LOCUS       pHYP-NPC-10H        6345 bp    DNA     circular      7-APR-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13288410|
COMMENT     VNTDBDATE|-13288411|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pHYP-NPC-10H|
COMMENT     VNTAUTHORNAME|Nadezhda Orlova|
FEATURES             Location/Qualifiers
     precursor_RNA   2169..2393
                     /vntifkey="55"
                     /label=Sok
     exon            complement(1967..2125)
                     /vntifkey="61"
                     /label=Hok
     misc_feature    complement(1841..2436)
                     /vntifkey="21"
                     /label=HS
     terminator      1399..1445
                     /vntifkey="43"
                     /label=T7_term
     promoter        6260..6276
                     /vntifkey="30"
                     /label=T7_prom
     gene            5965..6228
                     /gene="tet(564-300)"
                     /vntifkey="60"
     exon            complement(4791..5873)
                     /vntifkey="61"
                     /label=lacI
     gene            4457..4732
                     /gene="tet(852-576)"
                     /vntifkey="60"
     exon            complement(2626..3441)
                     /gene="KanR2"
                     /vntifkey="61"
                     /label=KanR2
                     /note="Orf frame 2"
     gene            complement(2626..3441)
                     /gene="KanR2"
                     /vntifkey="60"
     rep_origin      3547..4166
                     /vntifkey="33"
                     /label=pBR322\ori
     exon            2..1288
                     /vntifkey="61"
                     /label=NPC
BASE COUNT     1587 a      1723 c      1612 g      1423 t 
ORIGIN
        1 catgtcagac aatggtcctc agaaccagcg taatgctcct aggatcactt tcggcggtcc 
       61 aagtgattcg accggcagca atcaaaatgg tgagcgatcc ggggcgcgta gcaagcagcg 
      121 ccgccctcaa ggtctcccga acaatacagc gtcttggttc actgcgctga cgcagcatgg 
      181 aaaggaggat cttaagtttc cgcgtgggca gggcgtgccg atcaacacaa acagttcccc 
      241 agatgatcag ataggctact accggcgcgc gacgcgacga atacgcggcg gagacggcaa 
      301 gatgaaggac ctgagtccgc ggtggtactt ttactatctg ggcacagggc cagaagcggg 
      361 cctaccgtac ggcgccaaca aagatggcat catatgggta gcgacagaag gggccctgaa 
      421 cacccctaag gatcatattg gcacaaggaa ccctgccaac aatgctgcca tcgtgcttca 
      481 actcccgcaa ggtactactt tgccgaaggg cttctacgca gagggttcgc gcggtggctc 
      541 acaggcgagt tcacgcagta gtagtcggtc ccggaattca tcccgaaact cgacacccgg 
      601 ctctagccgt ggtactagcc cagctagaat ggcaggcaat ggaggggatg ccgcgctagc 
      661 cttgctgctc ctcgatagac tcaaccagct cgaaagcaag atgtcaggaa agggccagca 
      721 gcagcaagga cagactgtga cgaagaaaag cgcggctgaa gcgagcaaga agccaagaca 
      781 aaagcgcacc gcaacaaagg cgtacaatgt tacacaggct tttggaagac gcggtccgga 
      841 acaaacgcag ggtaattttg gagaccaaga gttaattagg cagggtacgg actacaagca 
      901 ttggcctcag atcgcccaat ttgccccgtc ggcgtccgcg ttctttggga tgtcgcgcat 
      961 tggaatggaa gttaccccga gcggcacctg gttaacatac accggtgcaa ttaagctaga 
     1021 tgataaagat ccaaacttca aagaccaagt aattctactc aacaagcaca tcgatgccta 
     1081 caagactttt cctccaacag agcctaagaa ggacaagaag aagaaagctg acgaaactca 
     1141 ggcactaccc caacgtcaaa agaagcaaca gacagtcaca ctccttcccg ccgcggattt 
     1201 agatgatttc tcgaaacagc tgcaacagtc aatgtctagc gcggatagta cacaggcgca 
     1261 tcaccatcat caccaccatc accatcactg ataagcttgc ggccgcactc gagcaccacc 
     1321 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg 
     1381 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt 
     1441 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc 
     1501 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     1561 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     1621 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
     1681 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     1741 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     1801 aacaacactc aaccctatct cggtctattc ttttgattta gtgcatgcac aacatcagca 
     1861 aggagaaagg ggctaccggc gaaccagcag cccctttata aaggcgcttc agtagtcaga 
     1921 ccagcatcag tcctgaaaag gcgggcctgc gcccgcctcc aggttgctac ttaccggatt 
     1981 cgtaagccat gaaagccgcc acctccctgt gtccgtctct gtaacgaatc tcgcacagcg 
     2041 attttcgtgt cagataagtg aatatcaaca gtgtgagaca cacgatcaac acacaccaga 
     2101 caagggaact tcgtggtagt ttcatggcct tcttctcctt gcgcaaagcg cggtaagagg 
     2161 ctatcctgat gtggactaga catagggatg cctcgtggtg gttaatgaaa attaacttac 
     2221 tacggggcta tcttctttct gccacacaac acggcaacaa accaccttca cgtcatgagg 
     2281 cagaaagcct caagcgccgg gcacatcata gcccatatac ctgcacgctg accacactca 
     2341 ctttccctga aaataatccg ctcattcaga ccgttcacgg gaaatccgtg tgattgttgc 
     2401 cgcatcacgc tgcctcccgg agtttgttgg atccactaag ggattttgcc gatttcggcc 
     2461 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 
     2521 acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 
     2581 tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc 
     2641 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa 
     2701 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc 
     2761 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc 
     2821 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa 
     2881 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc 
     2941 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg 
     3001 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag 
     3061 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg 
     3121 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat 
     3181 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc 
     3241 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc 
     3301 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca 
     3361 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt 
     3421 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt 
     3481 tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     3541 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     3601 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     3661 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 
     3721 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     3781 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     3841 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     3901 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     3961 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     4021 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     4081 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     4141 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     4201 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     4261 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     4321 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 
     4381 accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta 
     4441 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg 
     4501 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca 
     4561 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg 
     4621 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag 
     4681 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc 
     4741 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg 
     4801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     4861 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac 
     4921 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt 
     4981 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg 
     5041 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg 
     5101 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga 
     5161 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg 
     5221 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc 
     5281 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga 
     5341 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata 
     5401 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca 
     5461 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg 
     5521 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc 
     5581 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt 
     5641 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg 
     5701 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc 
     5761 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc 
     5821 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc 
     5881 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat 
     5941 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc 
     6001 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat 
     6061 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct 
     6121 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc 
     6181 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga 
     6241 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat 
     6301 tcccctctag aaataatttt gtttaacttt aagaaggaga tatac 
//
