LOCUS       pTM-RBDv2        7513 bp    DNA     circular     28-OCT-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13580773|
COMMENT     VNTDBDATE|-13580773|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTM-RBDv2|
COMMENT     VNTAUTHORNAME|Nadezhda Orlova|
FEATURES             Location/Qualifiers
     misc_feature    786..818
                     /vntifkey="21"
                     /label=10\His
     primer_bind     7236..7253
                     /vntifkey="28"
                     /label=SQ-5CH6-F
     primer_bind     complement(6268..6285)
                     /vntifkey="28"
                     /label=AD-5CHD-FseIr
     primer_bind     4995..5016
                     /vntifkey="28"
                     /label=AD-5CHD1291-EcoRIf
     primer_bind     complement(944..965)
                     /vntifkey="28"
                     /label=IRESA\rev
     primer_bind     1417..1437
                     /vntifkey="28"
                     /label=RT-ID-F
     primer_bind     complement(1482..1504)
                     /vntifkey="28"
                     /label=RT-ID-R
     primer_bind     complement(773..789)
                     /vntifkey="28"
                     /label=SQ-MycH-R
     misc_signal     6523..6523
                     /vntifkey="87"
                     /label=PTS
     intron          6556..7499
                     /vntifkey="15"
                     /label=intron1
     TATA_signal     6493..6498
                     /vntifkey="41"
                     /label=TATA
     misc_feature    2000..2614
                     /vntifkey="21"
                     /label=CHO\EEF1A\DFR
     misc_feature    5000..7511
                     /vntifkey="21"
                     /label=CHO\EEF1A\UFR
     telomere        complement(4589..4990)
                     /vntifkey="42"
                     /label=EBVTR
     exon            1435..1998
                     /vntifkey="61"
                     /label=DHFR
     RBS             835..1422
                     /vntifkey="32"
                     /label=EMCV\IRES
                     /note="internal ribosomal entry site "
     promoter        complement(4449..4553)
                     /vntifkey="30"
                     /label=bla\pomoter
     exon            complement(3594..4454)
                     /vntifkey="61"
                     /label=bla
     rep_origin      complement(2779..3452)
                     /vntifkey="33"
                     /label=pUC\origin
     misc_feature    744..773
                     /vntifkey="21"
                     /label=c-myc
     misc_feature    15..83
                     /vntifkey="21"
                     /label=hTPA\SP
     misc_feature    84..737
                     /vntifkey="21"
                     /label=RBD
BASE COUNT     1768 a      2015 c      2042 g      1688 t 
ORIGIN
        1 tcgaggccgc caccatggat gcaatgaaga gagggctctg ctgtgtgctg ctgctgtgtg 
       61 gagcagtctt cgtctcggct agcgtgcagc ccaccgaatc catcgtgcgg ttccccaata 
      121 tcaccaatct gtgccccttc ggcgaggtgt tcaatgccac cagattcgcc tctgtgtacg 
      181 cctggaaccg gaagcggatc agcaattgcg tggccgacta ctccgtgctg tacaactccg 
      241 ccagcttcag caccttcaag tgctacggcg tgtcccctac caagctgaac gacctgtgct 
      301 tcacaaacgt gtacgccgac agcttcgtga tccggggaga tgaagtgcgg cagattgccc 
      361 ctggacagac aggcaagatc gccgactaca actacaagct gcccgacgac ttcaccggct 
      421 gtgtgattgc ctggaacagc aacaacctgg actccaaagt cggcggcaac tacaattacc 
      481 tgtaccggct gttccggaag tccaatctga agcccttcga gcgggacatc tccaccgaga 
      541 tctatcaggc cggcagcacc ccttgtaacg gcgtggaagg cttcaactgc tacttcccac 
      601 tgcagtccta cggctttcag cccacaaatg gcgtgggcta tcagccctac agagtggtgg 
      661 tgctgagctt cgaactgctg catgcccctg ccacagtgtg cggccctaag aaaagcacca 
      721 atctcgtgaa gaacaaaccc ggtgaacaaa aactgatcag cgaagaggat ctgtctgcag 
      781 gcggtcatca ccatcaccat caccatcacc atcactagcg gccgcgcagt taacgccgcc 
      841 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 
      901 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 
      961 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 
     1021 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 
     1081 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 
     1141 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 
     1201 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg 
     1261 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca 
     1321 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg 
     1381 acgtggtttt cctttgaaaa acacgatgat aatatggcca caagatctgc caccatggtt 
     1441 cgaccattga actgcatcgt cgccgtgtcc caaaatatgg ggattggcaa gaacggagac 
     1501 ctaccctggc ctccgctcag gaacgagttc aagtacttcc aaagaatgac cacaacctct 
     1561 tcagtggaag gtaaacagaa tctggtgatt atgggtagga aaacctggtt ctccattcct 
     1621 gagaagaatc gacctctaaa ggacagaatt aatatagttc tcagtagaga actcaaagaa 
     1681 ccaccacgag gagctcattt tcttgccaaa agtttggatg atgccttaag acttattgaa 
     1741 caaccggaat tggcaagtaa agtagacatg gtttggatag tcggaggcag ttctgtttac 
     1801 caggaagcca tgaatcaacc aggccacctc agactctttg tgacaaggat catgcaggaa 
     1861 tttgaaagtg acacgttttt cccagaaatt gatttgggga aatataaact tctcccagaa 
     1921 tacccaggcg tcctctctga ggtccaggag gaaaaaggca tcaagtataa gtttgaagtc 
     1981 tacgagaaga aagactaatc atattacccc taacacctgc caccccagtc ttaatcagtg 
     2041 gtggaagaac ggtctcagaa ctgtttgtct caattggcca tttaagttta atagtgaaag 
     2101 actggttaat gataacaatg catcggaaaa ccttcaggag gaaaggagaa tgttttgtgg 
     2161 aacatttttg tgtgtgtggc agttttaagt tattagtttt caaaatcagt actttttaat 
     2221 ggaaacaact tgaccaaaaa tctgtcacag aattttgaga cccattaaaa tacaagttta 
     2281 atgagaagtc tgtctctgtt aatgctgaag tcattactaa gtgcttagct tagcaaggta 
     2341 tgtggatgcc catttgtgtt ccaagggatt ggactgttca tcaggaccca gagctgagtt 
     2401 tcaagggctc aagagatggc ttattacctg tgggtgtctt gaaggttctg gttgggacaa 
     2461 attaggaatg tttttggcag acatggtgac tacccttcat ctgggtgagt tcagttgatt 
     2521 tgtcttgagc ctttggggtt tacacaagta aatgacatca tacagttagt gtattgttag 
     2581 tgaatattaa tatatgaggc aggctttgct ctagctagac atatgtcgac gtccggagct 
     2641 caggcgcgcc atggctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 
     2701 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 
     2761 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 
     2821 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 
     2881 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 
     2941 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 
     3001 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 
     3061 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 
     3121 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 
     3181 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 
     3241 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 
     3301 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 
     3361 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     3421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     3481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     3541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     3601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     3661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     3721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     3781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     3841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     3901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     3961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     4021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     4081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     4141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     4201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     4261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     4321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     4381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     4441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
     4501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 
     4561 acatttcccc gaaaagtgcc acctgcatct catcacggtc acgcatggct gcgcccgcgc 
     4621 agcgcccccc gacccccccc cgatccccgg aacgccagcc gccatccacc gcccgcagcc 
     4681 cccggagccc gcggaccccg accccccgcc gctcgcccgc cgttgcgccg ctctctgcgg 
     4741 gggggctagg gggccgcggg gggaaggcca cgccccctcc actttttcca ggaatgcgcg 
     4801 gcatgacccg ccccccccat gacccgccaa cacccccccg atgccccccg ggggtctttc 
     4861 ctggggggcc tttgtcaggg ttgcctgtgt cacccacggg ccccttggcc acgcttgcct 
     4921 gggtcacccc ggagccccgg gtcacgtttg ccggggtcag ccctggggcg cttcttcacg 
     4981 ctccattgcc atgcagaatt cccacatgtg gacaccggac agtaggtcct caaatgctcc 
     5041 ttattaggtt ggttcaataa tatcaattgt ttgttactag gcagtgatgt tgtacatctg 
     5101 gaggagatct cttgagccca taatcaggtt attaggaata aatactctaa ggctaaaaat 
     5161 gtagcttagt gataagagtg cttgcctggt gtgctgagac cctcggttcc atctccacaa 
     5221 ccccatattc cattacaaaa taccttttca ccgtccctag cattaagaaa caaaacaaca 
     5281 aagaagtttt tctttcttct gagatcctgc ccggagaggc atttaaaact ggccagggcc 
     5341 aaaaaaaaaa aaaaaaaaag aaaaaaaaga aaagaaaaca ggctagggcc ggcatggtgg 
     5401 cgcacgcctt taatcccagc acgcaggagg cagaggcagg gcggatctct gtgagtttga 
     5461 ggtcagcctg gtctacctag tgagtttcag ggcacccagg gctaaagaga ctgtctcaaa 
     5521 aacaaaacag ccacacaatc agaaccacag caaaacgcag ttatgatcct tggaactgta 
     5581 ggaatgacaa gcatttaaat aataggacga gccatttttg agaagctctg atttcacaag 
     5641 tgtcagggat gggctctggg cgagtaagat tgctaatgct ggcctctaaa tgagaccacg 
     5701 tggagttgat tagattcttt tcatgttcct cgtgctctat caaataactg tacccaaata 
     5761 cacacacaca cacacacaca caatgcgcgc acacacaaaa tcctttttta gcttaagaag 
     5821 cccagaatca gaagtaaagc taactgtggg acttaagtat tattctgaac ggaactccca 
     5881 gggcgtgaag cgcgcttcag gcttccagag aagcagctgg cgctggatgg aatgaaccaa 
     5941 gaggccagca caggggcaga tccgtcgagc tctcggccac cgagctgagc ccttaggttc 
     6001 tggggctggg aagggtccct aggattgtgc acctctcccg cgggggacaa gcagggggat 
     6061 ggcggggctg acgtcgggag gtggcctcca cgggaaggga cacccggatc tcgacacagc 
     6121 cttggcagtg gagtcaggaa gggtagggac agattctgga cgccctcttg gccagctcct 
     6181 caccgcccca cccccgactg gagccgagag taattcatac aaaaggaggg atcgccttcg 
     6241 cccctgggaa tcccagggac cgtcgctaaa ttctggccgg cctcccagcc cggaaccgct 
     6301 gtgcccgccc agcgcggcgg gaggagcctg cgcctagggc ggatcgcggg tcggcgggag 
     6361 agcacaagcc cacagtcccc ggcggtgggg gaggggcgcg ctgagcgggg gcccgggagc 
     6421 cagcgcgggg caaactggga aagtggtgtc gtgtgctggc tccgccctct tcccgagggt 
     6481 gggggagaac ggtataaaag tgcggtagtc gcgttggacg ttctttttcg caacgggttt 
     6541 gccgtcagaa cgcaggtgag tggcgggtgt ggcctccgcg ggcccgggct ccctcctttg 
     6601 agcggggtcg gaccgccgtg cgggtgtcgt cggccgggct tctctgcgag cgttcccgcc 
     6661 ctggatggcg ggctgtgcgg gagggcgagg gggggaggcc tggcggcggc cccggagcct 
     6721 cgcctcgtgt cgggcgtgag gcctagcgtg gcttccgccc cgccgcgtgc caccgcggcc 
     6781 gcgctttgct gtctgcccgg ctgccctcga ttgcctgccc gcggcccggg ccaacaaagg 
     6841 gagggcgtgg agctggctgg tagggagccc cgtagtccgc atgtcgggca gggagagcgg 
     6901 cagcagtcgg gggggggacc gggcccgccc gtcccgcagc acatgtccga cgccgcctgg 
     6961 acgggtagcg gcctgtgtcc tgataaggcg gccgggcggt gggttttaga tgccgggttc 
     7021 aggtggcccc gggtcccggc ccggtctggc cagtaccccg tagtggctta gctccgagga 
     7081 gggcgaggcc cgcccgcccg gcaccagttg cgtgcgcgga aagatggccg ctcccgggcc 
     7141 ctgtagcaag gagctcaaaa tggaggacgc ggcagcccgg cggagcgggg cgggtgagtc 
     7201 acccacacaa aggaagaggg ccttgcccct cgccggccgc tgcttcctgt gaccccgtgg 
     7261 tgtaccggcc gcacttcagt caccccgggc gctctttcgg agcaccgctg gcctccgctg 
     7321 ggggagggga tctgtctaat ggcgttggag tttgctcaca tttggtgggt ggagactgta 
     7381 gccaggccag cctggccatg aaagtaattc ttggaatttg cccattttga gtttggagcg 
     7441 aagctgattg acaaagctgc ttagccgttc aaaggtattc ttcgaacttt ttttttaagg 
     7501 tgttgtgaaa acc 
//
