LOCUS       pTM_        6862 bp    DNA     circular     28-OCT-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13580761|
COMMENT     VNTDBDATE|-13580774|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTM_|
COMMENT     VNTAUTHORNAME|Nadezhda Orlova|
FEATURES             Location/Qualifiers
     misc_feature    15..83
                     /vntifkey="21"
                     /label=hTPA\SP
     misc_feature    93..122
                     /vntifkey="21"
                     /label=c-myc
     rep_origin      complement(2128..2801)
                     /vntifkey="33"
                     /label=pUC\origin
     exon            complement(2943..3803)
                     /vntifkey="61"
                     /label=bla
     promoter        complement(3798..3902)
                     /vntifkey="30"
                     /label=bla\pomoter
     RBS             184..771
                     /vntifkey="32"
                     /label=EMCV\IRES
                     /note="internal ribosomal entry site "
     exon            784..1347
                     /vntifkey="61"
                     /label=DHFR
     telomere        complement(3938..4339)
                     /vntifkey="42"
                     /label=EBVTR
     misc_feature    4349..6860
                     /vntifkey="21"
                     /label=CHO\EEF1A\UFR
     misc_feature    1349..1963
                     /vntifkey="21"
                     /label=CHO\EEF1A\DFR
     TATA_signal     5842..5847
                     /vntifkey="41"
                     /label=TATA
     intron          5905..6848
                     /vntifkey="15"
                     /label=intron1
     misc_signal     5872..5872
                     /vntifkey="87"
                     /label=PTS
     primer_bind     complement(122..138)
                     /vntifkey="28"
                     /label=SQ-MycH-R
     primer_bind     complement(831..853)
                     /vntifkey="28"
                     /label=RT-ID-R
     primer_bind     766..786
                     /vntifkey="28"
                     /label=RT-ID-F
     primer_bind     complement(293..314)
                     /vntifkey="28"
                     /label=IRESA\rev
     primer_bind     6585..6602
                     /vntifkey="28"
                     /label=SQ-5CH6-F
     misc_feature    135..167
                     /vntifkey="21"
                     /label=10\His
BASE COUNT     1618 a      1806 c      1878 g      1560 t 
ORIGIN
        1 tcgaggccgc caccatggat gcaatgaaga gagggctctg ctgtgtgctg ctgctgtgtg 
       61 gagcagtctt cgtctcggct agcgctaccg gtgaacaaaa actgatcagc gaagaggatc 
      121 tgtctgcagg cggtcatcac catcaccatc accatcacca tcactagcgg ccgcgcagtt 
      181 aacgccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 
      241 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 
      301 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 
      361 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 
      421 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 
      481 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 
      541 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 
      601 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 
      661 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 
      721 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aagatctgcc 
      781 accatggttc gaccattgaa ctgcatcgtc gccgtgtccc aaaatatggg gattggcaag 
      841 aacggagacc taccctggcc tccgctcagg aacgagttca agtacttcca aagaatgacc 
      901 acaacctctt cagtggaagg taaacagaat ctggtgatta tgggtaggaa aacctggttc 
      961 tccattcctg agaagaatcg acctctaaag gacagaatta atatagttct cagtagagaa 
     1021 ctcaaagaac caccacgagg agctcatttt cttgccaaaa gtttggatga tgccttaaga 
     1081 cttattgaac aaccggaatt ggcaagtaaa gtagacatgg tttggatagt cggaggcagt 
     1141 tctgtttacc aggaagccat gaatcaacca ggccacctca gactctttgt gacaaggatc 
     1201 atgcaggaat ttgaaagtga cacgtttttc ccagaaattg atttggggaa atataaactt 
     1261 ctcccagaat acccaggcgt cctctctgag gtccaggagg aaaaaggcat caagtataag 
     1321 tttgaagtct acgagaagaa agactaatca tattacccct aacacctgcc accccagtct 
     1381 taatcagtgg tggaagaacg gtctcagaac tgtttgtctc aattggccat ttaagtttaa 
     1441 tagtgaaaga ctggttaatg ataacaatgc atcggaaaac cttcaggagg aaaggagaat 
     1501 gttttgtgga acatttttgt gtgtgtggca gttttaagtt attagttttc aaaatcagta 
     1561 ctttttaatg gaaacaactt gaccaaaaat ctgtcacaga attttgagac ccattaaaat 
     1621 acaagtttaa tgagaagtct gtctctgtta atgctgaagt cattactaag tgcttagctt 
     1681 agcaaggtat gtggatgccc atttgtgttc caagggattg gactgttcat caggacccag 
     1741 agctgagttt caagggctca agagatggct tattacctgt gggtgtcttg aaggttctgg 
     1801 ttgggacaaa ttaggaatgt ttttggcaga catggtgact acccttcatc tgggtgagtt 
     1861 cagttgattt gtcttgagcc tttggggttt acacaagtaa atgacatcat acagttagtg 
     1921 tattgttagt gaatattaat atatgaggca ggctttgctc tagctagaca tatgtcgacg 
     1981 tccggagctc aggcgcgcca tggctcttcc gcttcctcgc tcactgactc gctgcgctcg 
     2041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
     2101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
     2161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
     2221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
     2281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     2341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 
     2401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     2461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     2521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     2581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 
     2641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     2701 aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     2761 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     2821 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     2881 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     2941 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     3001 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     3061 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     3121 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     3181 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     3241 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     3301 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     3361 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     3421 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     3481 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     3541 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     3601 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     3661 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     3721 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     3781 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     3841 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     3901 gttccgcgca catttccccg aaaagtgcca cctgcatctc atcacggtca cgcatggctg 
     3961 cgcccgcgca gcgccccccg accccccccc gatccccgga acgccagccg ccatccaccg 
     4021 cccgcagccc ccggagcccg cggaccccga ccccccgccg ctcgcccgcc gttgcgccgc 
     4081 tctctgcggg ggggctaggg ggccgcgggg ggaaggccac gccccctcca ctttttccag 
     4141 gaatgcgcgg catgacccgc cccccccatg acccgccaac acccccccga tgccccccgg 
     4201 gggtctttcc tggggggcct ttgtcagggt tgcctgtgtc acccacgggc cccttggcca 
     4261 cgcttgcctg ggtcaccccg gagccccggg tcacgtttgc cggggtcagc cctggggcgc 
     4321 ttcttcacgc tccattgcca tgcagaattc ccacatgtgg acaccggaca gtaggtcctc 
     4381 aaatgctcct tattaggttg gttcaataat atcaattgtt tgttactagg cagtgatgtt 
     4441 gtacatctgg aggagatctc ttgagcccat aatcaggtta ttaggaataa atactctaag 
     4501 gctaaaaatg tagcttagtg ataagagtgc ttgcctggtg tgctgagacc ctcggttcca 
     4561 tctccacaac cccatattcc attacaaaat accttttcac cgtccctagc attaagaaac 
     4621 aaaacaacaa agaagttttt ctttcttctg agatcctgcc cggagaggca tttaaaactg 
     4681 gccagggcca aaaaaaaaaa aaaaaaaaga aaaaaaagaa aagaaaacag gctagggccg 
     4741 gcatggtggc gcacgccttt aatcccagca cgcaggaggc agaggcaggg cggatctctg 
     4801 tgagtttgag gtcagcctgg tctacctagt gagtttcagg gcacccaggg ctaaagagac 
     4861 tgtctcaaaa acaaaacagc cacacaatca gaaccacagc aaaacgcagt tatgatcctt 
     4921 ggaactgtag gaatgacaag catttaaata ataggacgag ccatttttga gaagctctga 
     4981 tttcacaagt gtcagggatg ggctctgggc gagtaagatt gctaatgctg gcctctaaat 
     5041 gagaccacgt ggagttgatt agattctttt catgttcctc gtgctctatc aaataactgt 
     5101 acccaaatac acacacacac acacacacac aatgcgcgca cacacaaaat ccttttttag 
     5161 cttaagaagc ccagaatcag aagtaaagct aactgtggga cttaagtatt attctgaacg 
     5221 gaactcccag ggcgtgaagc gcgcttcagg cttccagaga agcagctggc gctggatgga 
     5281 atgaaccaag aggccagcac aggggcagat ccgtcgagct ctcggccacc gagctgagcc 
     5341 cttaggttct ggggctggga agggtcccta ggattgtgca cctctcccgc gggggacaag 
     5401 cagggggatg gcggggctga cgtcgggagg tggcctccac gggaagggac acccggatct 
     5461 cgacacagcc ttggcagtgg agtcaggaag ggtagggaca gattctggac gccctcttgg 
     5521 ccagctcctc accgccccac ccccgactgg agccgagagt aattcataca aaaggaggga 
     5581 tcgccttcgc ccctgggaat cccagggacc gtcgctaaat tctggccggc ctcccagccc 
     5641 ggaaccgctg tgcccgccca gcgcggcggg aggagcctgc gcctagggcg gatcgcgggt 
     5701 cggcgggaga gcacaagccc acagtccccg gcggtggggg aggggcgcgc tgagcggggg 
     5761 cccgggagcc agcgcggggc aaactgggaa agtggtgtcg tgtgctggct ccgccctctt 
     5821 cccgagggtg ggggagaacg gtataaaagt gcggtagtcg cgttggacgt tctttttcgc 
     5881 aacgggtttg ccgtcagaac gcaggtgagt ggcgggtgtg gcctccgcgg gcccgggctc 
     5941 cctcctttga gcggggtcgg accgccgtgc gggtgtcgtc ggccgggctt ctctgcgagc 
     6001 gttcccgccc tggatggcgg gctgtgcggg agggcgaggg ggggaggcct ggcggcggcc 
     6061 ccggagcctc gcctcgtgtc gggcgtgagg cctagcgtgg cttccgcccc gccgcgtgcc 
     6121 accgcggccg cgctttgctg tctgcccggc tgccctcgat tgcctgcccg cggcccgggc 
     6181 caacaaaggg agggcgtgga gctggctggt agggagcccc gtagtccgca tgtcgggcag 
     6241 ggagagcggc agcagtcggg ggggggaccg ggcccgcccg tcccgcagca catgtccgac 
     6301 gccgcctgga cgggtagcgg cctgtgtcct gataaggcgg ccgggcggtg ggttttagat 
     6361 gccgggttca ggtggccccg ggtcccggcc cggtctggcc agtaccccgt agtggcttag 
     6421 ctccgaggag ggcgaggccc gcccgcccgg caccagttgc gtgcgcggaa agatggccgc 
     6481 tcccgggccc tgtagcaagg agctcaaaat ggaggacgcg gcagcccggc ggagcggggc 
     6541 gggtgagtca cccacacaaa ggaagagggc cttgcccctc gccggccgct gcttcctgtg 
     6601 accccgtggt gtaccggccg cacttcagtc accccgggcg ctctttcgga gcaccgctgg 
     6661 cctccgctgg gggaggggat ctgtctaatg gcgttggagt ttgctcacat ttggtgggtg 
     6721 gagactgtag ccaggccagc ctggccatga aagtaattct tggaatttgc ccattttgag 
     6781 tttggagcga agctgattga caaagctgct tagccgttca aaggtattct tcgaactttt 
     6841 tttttaaggt gttgtgaaaa cc 
//
