LOCUS       pTF-ACE2        9005 bp    DNA     circular     28-OCT-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13580870|
COMMENT     VNTDBDATE|-13580871|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTF-ACE2|
COMMENT     VNTAUTHORNAME|Nadezhda Orlova|
FEATURES             Location/Qualifiers
     primer_bind     1442..1459
                     /vntifkey="28"
                     /label=SQ-ACE1512-F
     primer_bind     1100..1119
                     /vntifkey="28"
                     /label=SQ-ACE1170-F
     primer_bind     522..538
                     /vntifkey="28"
                     /label=SQ-ACE592-F
     primer_bind     complement(790..805)
                     /vntifkey="28"
                     /label=SQ-ACE875-R
     misc_feature    15..2234
                     /vntifkey="21"
                     /label=ACE2
     misc_feature    6..14
                     /vntifkey="21"
                     /label=Kozak
     misc_feature    15..65
                     /vntifkey="21"
                     /label=hTPA\SP
     misc_signal     8015..8015
                     /vntifkey="87"
                     /label=PTS
     intron          8048..8991
                     /vntifkey="15"
                     /label=intron1
     TATA_signal     7985..7990
                     /vntifkey="41"
                     /label=TATA
     misc_feature    3492..4106
                     /vntifkey="21"
                     /label=CHO\EEF1A\DFR
     misc_feature    6492..9003
                     /vntifkey="21"
                     /label=CHO\EEF1A\UFR
     telomere        complement(6081..6482)
                     /vntifkey="42"
                     /label=EBVTR
     exon            2927..3490
                     /vntifkey="61"
                     /label=DHFR
     RBS             2327..2914
                     /vntifkey="32"
                     /label=EMCV\IRES
                     /note="internal ribosomal entry site "
     promoter        complement(5941..6045)
                     /vntifkey="30"
                     /label=bla\pomoter
     exon            complement(5086..5946)
                     /vntifkey="61"
                     /label=bla
     rep_origin      complement(4271..4944)
                     /vntifkey="33"
                     /label=pUC\origin
     primer_bind     8728..8745
                     /vntifkey="28"
                     /label=SQ-5CH6-F
     primer_bind     complement(7760..7777)
                     /vntifkey="28"
                     /label=AD-5CHD-FseIr
     primer_bind     6487..6508
                     /vntifkey="28"
                     /label=AD-5CHD1291-EcoRIf
     primer_bind     complement(2436..2457)
                     /vntifkey="28"
                     /label=IRESA\rev
     primer_bind     2909..2929
                     /vntifkey="28"
                     /label=RT-ID-F
     primer_bind     complement(2974..2996)
                     /vntifkey="28"
                     /label=RT-ID-R
     primer_bind     2772..2791
                     /vntifkey="28"
                     /label=AD-KIuniv-F
     primer_bind     complement(3596..3615)
                     /vntifkey="28"
                     /label=AD-KIpDpN-R
     misc_feature    2274..2303
                     /vntifkey="21"
                     /label=10xHis
     misc_feature    2241..2264
                     /vntifkey="21"
                     /label=FLAG
BASE COUNT     2287 a      2243 c      2356 g      2119 t 
ORIGIN
        1 tcgaggccgc caccatgtca agctcttcct ggctccttct cagccttgtt gctgtaactg 
       61 ctgctcagtc caccattgag gaacaggcca agacattttt ggacaagttt aaccacgaag 
      121 ccgaagacct gttctatcaa agttcacttg cttcttggaa ttataacacc aatattactg 
      181 aagagaatgt ccaaaacatg aataatgctg gggacaaatg gtctgccttt ttaaaggaac 
      241 agtccacact tgcccaaatg tatccactac aagaaattca gaatctcaca gtcaagcttc 
      301 agctgcaggc tcttcagcaa aatgggtctt cagtgctctc agaagacaag agcaaacggt 
      361 tgaacacaat tctaaataca atgagcacca tctacagtac tggaaaagtt tgtaacccag 
      421 ataatccaca agaatgctta ttacttgaac caggtttgaa tgaaataatg gcaaacagtt 
      481 tagactacaa tgagaggctc tgggcttggg aaagctggag atctgaggtc ggcaagcagc 
      541 tgaggccatt atatgaagag tatgtggtct tgaaaaatga gatggcaaga gcaaatcatt 
      601 atgaggacta tggggattat tggagaggag actatgaagt aaatggggta gatggctatg 
      661 actacagccg cggccagttg attgaagatg tggaacatac ctttgaagag attaaaccat 
      721 tatatgaaca tcttcatgcc tatgtgaggg caaagttgat gaatgcctat ccttcctata 
      781 tcagtccaat tggatgcctc cctgctcatt tgcttggtga tatgtggggt agattttgga 
      841 caaatctgta ctctttgaca gttccctttg gacagaaacc aaacatagat gttactgatg 
      901 caatggtgga ccaggcctgg gatgcacaga gaatattcaa ggaggccgag aagttctttg 
      961 tatctgttgg tcttcctaat atgactcaag gattctggga aaattccatg ctaacggacc 
     1021 caggaaatgt tcagaaagca gtctgccatc ccacagcttg ggacctgggg aagggcgact 
     1081 tcaggatcct tatgtgcaca aaggtgacaa tggacgactt cctgacagct catcatgaga 
     1141 tggggcatat ccagtatgat atggcatatg ctgcacaacc ttttctgcta agaaatggag 
     1201 ctaatgaagg attccatgaa gctgttgggg aaatcatgtc actttctgca gccacaccta 
     1261 agcatttaaa atccattggt cttctgtcac ccgattttca agaagacaat gaaacagaaa 
     1321 taaacttcct gctcaaacaa gcactcacga ttgttgggac tctgccattt acttacatgt 
     1381 tagagaagtg gaggtggatg gtctttaaag gggaaattcc caaagaccag tggatgaaaa 
     1441 agtggtggga gatgaagcga gagatagttg gggtggtgga acctgtgccc catgatgaaa 
     1501 catactgtga ccccgcatct ctgttccatg tttctaatga ttactcattc attcgatatt 
     1561 acacaaggac cctttaccaa ttccagtttc aagaagcact ttgtcaagca gctaaacatg 
     1621 aaggccctct gcacaaatgt gacatctcaa actctacaga agctggacag aaactgttca 
     1681 atatgctgag gcttggaaaa tcagaaccct ggaccctagc attggaaaat gttgtaggag 
     1741 caaagaacat gaatgtaagg ccactgctca actactttga gcccttattt acctggctga 
     1801 aagaccagaa caagaattct tttgtgggat ggagtaccga ctggagtcca tatgcagacc 
     1861 aaagcatcaa agtgaggata agcctaaaat cagctcttgg agataaagca tatgaatgga 
     1921 acgacaatga aatgtacctg ttccgatcat ctgttgcata tgctatgagg cagtactttt 
     1981 taaaagtaaa aaatcagatg attctttttg gggaggagga tgtgcgagtg gctaatttga 
     2041 aaccaagaat ctcctttaat ttctttgtca ctgcacctaa aaatgtgtct gatatcattc 
     2101 ctagaactga agttgaaaag gccatcagga tgtcccggag ccgtatcaat gatgctttcc 
     2161 gtctgaatga caacagccta gagtttctgg ggatacagcc aacacttgga cctcctaacc 
     2221 agccccctgt ttccgctagc gattacaagg atgacgacga taagggagga tcccatcacc 
     2281 atcaccatca ccatcatcac cactaactag cggccgcgca gttaacgccg cccctctccc 
     2341 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 
     2401 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 
     2461 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 
     2521 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 
     2581 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 
     2641 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 
     2701 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag 
     2761 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt 
     2821 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 
     2881 ttcctttgaa aaacacgatg ataatatggc cacaagatct gccaccatgg ttcgaccatt 
     2941 gaactgcatc gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg 
     3001 gcctccgctc aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga 
     3061 aggtaaacag aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa 
     3121 tcgacctcta aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg 
     3181 aggagctcat tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga 
     3241 attggcaagt aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc 
     3301 catgaatcaa ccaggccacc tcagactctt tgtgacaagg atcatgcagg aatttgaaag 
     3361 tgacacgttt ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg 
     3421 cgtcctctct gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa 
     3481 gaaagactaa tcatattacc cctaacacct gccaccccag tcttaatcag tggtggaaga 
     3541 acggtctcag aactgtttgt ctcaattggc catttaagtt taatagtgaa agactggtta 
     3601 atgataacaa tgcatcggaa aaccttcagg aggaaaggag aatgttttgt ggaacatttt 
     3661 tgtgtgtgtg gcagttttaa gttattagtt ttcaaaatca gtacttttta atggaaacaa 
     3721 cttgaccaaa aatctgtcac agaattttga gacccattaa aatacaagtt taatgagaag 
     3781 tctgtctctg ttaatgctga agtcattact aagtgcttag cttagcaagg tatgtggatg 
     3841 cccatttgtg ttccaaggga ttggactgtt catcaggacc cagagctgag tttcaagggc 
     3901 tcaagagatg gcttattacc tgtgggtgtc ttgaaggttc tggttgggac aaattaggaa 
     3961 tgtttttggc agacatggtg actacccttc atctgggtga gttcagttga tttgtcttga 
     4021 gcctttgggg tttacacaag taaatgacat catacagtta gtgtattgtt agtgaatatt 
     4081 aatatatgag gcaggctttg ctctagctag acatatgtcg acgtccggag ctcaggcgcg 
     4141 ccatggctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     4201 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     4261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     4321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     4381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     4441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     4501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     4561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     4621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     4681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     4741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     4801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     4861 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     4921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     4981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
     5041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     5101 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     5161 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     5221 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     5281 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     5341 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     5401 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     5461 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     5521 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     5581 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     5641 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     5701 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     5761 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     5821 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     5881 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     5941 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     6001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     6061 ccgaaaagtg ccacctgcat ctcatcacgg tcacgcatgg ctgcgcccgc gcagcgcccc 
     6121 ccgacccccc cccgatcccc ggaacgccag ccgccatcca ccgcccgcag cccccggagc 
     6181 ccgcggaccc cgaccccccg ccgctcgccc gccgttgcgc cgctctctgc gggggggcta 
     6241 gggggccgcg gggggaaggc cacgccccct ccactttttc caggaatgcg cggcatgacc 
     6301 cgcccccccc atgacccgcc aacacccccc cgatgccccc cgggggtctt tcctgggggg 
     6361 cctttgtcag ggttgcctgt gtcacccacg ggccccttgg ccacgcttgc ctgggtcacc 
     6421 ccggagcccc gggtcacgtt tgccggggtc agccctgggg cgcttcttca cgctccattg 
     6481 ccatgcagaa ttcccacatg tggacaccgg acagtaggtc ctcaaatgct ccttattagg 
     6541 ttggttcaat aatatcaatt gtttgttact aggcagtgat gttgtacatc tggaggagat 
     6601 ctcttgagcc cataatcagg ttattaggaa taaatactct aaggctaaaa atgtagctta 
     6661 gtgataagag tgcttgcctg gtgtgctgag accctcggtt ccatctccac aaccccatat 
     6721 tccattacaa aatacctttt caccgtccct agcattaaga aacaaaacaa caaagaagtt 
     6781 tttctttctt ctgagatcct gcccggagag gcatttaaaa ctggccaggg ccaaaaaaaa 
     6841 aaaaaaaaaa agaaaaaaaa gaaaagaaaa caggctaggg ccggcatggt ggcgcacgcc 
     6901 tttaatccca gcacgcagga ggcagaggca gggcggatct ctgtgagttt gaggtcagcc 
     6961 tggtctacct agtgagtttc agggcaccca gggctaaaga gactgtctca aaaacaaaac 
     7021 agccacacaa tcagaaccac agcaaaacgc agttatgatc cttggaactg taggaatgac 
     7081 aagcatttaa ataataggac gagccatttt tgagaagctc tgatttcaca agtgtcaggg 
     7141 atgggctctg ggcgagtaag attgctaatg ctggcctcta aatgagacca cgtggagttg 
     7201 attagattct tttcatgttc ctcgtgctct atcaaataac tgtacccaaa tacacacaca 
     7261 cacacacaca cacaatgcgc gcacacacaa aatccttttt tagcttaaga agcccagaat 
     7321 cagaagtaaa gctaactgtg ggacttaagt attattctga acggaactcc cagggcgtga 
     7381 agcgcgcttc aggcttccag agaagcagct ggcgctggat ggaatgaacc aagaggccag 
     7441 cacaggggca gatccgtcga gctctcggcc accgagctga gcccttaggt tctggggctg 
     7501 ggaagggtcc ctaggattgt gcacctctcc cgcgggggac aagcaggggg atggcggggc 
     7561 tgacgtcggg aggtggcctc cacgggaagg gacacccgga tctcgacaca gccttggcag 
     7621 tggagtcagg aagggtaggg acagattctg gacgccctct tggccagctc ctcaccgccc 
     7681 cacccccgac tggagccgag agtaattcat acaaaaggag ggatcgcctt cgcccctggg 
     7741 aatcccaggg accgtcgcta aattctggcc ggcctcccag cccggaaccg ctgtgcccgc 
     7801 ccagcgcggc gggaggagcc tgcgcctagg gcggatcgcg ggtcggcggg agagcacaag 
     7861 cccacagtcc ccggcggtgg gggaggggcg cgctgagcgg gggcccggga gccagcgcgg 
     7921 ggcaaactgg gaaagtggtg tcgtgtgctg gctccgccct cttcccgagg gtgggggaga 
     7981 acggtataaa agtgcggtag tcgcgttgga cgttcttttt cgcaacgggt ttgccgtcag 
     8041 aacgcaggtg agtggcgggt gtggcctccg cgggcccggg ctccctcctt tgagcggggt 
     8101 cggaccgccg tgcgggtgtc gtcggccggg cttctctgcg agcgttcccg ccctggatgg 
     8161 cgggctgtgc gggagggcga gggggggagg cctggcggcg gccccggagc ctcgcctcgt 
     8221 gtcgggcgtg aggcctagcg tggcttccgc cccgccgcgt gccaccgcgg ccgcgctttg 
     8281 ctgtctgccc ggctgccctc gattgcctgc ccgcggcccg ggccaacaaa gggagggcgt 
     8341 ggagctggct ggtagggagc cccgtagtcc gcatgtcggg cagggagagc ggcagcagtc 
     8401 ggggggggga ccgggcccgc ccgtcccgca gcacatgtcc gacgccgcct ggacgggtag 
     8461 cggcctgtgt cctgataagg cggccgggcg gtgggtttta gatgccgggt tcaggtggcc 
     8521 ccgggtcccg gcccggtctg gccagtaccc cgtagtggct tagctccgag gagggcgagg 
     8581 cccgcccgcc cggcaccagt tgcgtgcgcg gaaagatggc cgctcccggg ccctgtagca 
     8641 aggagctcaa aatggaggac gcggcagccc ggcggagcgg ggcgggtgag tcacccacac 
     8701 aaaggaagag ggccttgccc ctcgccggcc gctgcttcct gtgaccccgt ggtgtaccgg 
     8761 ccgcacttca gtcaccccgg gcgctctttc ggagcaccgc tggcctccgc tgggggaggg 
     8821 gatctgtcta atggcgttgg agtttgctca catttggtgg gtggagactg tagccaggcc 
     8881 agcctggcca tgaaagtaat tcttggaatt tgcccatttt gagtttggag cgaagctgat 
     8941 tgacaaagct gcttagccgt tcaaaggtat tcttcgaact ttttttttaa ggtgttgtga 
     9001 aaacc 
//
