LOCUS       pCS2plus-kmCerulean156-239-GGGS-Hsa.TDP-43        5610 bp    DNA     circular UNA 17-AUG-2017
DEFINITION  Cloning ligation of kmCerulean156-239-GGGS-Hsa.TDP-43 into
            pCS2plus.
ACCESSION   urn.local...1q-7az4grj
VERSION     urn.local...1q-7az4grj
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        35..52
                     /label="Sp6 promoter"
     misc_feature    80..1634
                     /Source="referenceToDocument: urn:local:.:1f-79uzi1o"
                     /label="kmCerulean156-239-GGGS-Hsa.TDP-43 (concatenated
                     sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <80..84
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        80..83
                     /label="Ligation"
     misc_feature    85..93
                     /ApEinfo_fwdcolor="#fa2536"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="kozak"
     misc_feature    94..348
                     /ApEinfo_fwdcolor="#4370fa"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="User"
                     /label="mCerulean 156 to 239 aa"
     misc_feature    349..384
                     /ApEinfo_fwdcolor="#3d9a3e"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="User"
                     /label="GGS linker"
     misc_feature    385..1629
                     /ApEinfo_fwdcolor="#faf446"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="User"
                     /label="Hsa.TDP-43"
     misc_feature    1630..>1634
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1631..83
                     /Source="referenceToDocument: urn:local:.:6w-79v0f3n"
                     /label="pCS2plus (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1631..1634
                     /label="Ligation"
     polyA_signal    1659..1854
                     /label="SV40 late polyadenylation site"
     polylinker      1855..1887
                     /label="polylinker2"
     misc_feature    1860..1867
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     promoter        4624..5609
                     /label="simian CMV IE94 enhancer/promoter"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccgccgcc accatggaca agcagaagaa cggaatcaag
      121 gccaacttca agatccgcca caacatcgag gacggaagcg tgcagctggc cgaccactac
      181 cagcagaaca cccctatcgg agatggacct gtgctgctgc cagacaacca ctacctgagc
      241 acccagagca agctgagcaa ggaccctaac gagaagcgcg accacatggt gctgctggaa
      301 tttgtgaccg ccgctggaat caccctggga atggacgagc tgtacaaggg cggaggaagc
      361 ggaggcggaa gcggcggggg aagcatgtct gaatatattc gggtaaccga agatgagaac
      421 gatgagccca ttgaaatacc atcggaagac gatgggacgg tgctgctctc cacggttaca
      481 gcccagtttc caggggcgtg tgggcttcgc tacaggaatc cagtgtctca gtgtatgaga
      541 ggtgtccggc tggtagaagg aattctgcat gccccagatg ctggctgggg aaatctggtg
      601 tatgttgtca actatccaaa agataacaaa agaaaaatgg atgagacaga tgcttcatca
      661 gcagtgaaag tgaaaagagc agtccagaaa acatccgatt taatagtgtt gggtctccca
      721 tggaaaacaa ccgaacagga cctgaaagag tattttagta cctttggaga agttcttatg
      781 gtgcaggtca agaaagatct taagactggt cattcaaagg ggtttggctt tgttcgtttt
      841 acggaatatg aaacacaagt gaaagtaatg tcacagcgac atatgataga tggacgatgg
      901 tgtgactgca aacttcctaa ttctaagcaa agccaagatg agcctttgag aagcagaaaa
      961 gtgtttgtgg ggcgctgtac agaggacatg actgaggatg agctgcggga gttcttctct
     1021 cagtacgggg atgtgatgga tgtcttcatc cccaagccat tcagggcctt tgcctttgtt
     1081 acatttgcag atgatcagat tgcgcagtct ctttgtggag aggacttgat cattaaagga
     1141 atcagcgttc atatatccaa tgccgaacct aagcacaata gcaatagaca gttagaaaga
     1201 agtggaagat ttggtggtaa tccaggtggc tttgggaatc agggtggatt tggtaatagc
     1261 agagggggtg gagctggttt gggaaacaat caaggtagta atatgggtgg tgggatgaac
     1321 tttggtgcgt tcagcattaa tccagccatg atggctgccg cccaggcagc actacagagc
     1381 agttggggta tgatgggcat gttagccagc cagcagaacc agtcaggccc atcgggtaat
     1441 aaccaaaacc aaggcaacat gcagagggag ccaaaccagg ccttcggttc tggaaataac
     1501 tcttatagtg gctctaattc tggtgcagca attggttggg gatcagcatc caatgcaggg
     1561 tcgggcagtg gttttaatgg aggctttggc tcaagcatgg attctaagtc ttctggctgg
     1621 ggaatgtagt ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca
     1681 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1741 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1801 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg
     1861 cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt agtgagggtt
     1921 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4081 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     4141 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4201 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     4261 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     4321 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     4381 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     4441 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     4501 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     4561 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     4621 cagtcgacca tagccaattc aatatggcgt atatggactc atgccaattc aatatggtgg
     4681 atctggacct gtgccaattc aatatggcgt atatggactc gtgccaattc aatatggtgg
     4741 atctggaccc cagccaattc aatatggcgg acttggcacc atgccaattc aatatggcgg
     4801 acttggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg
     4861 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca
     4921 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat
     4981 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg
     5041 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg
     5101 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc
     5161 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg
     5221 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac
     5281 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga
     5341 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg
     5401 caagtacatt actattggaa ggacgccagg gtacattggc agtactccca ttgacgtcaa
     5461 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg
     5521 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag
     5581 gtctatataa gcaatgctcg tttagggaac
//
