LOCUS       pARC8_Ec_SSAP_Ec        4642 bp    DNA     circular UNA 23-OCT-2020
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...b-5nyi18k
VERSION     urn.local...b-5nyi18k
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        24..153
                     /standard_name="promoter"
     source          complement(<162..>947)
                     /organism="Escherichia virus Lambda"
                     /mol_type="genomic DNA"
                     /host="Escherichia coli"
                     /db_xref="taxon:10710"
     CDS             162..947
                     /gene="bet"
                     /locus_tag="lambdap84"
                     /codon_start=1
                     /transl_table=11
                     /product="bet"
                     /protein_id="NP_040617.1"
                     /db_xref="GeneID:3827055"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
                     /standard_name="bet CDS"
     gene            162..947
                     /gene="bet"
                     /locus_tag="lambdap84"
                     /db_xref="GeneID:3827055"
                     /standard_name="bet gene"
     CDS             944..>947
                     /gene="exo"
                     /locus_tag="lambdap41"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="NP_040616.1"
                     /db_xref="GeneID:2703522"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIG
                     FVFGEQWR"
                     /standard_name="exo CDS"
     gene            944..>947
                     /gene="exo"
                     /locus_tag="lambdap41"
                     /db_xref="GeneID:2703522"
                     /standard_name="exo gene"
     misc_feature    945..1505
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAATAAGGAGGAAAAACATATGGCGATCGGTGACACCAACA
                     TCACCGTTGTTGGTAACATCGTTGCGGACCCGGAACTGCNTTTCNCCCCGTCTGGTGC
                     GGCGGTTGCGAACTTCCGTATCGCGTCTACCCCGCGTTCTTTCAACCGTCAGACCAAC
                     CAGTGGGAAGACGGTGAAGCGCTGTTCCTGACCGTTAACGTTTGGCGTCAGGCGGCGG
                     AAAACGTTGCGGAATCTCTGTCTAAAGGTATGCGTGTTATCGTTACCGGTCGTCTGAA
                     ACAGCGTTCTTACGAAACCCGTGAAGGTGAAAAACGTTCTGTTTTCGAAGTTGAAGCG
                     GACGAAGTTGGTCCGTCTCTGACCTTCGCGAAAGCGGACGTTCAGCGTACCCCGCGTG
                     GTGGTAACTCTGGTGGTAACTACGGTGGTGGTAACCAGGGTGGTGGTCTGGGTGGTAA
                     CCAGGGTAACCAGCAGGGTGGTTTCTCTAACCAGAACTCTGGTGGTTTCGGTGGTAAC
                     CAGGGTAACCAGCAGCAGTCTAACCAGGGTGGTTTCGGTGGTAACCAGAACCAGTCTC
                     AGGGTAACAACTTCAACCAGGGTGGTTTCGGTGGTGGTTCTCCGCAAGCTGCACCTGA
                     CAACGACCCGTGGAACTCTGCTCCACCTGCGGGTTCTGGTGGTTNCGGNNNNNNGGAC
                     GACGAACCGCCGTTCTAATG"
     primer_bind     948..968
                     /standard_name="CB2"
     source          <969..>1505
                     /sub_strain="MG1655"
                     /strain="K-12"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /db_xref="taxon:511145"
     CDS             969..1505
                     /function="factor; DNA - replication, repair,
                     restriction/modification"
                     /locus_tag="b4059"
                     /codon_start=1
                     /gene_synonym="ECK4051; exrB; JW4020; lexC"
                     /transl_table=11
                     /note="ssDNA-binding protein"
                     /db_xref="GI:16131885"
                     /db_xref="ASAP:ABE-0013298"
                     /db_xref="UniProtKB/Swiss-Prot:P0AGE0"
                     /db_xref="EcoGene:EG10976"
                     /db_xref="GeneID:948570"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
                     /gene="ssb"
                     /product="single-stranded DNA-binding protein"
                     /protein_id="NP_418483.1"
                     /standard_name="ssb CDS"
     gene            969..1505
                     /db_xref="EcoGene:EG10976"
                     /db_xref="GeneID:948570"
                     /locus_tag="b4059"
                     /gene="ssb"
                     /gene_synonym="ECK4051; exrB; JW4020; lexC"
                     /standard_name="ssb gene"
     mat_peptide     972..1502
                     /product="single-stranded DNA-binding protein"
                     /locus_tag="b4059"
                     /gene="ssb"
                     /gene_synonym="ECK4051; exrB; JW4020; lexC"
                     /experiment="N-terminus verified by Edman degradation:
                     PMID 6270666,7042342"
                     /standard_name="ssb peptide"
     rep_origin      1552..2464
     gene            2826..3485
                     /standard_name="Beta Lactamase"
     CDS             complement(3621..4463)
                     /standard_name="AraC protein"
     mRNA            complement(4464..4618)
                     /standard_name="mRNA"
     regulatory      4493..153
                     /standard_name="regulatory"
ORIGIN      
        1 ctataactat ggctggaatg tccacattga atatttgcac agcgtcacac tttgcaaagc
       61 attagcattt ttgtccataa gattagcgga tcctgcctga cggtttttgc cgcgactctc
      121 tataatttct ccatacctgt ttttctggat ggagtaagac catgagtact gcactcgcaa
      181 cgctggctgg gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga
      241 tcaccactct tcgccagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat
      301 tactgatcgt tgccaaccag tacggcctta atccgtggac gaaagaaatt tacgcctttc
      361 ctgataagca gaatggcatc gttccggtgg tgggcgttga tggctggtcc cgcatcatca
      421 atgaaaacca gcagtttgat ggcatggact ttgagcagga caatgaatcc tgtacatgcc
      481 ggatttaccg caaggaccgt aatcatccga tctgcgttac cgaatggatg gatgaatgcc
      541 gccgcgaacc attcaaaact cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc
      601 ccaaacggat gttacgtcat aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg
      661 ctggtatcta tgacaaggat gaagccgagc gcattgtcga aaatactgca tacactgcag
      721 aacgtcagcc ggaacgcgac atcactccgg ttaacgatga aaccatgcag gagattaaca
      781 ctctgctgat cgccctggat aaaacatggg atgacgactt attgccgctc tgttcccaga
      841 tatttcgccg cgacattcgt gcatcgtcag aactgacaca ggccgaagca gtaaaagctc
      901 ttggattcct gaaacagaaa gccgcagagc agaaggtggc agcatgaaaa aaataggagg
      961 aaaaacatat ggccagcaga ggcgtaaaca aggttattct cgttggtaat ctgggtcagg
     1021 acccggaagt acgctacatg ccaaatggtg gcgcagttgc caacattacg ctggctactt
     1081 ccgaatcctg gcgtgataaa gcgaccggcg agatgaaaga acagactgaa tggcaccgcg
     1141 ttgtgctgtt cggcaaactg gcagaagtgg cgagcgaata tctgcgtaaa ggttctcagg
     1201 tttatatcga aggtcagctg cgtacccgta aatggaccga tcaatccggt caggatcgct
     1261 acaccacaga agtcgtggtg aacgttggcg gcaccatgca gatgctgggt ggtcgtcagg
     1321 gtggtggcgc tccggcaggt ggcaatatcg gtggtggtca gccgcagggc ggttggggtc
     1381 agcctcagca gccgcagggt ggcaatcagt tcagcggcgg cgcgcagtct cgcccgcagc
     1441 agtccgctcc ggcagcgccg tctaacgagc cgccgatgga ctttgatgat gacattccgt
     1501 tctgaactag tggggaagct tatcgatgat aagctgtcaa acatgagaat tacaacttat
     1561 atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga
     1621 aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct
     1681 cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta
     1741 ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt
     1801 cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg
     1861 gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg
     1921 gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     1981 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     2041 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccaggggg
     2101 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     2161 ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc
     2221 acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc
     2281 atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     2341 tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat
     2401 gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta
     2461 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga
     2521 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
     2581 tcagtaagtt ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag
     2641 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     2701 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     2761 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     2821 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     2881 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     2941 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     3001 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     3061 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     3121 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     3181 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     3241 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     3301 acgtggccaa tatggacaac ttcttcgccc ccgttttcac tatgggcaaa tattatacgc
     3361 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     3421 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     3481 cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa
     3541 gtgataataa gcggatgaat ggcagaaatt cgaatagtta cggcttatga catctttgtg
     3601 gacacatcat tcacttttta ttcacatccg gccctgaact cgctaggact tgccccggtg
     3661 cattttttaa atacccgcga aaaatagagc tgatcgtcaa atccaacatt gcgcccaacg
     3721 gtcgctatcg gcattcgcgt agtgctaagc agaagtttcg cctggctgat acgctgatct
     3781 tcgcgccagc tcaatacgct aatgcctaac tgctggcgga acagatgtga taaccgggag
     3841 ggcgacaggc agacatgctg ggcgacgctg gcgatatcaa aatggctgtc cgccagatgg
     3901 tcgctgatat actggcaggc atcgcgcaca cggctatcca tcggcgggtg caacgactca
     3961 ttaattaccg ccatacgtct gagcaacaac tgctccagca gattgatcgc cagtagctca
     4021 gaatagcgac cttccccttg cccggcgctg atgatctgcc cgaacagttc gctgaaatgc
     4081 ggctggcgcg cctcgtccgg gcggaaaaat cctgtctggg caaagattgt cggccaggtc
     4141 agccactcct gccagtaggc gcgaggccgg aaataaaccc actggtgata ccactcgctg
     4201 gcgtccggat gccgtccata gtgatgaatc tcgcccggcg gaaacaataa tatatcgcca
     4261 ggccgacaga caaactgctc gccattatta ttaatgacgc cctctccgcg gatggtcagg
     4321 ttaagaatat atcccttcat gcccaacgga cgatcgataa aaaaatccag atatccattc
     4381 gcttcgatcg gcgtcagccc ggcgaccaga tgggcattaa atgaatatcc cggcaatagc
     4441 ggatcatttt gcgtttcagc catgatttct ctaccccccg atgttcagag aagaaacaaa
     4501 ttgtccatat cgaccaggac gacagagctt ccgtctccgc aagactttgc gcttgatgaa
     4561 agcacgtatc aaccccgctt gtgaaaagcg ctttgtaaca aaagcgtaca gttcaggcga
     4621 taaaattaag taacagaagt gt
//
