JAN-2014" 1) 
COMMENT                     #74=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  puro(variant)" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #75=(CFolderPar 
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COMMENT                   "Annotations") 
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COMMENT                                        " N3: 287 " 1) 
COMMENT                                   #82=(CLinePar 
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COMMENT                                        " N4: 473 " 1) 
COMMENT                                   #83=(CLinePar 
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COMMENT                                   #84=(CLinePar 
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COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 8) 1 2 2 0 191) 
COMMENT                                        " N8: 7442 " 1))))) "AatII: 8 sites") 5 
COMMENT                          1 "GACGTC" "CTGCAG") 
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COMMENT                           (CGroupPar (CParagraph 65360528 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "AgeI: 0 sites") 1 5 "ACCGGT" 
COMMENT                          "TGGCCA") 
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COMMENT                                   #92=(CLinePar 
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COMMENT                          "GGGCCC" "CCCGGG") 
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COMMENT                                   #96=(CLinePar 
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COMMENT                                   #97=(CLinePar 
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COMMENT                                   #99=(CLinePar 
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COMMENT                          5 "GGATCC" "CCTAGG") 
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COMMENT                                     #106=(CLinePar 
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COMMENT                           5 5 "GATNNNNATC" "CTANNNNTAG") 
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COMMENT                           1 5 "TCCGGA" "AGGCCT") 
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COMMENT                           1 5 "TGTACA" "ACATGT") 
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COMMENT                           2 4 "TTCGAA" "AAGCTT") 
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COMMENT                           4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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COMMENT                           1 5 "GAATTC" "CTTAAG") 
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COMMENT       (CSeqView 10 10 (CObjectList #654=(XLATFRAGMENT 722 1839 0 1 723 5519)) 
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COMMENT       22520156 (CStringList) 22520216 22520192 
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COMMENT        #663=(CSampFrag "pSB-CMV-MCS-Puro drcPLA2-mKate2" 5573 671 "NheI" 6244 
COMMENT              "XbaI" (LOGPEN 0 0 0) 1.47673e-316 1.12637e-289) 
COMMENT        #664=(CSampFrag "pSB-CMV-MCS-Puro drcPLA2-mKate2" 3472 7630 "MfeI" 11102 
COMMENT              "MfeI" (LOGPEN 0 0 0) 3.30268e-316 2.5779e-312) 
COMMENT        #665=(CSampFrag "pSB-CMV-MCS-Puro drcPLA2-mKate2" 1386 6244 "XbaI" 7630 
COMMENT              "MfeI" (LOGPEN 0 0 0) 1.47673e-316 1.12926e-289) 
COMMENT        #666=(CSampFrag "pSB-CMV-MCS-Puro drcPLA2-mKate2" 805 11102 "MfeI" 671 
COMMENT              "NheI" (LOGPEN 0 0 0) 1.6976e-313 1.12348e-289))))
FEATURES             Location/Qualifiers
     rep_origin      8354..9234
                     /vntifkey="33"
                     /label=ori
     CDS             complement(9298..10155)
                     /vntifkey="4"
                     /label=Amp
     rep_origin      10286..10742
                     /vntifkey="33"
                     /label=F1\ori
     promoter        complement(8094..8113)
                     /vntifkey="30"
                     /label=T3
     promoter        10908..10928
                     /vntifkey="30"
                     /label=T7
     transposon      complement(10953..11179)
                     /vntifkey="44"
                     /label=IR-DR(L)
     transposon      7807..8034
                     /vntifkey="44"
                     /label=IR-DR(R)
     polyA_signal    7609..7614
                     /vntifkey="25"
                     /label=polyA
     polyA_site      6280..6504
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     primer          complement(6274..6291)
                     /invitrogen="1910000"
                     /vntifkey="27"
                     /label=BGH\reverse\primer
     primer          544..564
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        7..594
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     primer          638..657
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        638..654
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    complement(6534..6554)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(6540..6755)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        6552..6820
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      6719..6796
                     /vntifkey="33"
                     /label=SV40_origin
     promoter        6672..6874
                     /vntifkey="30"
                     /label=SV40_promoter
     misc_feature    6781..6800
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             6897..7496
                     /gene="puro(variant)"
                     /vntifkey="4"
                     /label=puro(variant)
     misc_feature    723..2946
                     /vntifkey="21"
                     /label=cPLA2
     misc_feature    923..923
                     /vntifkey="21"
                     /label=PCR\mut\A-->G
     misc_feature    2973..3680
                     /vntifkey="21"
                     /label=mK2
     misc_feature    3681..3746
                     /vntifkey="21"
                     /label=P2A\peptide
     misc_feature    3753..4469
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    4476..6242
                     /vntifkey="21"
                     /label=LMNB1
     misc_feature    723..6242
                     /vntifkey="21"
                     /label=cPLA2-mKate2-P2A-EGFP-LMNB1
BASE COUNT     2907 a      2875 c      2901 g      2553 t 
ORIGIN
        1 aagcttgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 
      661 cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatccc atcgatgcca 
      721 caatgtccaa cattatagtt gagcatcagt attctcatag actgaaattg actgtggtga 
      781 gagctgaaaa tgtaactaaa ggagcatttg gggatttatt ggacaccccg gacccttatg 
      841 ttgagctgtc agtgccaacc acaccagagt caagaaaacg aacccgccac attaacaatg 
      901 acattaaccc caagtggaac gagacctttg agttcatatt ggatcccaat caatccaatg 
      961 ttttggaggt gacactgatg gatgccaatt atgtcatgga tgaaactctt ggcacagcaa 
     1021 aatattccct ctcaaaactc aaagtggcac aaatggagca tgtgaccctc tctatcggca 
     1081 agacaaccaa agtgttcctc gacttgctct tggaagtttg cgcatccaca gacctgcgct 
     1141 tcagcatgac actgtgtgat caagagaagc tgttcatgca gacacgcaga gacagggtca 
     1201 tgctcagcat aaagaagctt cttaaaatgg aaaaccctcg tttccttcca tcctcaccaa 
     1261 gagaggttcc caccatcgct attctgggct ctggaggagg ttttcgtgca atggtgggct 
     1321 tctctggtgt gatgaaggct ttgtatgaat ctggcgtgtt tgactgtgca acatatgtgg 
     1381 caggactttc aggatcaaca tggtatatgt ctatgctgta ctctcatcca gagtttcctg 
     1441 ctaaaggccc aggcgacatt aataaagagc tcatgaacag agtcagtaat aacccactca 
     1501 aactgctcct gccccaaaac atcaaccgct acgtcaaagc actgtggaag aagaaatctg 
     1561 ctggacagcc ggtcaccttc actgacatct tcgggatgtt gataggagag acgcttattc 
     1621 cagggagaat gaacattaag ctgagctcat tgaaagggaa aattaatgaa ggtcagagcc 
     1681 ctcttcctct cttcacctgt ctgcacgtca aacctgacgt ctctgagctc atgtttgcag 
     1741 attgggtgga gttcagccct tatgaaattg gcatggccaa atatggcacc ttcatgtctc 
     1801 ctgggctgtt tggcagcaaa ttcttcatgg gaagtgttgt gaaacaatat gaggaaaacc 
     1861 ccttacattt cctgatgggt gtgtggggaa gtgctttttc catcctcttt aaccgggttt 
     1921 tgggtgtgaa ggagacgacc agcagtagca ctatggagga ggagctggag caaattaaac 
     1981 cagagcacat tgtgggtgac gattctgctg ataacgagga ggaaactcaa agaggaggca 
     2041 cagagtctgc agatgcagag gacgagcgcc agcgtcatgc ccaggccagc tgggtacagc 
     2101 gcatgttgac ctccatcatg ggcgacacca ctctgttcac cactcgtgag ggccgcgcag 
     2161 gcaaagtgca caacttcatg ctgggactga acctgaactc aaccctgccc ttctctccct 
     2221 tcagcggcat cacacaccag acctcgctgg aggaggaagt ggacgctgtt actgatccgg 
     2281 atgagtttga gcgaatctat gagcctctgg atgtgaagag caagaaaatc catgtggtgg 
     2341 acagtggctt gacctttaac ctgccctacc ctctcattct gcggtgtcag agaggagtcg 
     2401 acctgattat ttcattcgac ttctcagccc gacccagtga ctccagccca ccgttcaaag 
     2461 agttactgct ggctgaaaaa tgggcccgta tgaataagct gcccttcccg aagattgatt 
     2521 cgaaagtatt cgacagagag ggtctaaaag agtgctatgt gttcaagcct gctaaaggag 
     2581 acaagaactg ccccaccatc attcattttg tcttggccaa catcaacttc cggaatttta 
     2641 aagcccctgg agttcctcga gattcagaca aggacataga gtttggtgac tttgacatat 
     2701 ttgacgagcc agcctcccct tactccactt tcaacttcaa atacaacaac caggccttca 
     2761 agagacttca tgacctcatg gagttcaata cgcttaacaa catcgaggtt atcaaagaag 
     2821 ccattaaaga cagcattctg ctgagacgag agaacccagc gcgatgttcg gtttcccttt 
     2881 cgctcagtga aatcgaaaac aagaagttcc tgaaacgaga caacagcatt gcaaaacgac 
     2941 ccaccggagc tggaggaatt cgtcctggat ctatggtgtc taagggcgaa gagctgatta 
     3001 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt 
     3061 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggccg 
     3121 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca 
     3181 gcaaaacctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg 
     3241 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc 
     3301 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact 
     3361 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga 
     3421 ccctgtaccc cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg 
     3481 gcgggggcca cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga 
     3541 acctcaagat gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg 
     3601 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta 
     3661 gcaaactggg gcacaaactg ggaagcggag ctactaactt cagcctgctg aagcaggctg 
     3721 gagacgtgga ggagaaccct ggacctggat ccatggtgag caagggcgag gagctgttca 
     3781 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 
     3841 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 
     3901 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 
     3961 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 
     4021 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 
     4081 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 
     4141 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 
     4201 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 
     4261 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 
     4321 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 
     4381 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 
     4441 tcactctcgg catggacgag ctgtacaaga gatctatggc gagcgcggcg tgtactccgg 
     4501 ccgggcagca gtcgttgtcc cgcggcacca gcagcactcc actgagcccc gcgcggatct 
     4561 ccaggctgca agagaaagag gagctgcagc agctcaatga ccggcttgcc gcctacatcg 
     4621 acacagtccg tagtctggag tccgagaaca gcgtcctgca aatacagatc agagaacgag 
     4681 aggatgtgcg gaaccgcgag ctcacggggc tcagaacact ctacgaaacc gagctggcgg 
     4741 acgcacggaa ggcgcttgat gacacggcac gggagcgcgc acgattgcag attgaactgg 
     4801 gcaagattaa aagcgagcaa gagcagctac agcacacata tgccaagaaa gatgctgacc 
     4861 tcaccagctc tcagaaccga ctaaaagatg ttgaagccct gttgaacgct aaagaagctg 
     4921 cactaaacac agccgtctct gaaaggaaga ctctggaaaa cactctatct gatctgcaga 
     4981 tccgtgtcca ggagttggaa ggagggttga tctccgctaa gaagcagctg tcagatgaga 
     5041 ccctgctgcg tgtggatctg gagaaccgct gtcagagcct tatggaggaa cttgaattta 
     5101 ggaaaaacat gtttgaagag gaaatcaaag atacacgtcg tagacatgag acccgtcttg 
     5161 tcgaggtgga ttcaggtcgt cagatggagt atgagtttaa actggctcag gctctgactg 
     5221 acatgagaca gcagcatgat gagcaagtca aactttataa ggaggagatg gagcagacat 
     5281 acgtgtccaa gctggagaac attcgtttgt catctgagat gaacagctct tcagctagta 
     5341 tggcccgtga ggagcttcga gagtctacac tgagagttga gagtttggca ggtcaacttg 
     5401 caaatctgca gaaagaggct cgtgcctggc aggatcgcat taatgaactg gagggggctc 
     5461 ttagccatga gaaagattta agccgtaggt tgctggcaga gaaagagcgc gagattgctg 
     5521 agatcagagc caagatgcag cagcagctgg atgagtatga gcaactgctg gacgtcaaac 
     5581 tagcgctgga catggagatc aatgcttaca gaaagcttct ggagggagaa gaagaaaggt 
     5641 tgaaactgtc tccaagtcct tcatctcgtg tgactgtgtc acgagcatcc tccagtcgca 
     5701 gtgttcgtac tacccgtggc aagagaaagc gtgtggatgt ggaggagtcg gaggcaagca 
     5761 gcagtgtcag tatcgctcat tctgcatctg ccactggaaa cgtctccatc gatgagctgg 
     5821 atgtagatgg gaagttcatc cgcttgcaca acaactctga gcaggaccag ccgatggcag 
     5881 gatttcagct gaccaggact attggagaag ttactgcaac atataaattc actgcaaaat 
     5941 acaatctgaa ggctggacag aaagtgacga tctgggcatc taatgcaggc ataagctcca 
     6001 accctcctgc tgacctgata tggaagaacc agccttcatg ggggactggg gagaacataa 
     6061 aagtggctct tttgagccca gcaggagagg aagtagctgt gagaagcaca gtattcaaga 
     6121 ctgcagtgga agatgaagac gacgatgagg aggaagaaaa ggaagctgaa gtcattgagg 
     6181 agcatttcta tcaccagcag ggagatccac gcgctgctaa gagaggatgt ttgatcatgt 
     6241 aatctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 
     6301 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 
     6361 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 
     6421 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 
     6481 ctggggatgc ggtgggctct atggaccgga ctgaggcgga aagaaccagc tgtggaatgt 
     6541 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     6601 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 
     6661 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 
     6721 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 
     6781 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 
     6841 cttttttgga ggcctaggct tttgcaaaaa gctagatttt tctgacccta gccaccatga 
     6901 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca 
     6961 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc 
     7021 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 
     7081 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 
     7141 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 
     7201 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 
     7261 cgtggttcct ggccaccgtc ggcgtgtcgc ccgaccacca gggcaagggt ctgggcagcg 
     7321 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 
     7381 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 
     7441 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggat 
     7501 ctcgagctcg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 
     7561 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 
     7621 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 
     7681 taaagcaagt aaaacctcta caaatgtggt acttaagagg gatcgataag gatctagctt 
     7741 gtggaaggct actcgaaatg tttgacccaa gttaaacaat ttaaaggcaa tgctaccaaa 
     7801 tactaattga gtgtatgtaa acttctgacc cactgggaat gtgatgaaag aaataaaagc 
     7861 tgaaatgaat cattctctct actattattc tgatatttca cattcttaaa ataaagtggt 
     7921 gatcctaact gacctaagac agggaatttt tactaggatt aaatgtcagg aattgtgaaa 
     7981 aagtgagttt aaatgtattt ggctaaggtg tatgtaaact tccgacttca actgtatagg 
     8041 gatcctctag ctagagtcga cctcgagggg gggcccggta cccagctttt gttcccttta 
     8101 gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     8161 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     8221 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     8281 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     8341 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     8401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     8461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     8521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     8581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     8641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     8701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     8761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     8821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     8881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     8941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     9001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     9061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     9121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     9181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     9241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     9301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     9361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     9421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     9481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     9541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     9601 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     9661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     9721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     9781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     9841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     9901 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     9961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
    10021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
    10081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
    10141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
    10201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
    10261 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg 
    10321 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
    10381 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
    10441 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
    10501 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
    10561 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
    10621 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 
    10681 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 
    10741 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 
    10801 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 
    10861 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca 
    10921 ctatagggcg aattggagct cggatcccta tacagttgaa gtcggaagtt tacatacact 
    10981 taagttggag tcattaaaac tcgtttttca actactccac aaatttcttg ttaacaaaca 
    11041 atagttttgg caagtcagtt aggacatcta ctttgtgcat gacacaagtc atttttccaa 
    11101 caattgttta cagacagatt atttcactta taattcactg tatcacaatt ccagtgggtc 
    11161 agaagtttac atacactaag ttgactgtgc ctttaaacag cttggaaaat tccagaaaat 
    11221 gatgtcatgg ctttag 
//
