LOCUS       pARC8_Ec_SSAP_Ec        6507 bp    DNA     circular UNA 23-OCT-2020
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...b-5nyi18k
VERSION     urn.local...b-5nyi18k
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        24..153
                     /standard_name="promoter"
     source          complement(<162..>947)
                     /organism="Escherichia virus Lambda"
                     /mol_type="genomic DNA"
                     /host="Escherichia coli"
                     /db_xref="taxon:10710"
     CDS             162..947
                     /gene="bet"
                     /locus_tag="lambdap84"
                     /codon_start=1
                     /transl_table=11
                     /product="bet"
                     /protein_id="NP_040617.1"
                     /db_xref="GeneID:3827055"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
                     /standard_name="bet CDS"
     gene            162..947
                     /gene="bet"
                     /locus_tag="lambdap84"
                     /db_xref="GeneID:3827055"
                     /standard_name="bet gene"
     misc_feature    945..3370
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAATAAGGAGGAAAAACATATGGCGATCGGTGACACCAACA
                     TCACCGTTGTTGGTAACATCGTTGCGGACCCGGAACTGCNTTTCNCCCCGTCTGGTGC
                     GGCGGTTGCGAACTTCCGTATCGCGTCTACCCCGCGTTCTTTCAACCGTCAGACCAAC
                     CAGTGGGAAGACGGTGAAGCGCTGTTCCTGACCGTTAACGTTTGGCGTCAGGCGGCGG
                     AAAACGTTGCGGAATCTCTGTCTAAAGGTATGCGTGTTATCGTTACCGGTCGTCTGAA
                     ACAGCGTTCTTACGAAACCCGTGAAGGTGAAAAACGTTCTGTTTTCGAAGTTGAAGCG
                     GACGAAGTTGGTCCGTCTCTGACCTTCGCGAAAGCGGACGTTCAGCGTACCCCGCGTG
                     GTGGTAACTCTGGTGGTAACTACGGTGGTGGTAACCAGGGTGGTGGTCTGGGTGGTAA
                     CCAGGGTAACCAGCAGGGTGGTTTCTCTAACCAGAACTCTGGTGGTTTCGGTGGTAAC
                     CAGGGTAACCAGCAGCAGTCTAACCAGGGTGGTTTCGGTGGTAACCAGAACCAGTCTC
                     AGGGTAACAACTTCAACCAGGGTGGTTTCGGTGGTGGTTCTCCGCAAGCTGCACCTGA
                     CAACGACCCGTGGAACTCTGCTCCACCTGCGGGTTCTGGTGGTTNCGGNNNNNNGGAC
                     GACGAACCGCCGTTCTAATG"
     source          <969..>1505
                     /sub_strain="MG1655"
                     /strain="K-12"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /db_xref="taxon:511145"
     CDS             969..1505
                     /function="factor; DNA - replication, repair,
                     restriction/modification"
                     /locus_tag="b4059"
                     /codon_start=1
                     /gene_synonym="ECK4051; exrB; JW4020; lexC"
                     /transl_table=11
                     /note="ssDNA-binding protein"
                     /db_xref="GI:16131885"
                     /db_xref="ASAP:ABE-0013298"
                     /db_xref="UniProtKB/Swiss-Prot:P0AGE0"
                     /db_xref="EcoGene:EG10976"
                     /db_xref="GeneID:948570"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
                     /gene="ssb"
                     /product="single-stranded DNA-binding protein"
                     /protein_id="NP_418483.1"
                     /standard_name="ssb CDS"
     gene            969..1505
                     /db_xref="EcoGene:EG10976"
                     /db_xref="GeneID:948570"
                     /locus_tag="b4059"
                     /gene="ssb"
                     /gene_synonym="ECK4051; exrB; JW4020; lexC"
                     /standard_name="ssb gene"
     mat_peptide     972..1502
                     /product="single-stranded DNA-binding protein"
                     /locus_tag="b4059"
                     /gene="ssb"
                     /gene_synonym="ECK4051; exrB; JW4020; lexC"
                     /experiment="N-terminus verified by Edman degradation:
                     PMID 6270666,7042342"
                     /standard_name="ssb peptide"
     source          complement(<1506..>3370)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1523..3370
                     /standard_name="MutL (E32K)"
     misc_feature    1616..1618
                     /standard_name="E32K"
     rep_origin      3417..4329
     gene            4691..5350
                     /standard_name="Beta Lactamase"
     CDS             complement(5486..6328)
                     /standard_name="AraC protein"
     mRNA            complement(6329..6483)
                     /standard_name="mRNA"
     regulatory      6358..153
                     /standard_name="regulatory"
ORIGIN      
        1 ctataactat ggctggaatg tccacattga atatttgcac agcgtcacac tttgcaaagc
       61 attagcattt ttgtccataa gattagcgga tcctgcctga cggtttttgc cgcgactctc
      121 tataatttct ccatacctgt ttttctggat ggagtaagac catgagtact gcactcgcaa
      181 cgctggctgg gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga
      241 tcaccactct tcgccagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat
      301 tactgatcgt tgccaaccag tacggcctta atccgtggac gaaagaaatt tacgcctttc
      361 ctgataagca gaatggcatc gttccggtgg tgggcgttga tggctggtcc cgcatcatca
      421 atgaaaacca gcagtttgat ggcatggact ttgagcagga caatgaatcc tgtacatgcc
      481 ggatttaccg caaggaccgt aatcatccga tctgcgttac cgaatggatg gatgaatgcc
      541 gccgcgaacc attcaaaact cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc
      601 ccaaacggat gttacgtcat aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg
      661 ctggtatcta tgacaaggat gaagccgagc gcattgtcga aaatactgca tacactgcag
      721 aacgtcagcc ggaacgcgac atcactccgg ttaacgatga aaccatgcag gagattaaca
      781 ctctgctgat cgccctggat aaaacatggg atgacgactt attgccgctc tgttcccaga
      841 tatttcgccg cgacattcgt gcatcgtcag aactgacaca ggccgaagca gtaaaagctc
      901 ttggattcct gaaacagaaa gccgcagagc agaaggtggc agcatgaaaa aaataggagg
      961 aaaaacatat ggccagcaga ggcgtaaaca aggttattct cgttggtaat ctgggtcagg
     1021 acccggaagt acgctacatg ccaaatggtg gcgcagttgc caacattacg ctggctactt
     1081 ccgaatcctg gcgtgataaa gcgaccggcg agatgaaaga acagactgaa tggcaccgcg
     1141 ttgtgctgtt cggcaaactg gcagaagtgg cgagcgaata tctgcgtaaa ggttctcagg
     1201 tttatatcga aggtcagctg cgtacccgta aatggaccga tcaatccggt caggatcgct
     1261 acaccacaga agtcgtggtg aacgttggcg gcaccatgca gatgctgggt ggtcgtcagg
     1321 gtggtggcgc tccggcaggt ggcaatatcg gtggtggtca gccgcagggc ggttggggtc
     1381 agcctcagca gccgcagggt ggcaatcagt tcagcggcgg cgcgcagtct cgcccgcagc
     1441 agtccgctcc ggcagcgccg tctaacgagc cgccgatgga ctttgatgat gacattccgt
     1501 tctgagagag gaggattaca aaatgccaat tcaggtctta ccgccacaac tggcgaacca
     1561 gattgccgca ggtgaggtgg tcgagcgacc tgcgtcggta gtcaaagaac tagtgaaaaa
     1621 cagcctcgat gcaggtgcga cgcgtatcga tattgatatc gaacgcggtg gggcgaaact
     1681 tatccgcatt cgtgataacg gctgcggtat caaaaaagat gagctggcgc tggcgctggc
     1741 tcgtcatgcc accagtaaaa tcgcctctct ggacgatctc gaagccatta tcagcctggg
     1801 ctttcgcggt gaggcgctgg cgagtatcag ttcggtttcc cgcctgacgc tcacttcacg
     1861 caccgcagaa cagcaggaag cctggcaggc ctatgccgaa gggcgcgata tgaacgtgac
     1921 ggtaaaaccg gcggcgcatc ctgtggggac gacgctggag gtgctggatc tgttctacaa
     1981 caccccggcg cggcgcaaat tcctgcgcac cgagaaaacc gaatttaacc acattgatga
     2041 gatcatccgc cgcattgcgc tggcgcgttt cgacgtcacg atcaacctgt cgcataacgg
     2101 taaaattgtg cgtcagtacc gcgcagtgcc ggaaggcggg caaaaagaac ggcgcttagg
     2161 cgcgatttgc ggcaccgctt ttcttgaaca agcgctggcg attgaatggc aacacggcga
     2221 tctcacgcta cgcggctggg tggccgatcc aaatcacacc acgcccgcac tggcagaaat
     2281 tcagtattgc tacgtgaacg gtcgcatgat gcgcgatcgc ctgatcaatc acgcgatccg
     2341 ccaggcctgc gaagacaaac tgggggccga tcagcaaccg gcatttgtgt tgtatctgga
     2401 gatcgaccca catcaggtgg acgtcaacgt gcaccccgcc aaacacgaag tgcgtttcca
     2461 tcagtcgcgt ctggtgcatg attttatcta tcagggcgtg ctgagcgtgc tacaacagca
     2521 actggaaacg ccgctaccgc tggacgatga accccaacct gcaccgcgtt ccattccgga
     2581 aaaccgcgtg gcggcggggc gcaatcactt tgcagaaccg gcagctcgtg agccggtagc
     2641 tccgcgctac actcctgcgc cagcatcagg cagtcgtccg gctgccccct ggccgaatgc
     2701 gcagccaggc taccagaaac agcaaggtga agtgtatcgc cagcttttgc aaacgcccgc
     2761 gccgatgcaa aaattaaaag cgccggaacc gcaggaacct gcacttgcgg cgaacagtca
     2821 gagttttggt cgggtactga ctatcgtcca ttccgactgt gcgttgctgg agcgcgacgg
     2881 caacatttca cttttatcct tgccagtggc agaacgttgg ctgcgtcagg cacaattgac
     2941 gccgggtgaa gcgcccgttt gcgcccagcc gctgctgatt ccgttgcggc taaaagtttc
     3001 tgccgaagaa aaatcggcat tagaaaaagc gcagtctgcc ctggcggaat tgggtattga
     3061 tttccagtca gatgcacagc atgtgaccat cagggcagtg cctttaccct tacgccaaca
     3121 aaatttacaa atcttgattc ctgaactgat aggctacctg gcgaagcagt ccgtattcga
     3181 acctggcaat attgcgcagt ggattgcacg aaatctgatg agcgaacatg cgcagtggtc
     3241 aatggcacag gccataaccc tgctggcgga cgtggaacgg ttatgtccgc aacttgtgaa
     3301 aacgccgccg ggtggtctgt tacaatctgt tgatttacat ccggcgataa aagccctgaa
     3361 agatgagtga actagtgggg aagcttatcg atgataagct gtcaaacatg agaattacaa
     3421 cttatatcgt atggggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat
     3481 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
     3541 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
     3601 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
     3661 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
     3721 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
     3781 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
     3841 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
     3901 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
     3961 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     4021 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
     4081 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
     4141 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
     4201 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
     4261 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc
     4321 cgctagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa
     4381 caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca ccatcataca
     4441 ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac
     4501 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     4561 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     4621 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     4681 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     4741 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     4801 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     4861 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     4921 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     4981 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     5041 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     5101 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     5161 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcactatgg gcaaatatta
     5221 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     5281 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     5341 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
     5401 aataagtgat aataagcgga tgaatggcag aaattcgaat agttacggct tatgacatct
     5461 ttgtggacac atcattcact ttttattcac atccggccct gaactcgcta ggacttgccc
     5521 cggtgcattt tttaaatacc cgcgaaaaat agagctgatc gtcaaatcca acattgcgcc
     5581 caacggtcgc tatcggcatt cgcgtagtgc taagcagaag tttcgcctgg ctgatacgct
     5641 gatcttcgcg ccagctcaat acgctaatgc ctaactgctg gcggaacaga tgtgataacc
     5701 gggagggcga caggcagaca tgctgggcga cgctggcgat atcaaaatgg ctgtccgcca
     5761 gatggtcgct gatatactgg caggcatcgc gcacacggct atccatcggc gggtgcaacg
     5821 actcattaat taccgccata cgtctgagca acaactgctc cagcagattg atcgccagta
     5881 gctcagaata gcgaccttcc ccttgcccgg cgctgatgat ctgcccgaac agttcgctga
     5941 aatgcggctg gcgcgcctcg tccgggcgga aaaatcctgt ctgggcaaag attgtcggcc
     6001 aggtcagcca ctcctgccag taggcgcgag gccggaaata aacccactgg tgataccact
     6061 cgctggcgtc cggatgccgt ccatagtgat gaatctcgcc cggcggaaac aataatatat
     6121 cgccaggccg acagacaaac tgctcgccat tattattaat gacgccctct ccgcggatgg
     6181 tcaggttaag aatatatccc ttcatgccca acggacgatc gataaaaaaa tccagatatc
     6241 cattcgcttc gatcggcgtc agcccggcga ccagatgggc attaaatgaa tatcccggca
     6301 atagcggatc attttgcgtt tcagccatga tttctctacc ccccgatgtt cagagaagaa
     6361 acaaattgtc catatcgacc aggacgacag agcttccgtc tccgcaagac tttgcgcttg
     6421 atgaaagcac gtatcaaccc cgcttgtgaa aagcgctttg taacaaaagc gtacagttca
     6481 ggcgataaaa ttaagtaaca gaagtgt
//
