LOCUS       pFE-R.euF1Aq-PRK        6133 bp ds-DNA     circular     30-JUN-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     source          2000..3937
                     /label="source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2186..2188
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3740..3742
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2618..2623
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2615..2620
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        5980..6008
                     /label="amp prom promoter ApEinfo_revcolor=#c6c9d1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2360..2365
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2912..2917
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2246..2248
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      762..856
                     /label="lambda t0 terminator ApEinfo_revcolor=#c6c9d1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2645..2647
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     5965..5990
                     /label="pZ Strep F"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     misc_feature    2141..2143
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3518..3934
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2615..2620
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3590..3592
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3662..3667
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    1899..1917
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    3266..3271
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2765..2767
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3551..3553
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3989..4006
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2348..2353
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3581..3583
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3533..3547
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2201..2206
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2186..2188
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2369..2377
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3557..3559
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2201..2224
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3941..3967
                     /label="Spacer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2489..2491
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2192..2197
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3623..3625
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            3986..5005
                     /label="PrkA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2324..2338
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2342..2344
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2627..2641
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3518..3937
                     /label="cbbSp"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     protein_bind    1924..1942
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    2609..2611
                     /label="catalytic residue [active]"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2684..2686
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2795..2797
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3987..5005
                     /label="prkA"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        1899..1972
                     /label="PLtet prom promoter ApEinfo_revcolor=#c6c9d1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(5556..5561)
                     /label="XbaI/SpeI Scar(1)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(5939..6043)
                     /label="AmpR promoter ApEinfo_revcolor=#c6c9d1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     old_sequence    6044..6062
                     /label="pZS4Int match"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2231..2233
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2996..3013
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     RBS             1982..1993
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2375..2377
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(5191..5273)
                     /label="ampReg_pSB1A2identity"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5151..5190
                     /label="UNS2-NheI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3194..3196
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3257..3259
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2525..2527
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4928..4928
                     /label="N"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            2000..3460
                     /label="cbbLp"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3791..3793
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2537..2539
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(5315..5938)
                     /label="tetR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3215..3220
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3248..3250
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3524..3529
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2735..2752
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2780..2782
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3212..3217
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2924..2932
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3146..3154
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3404..3406
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3650..3658
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      71..690
                     /label="pBR322 origin"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3362..3364
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3398..3400
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            3518..3937
                     /label="cbbSp"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2495..2509
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2900..2908
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3278..3283
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2885..2887
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3797..3802
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2870..2872
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3296..3304
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2837..2842
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2531..2533
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(4111..4130)
                     /label="prk-R0"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     misc_feature    2396..2404
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2693..2713
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3419..3421
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2027..3430
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2000..3460
                     /label="cbbLp"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    2885..2890
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2891..2896
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2588..2596
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(86..674)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3005..3010
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      5056..5142
                     /label="rrnB T1 terminator ApEinfo_revcolor=#c6c9d1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5006..5008
                     /label="BsaI Site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2381..2392
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2819..2833
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2474..2476
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     old_sequence    5315..5938
                     /label="pZS4Int match"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2075..3424
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3140..3148
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3410..3415
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3290..3292
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2984..2986
                     /label="active site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3239..3244
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5210..5258
                     /label="Putative terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3224..3235
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(887..1681)
                     /label="kanR"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    3389..3391
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      5059..5102
                     /label="rrnB_T1 term terminator ApEinfo_revcolor=#c6c9d1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3356..3358
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2531..2545
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3233..3238
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2810..2812
                     /label="miscellaneous"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(4096..4118)
                     /label="AF81_rbc_REV"
                     /note="sequence: tgaccgtcatcaattccgtacca"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          4961..4976
                     /label="AF98-HnCB-seq-13"
                     /note="sequence: gagcgtttgacctccc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3918..3937)
                     /label="Ralstonia_plasmid_AY305378 REV"
                     /note="sequence: CAAAATTATTTCTATTAGATTCAGTAGCGGCTGCCCTCGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(55..74)
                     /label="AF69_pZE_ExpRSeq"
                     /note="sequence: cgcggcctttttacggttcc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1861..1880
                     /label="AF80_rbc_FWD"
                     /note="sequence: ataggcgtatcacgaggccc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(1978..1999)
                     /label="pFE-cbbLS-prk REV"
                     /note="sequence: ACCGATTCAGGTGCGTTCATggtacctttctcctctttaatg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1818..1843
                     /label="AF71_F1_Expr_Seq_FWD"
                     /note="sequence: GACGTCTAAGAAACCATTATTATCAT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(5104..5123)
                     /label="AF91_pFA_ExprSeqR"
                     /note="sequence: gcgttcaccgacaaacaaca"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1751..1774
                     /label="AF62"
                     /note="sequence: gaaagccatccagtttactttgca"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(788..807)
                     /label="AF60"
                     /note="sequence: gttcagaacgctcggttgcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1775..1794
                     /label="AF61"
                     /note="sequence: gggcttcccaaccttaccag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2000..2019
                     /label="Ralstonia_plasmid_AY305378 FWD"
                     /note="sequence: ttaaagaggagaaaggtaccATGAACGCACCTGAATCGGT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3938..3972
                     /label="pFE-cbbLS-prk FWD"
                     /note="sequence: CCGAGGGCAGCCGCTACTGAATCTAATAGAAATAATTTTGTTTAACTTTATAAGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
       61 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      121 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      181 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      241 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca TAgctcacgc tgtaggtatc
      301 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      361 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      421 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      481 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      541 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      601 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      661 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      721 aaaactcacg ttaagggatt ttggtcatga ctagtgcttg gattctcacc aataaaaaac
      781 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      841 tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt
      901 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
      961 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     1021 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     1081 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     1141 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     1201 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     1261 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     1321 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     1381 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     1441 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct
     1501 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     1561 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     1621 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     1681 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     1741 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     1801 ccccagctgg caattccgac gtctaagaaa ccattattat catgacatta acctataaaa
     1861 ataggcgtat cacgaggccc tttcgtcttc acctcgagtc cctatcagtg atagagattg
     1921 acatccctat cagtgataga gatactgagc acatcagcag gacgcactga ccgaattcat
     1981 taaagaggag aaaggtaccA TGAACGCACC TGAATCGGTC CAAGCCAAGC CGCGCAAGCG
     2041 CTATGACGCC GGCGTCATGA AGTACAAGGA AATGGGGTAC TGGGACGGCG ACTACGAGCC
     2101 CAAGGATACC GACCTGCTGG CGCTGTTCCG CATCACGCCG CAAGACGGCG TGGATCCGGT
     2161 CGAGGCCGCT GCCGCGGTGG CCGGCGAATC CTCCACCGCC ACCTGGACGG TGGTGTGGAC
     2221 CGACCGCCTG ACCGCCTGCG ACATGTACCG CGCCAAGGCC TACCGGGTCG ATCCGGTGCC
     2281 CAACAACCCC GAGCAGTTCT TCTGCTACGT GGCCTATGAC CTGTCGCTGT TCGAGGAAGG
     2341 CTCGATCGCC AACCTGACCG CGTCGATCAT CGGCAACGTC TTCAGCTTCA AGCCGATCAA
     2401 GGCCGCGCGC CTGGAAGACA TGCGCTTCCC GGTGGCCTAT GTGAAGACCT TCGCCGGCCC
     2461 CTCCACGGGC ATCATCGTCG AGCGCGAGCG CCTCGACAAG TTCGGCCGCC CGCTGCTGGG
     2521 CGCGACCACC AAGCCCAAGC TGGGACTGTC GGGCCGCAAC TACGGCCGCG TGGTGTATGA
     2581 GGGCCTGAAG GGCGGGCTGG ATTTCATGAA GGACGACGAG AACATCAACT CGCAGCCCTT
     2641 CATGCACTGG CGCGACCGCT TCCTGTTCGT GATGGACGCC GTCAACAAGG CTTCGGCCGC
     2701 CACCGGCGAG GTCAAGGGCA GCTACCTCAA CGTGACCGCC GGCACCATGG AAGAGATGTA
     2761 CCGGCGCGCC GAGTTCGCCA AGTCGCTGGG CTCGGTCGTC ATCATGATCG ACCTGATCGT
     2821 GGGCTGGACC TGTATCCAGT CCATGAGCAA CTGGTGCCGC CAGAACGACA TGATCCTGCA
     2881 CCTGCACCGC GCGGGCCATG GCACCTACAC CCGGCAGAAG AACCACGGCG TGTCCTTCCG
     2941 CGTGATCGCC AAATGGCTGC GCCTGGCCGG CGTCGACCAC ATGCACACCG GCACCGCGGT
     3001 GGGCAAGCTG GAAGGCGATC CGCTGACCGT GCAGGGCTAC TACAACGTCT GCCGCGATGC
     3061 GTACACCCAT ACCGACCTGA CGCGCGGGCT GTTCTTCGAC CAGGACTGGG CTTCGCTGCG
     3121 CAAGGTGATG CCGGTGGCCT CCGGCGGCAT CCATGCCGGC CAGATGCACC AGCTGATCCA
     3181 CCTCTTTGGC GACGACGTGG TGCTGCAGTT CGGCGGCGGC ACCATCGGCC ACCCGCAAGG
     3241 GATCCAGGCG GGTGCTACGG CCAACCGCGT GGCGCTGGAA GCGATGGTGC TGGCGCGCAA
     3301 CGAAGGCCGC GACATCCTCA ATGAAGGGCC GGAGATCCTG CGCGATGCCG CGCGCTGGTG
     3361 CGGACCGCTG CGGGCTGCGC TCGATACCTG GGGCGATATC AGCTTCAACT ACACGCCGAC
     3421 CGACACCTCG GACTTCGCGC CCACCGCGTC GGTGGCCTGA GCCCTGGCGC GACGCCACCA
     3481 AGGCACGACG AGCGAACCCA AAGAACGAGA GGACATCATG CGTATTACTC AAGGCACTTT
     3541 CTCTTTCCTG CCCGACCTCA CCGACGCGCA GATCACCAGC CAGCTTGAAT ACTGCCTGAA
     3601 CCAGGGCTGG GCGGTCGGCA TCGAGTACAC CGACGACCCG CATCCGCGCA ATACCTACTG
     3661 GGAGATGTTC GGCCTGCCGA TGTTCGACCT GCGCGATGCC GCCGGCATCC TGCTGGAGAT
     3721 CAACAACGCG CGCAGCACCT TCCCCAACCA CTACATCCGC GTCACGGCCT TCGATTCGAC
     3781 GCATACGGTG GAGTCGGTGG TGATGTCGTT CATCGTGAAT CGTCCGGCCG ACGAGCCCGG
     3841 CTTCCGCCTG GTGCGCCAGG AAGAGCCCGG CCGCACGATC CGCTATTCGA TCGAGAGCTA
     3901 CGCCGTGCAG GCGCGCCCCG AGGGCAGCCG CTACTGAATC TAATAGAAAT AATTTTGTTT
     3961 AACTTTATAA GCAGGACCGg cggcgatgca tcatcaccat caccacagca agccagatcg
     4021 tgttgttttg atcggcgttg ccggtgactc cggttgcggc aaatcaacct tcctaaatcg
     4081 ccttgccgac ttgtttggta cggaattgat gacggtcatc tgcttggatg actatcacag
     4141 tctcgatcgc aagggccgga aggaagcagg cgtaacggct ttggatcccc gcgccaacaa
     4201 ctttgacttg atgtatgaac aggtcaaggc gttgaagaac ggcgaaacga tcatgaagcc
     4261 gatctacaac catgaaaccg gcttgatcga tccgcccgaa aaaatcgaac ccaatcgcat
     4321 cattgtgatc gagggtctgc atccgcttta cgacgagcgc gtgcgtgaac tgctcgattt
     4381 cagcgtttac ctcgacatcg atgacgaagt caaaatcgct tggaagatcc aacgcgatat
     4441 ggcagaacgc ggccactcct acgaagatgt cctcgcctcg atcgaagcgc gccgccctga
     4501 cttcaaggcc tacattgagc cccagcgtgg ccatgcggac atcgtcatcc gcgtcatgcc
     4561 gacccagcta atccccaatg acaccgagcg caaggtgctg cgggtgcagt tgatccaacg
     4621 ggaaggccgc gatggttttg agccggctta cctgttcgac gaaggttcga ccatccagtg
     4681 gacgccctgc ggtcgtaagc tgacctgctc ctatccgggc attcgcttag cctacggccc
     4741 tgacacctac tacggtcacg aagtctcagt gcttgaggtc gacggtcagt tcgagaacct
     4801 cgaagagatg atctacgtcg agggccacct cagcaagacc gacacgcagt actacggtga
     4861 gttgacccac ctgctgctac agcacaaaga ttacccgggt tcgaacaacg gcacgggtct
     4921 gttccaagtg ctgaccggcc tgaaaatgcg ggcggcctat gagcgtttga cctcccaagc
     4981 agcacccGTC GCCGCTAGTG TCTAGATGga attcctgcag cccgggggat cccatggtac
     5041 gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     5101 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc GCTAGCAGTT
     5161 CGTAGACGGA AACAAACGCA GAATCCAAGC AAAAGGGAAT AAGGGCGACA CGGAAATGTT
     5221 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATGTCCACT
     5281 GGGTTCGTGC CTTCATCCGG ATATCCAGGA ACTGTTAAGA CCCACTTTCA CATTTAAGTT
     5341 GTTTTTCTAA TCCGCATATG ATCAATTCAA GGCCGAATAA GAAGGCTGGC TCTGCACCTT
     5401 GGTGATCAAA TAATTCGATA GCTTGTCGTA ATAATGGCGG CATACTATCA GTAGTAGGTG
     5461 TTTCCCTTTC TTCTTTAGCG ACTTGATGCT CTTGATCTTC CAATACGCAA CCTAAAGTAA
     5521 AATGCCCCAC AGCGCTGAGT GCATATAATG CATTCTCTAG TGAAAAACCT TGTTGGCATA
     5581 AAAAGGCTAA TTGATTTTCG AGAGTTTCAT ACTGTTTTTC TGTAGGCCGT GTACCTAAAT
     5641 GTACTTTTGC TCCATCGCGA TGACTTAGTA AAGCACATCT AAAACTTTTA GCGTTATTAC
     5701 GTAAAAAATC TTGCCAGCTT TCCCCTTCTA AAGGGCAAAA GTGAGTATGG TGCCTATCTA
     5761 ACATCTCAAT GGCTAAGGCG TCGAGCAAAG CCCGCTTATT TTTTACATGC CAATACAATG
     5821 TAGGCTGCTC TACACCTAGC TTCTGGGCGA GTTTACGGGT TGTTAAACCT TCGATTCCGA
     5881 CCTCATTAAG CAGCTCTAAT GCGCTGTTAA TCACTTTACT TTTATCTAAT CTAGACATac
     5941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgTTTCCCT CGACAATTCG
     6061 CGctagacct agggcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6121 cggttatcca cag
//