LOCUS       Exported                8305 bp ds-DNA     circular SYN 28-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 28, 2020 from SnapGene 5.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    411..1211
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    complement(1343..1798)
                     /locus_tag="M13 Origin"
                     /label=M13 Origin
     misc_feature    complement(1475..1781)
                     /locus_tag="F1 ori"
                     /label=F1 ori
     misc_feature    complement(1803..1871)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     misc_feature    1942..1959
                     /locus_tag="M13 Fwd"
                     /label=M13 Fwd
     misc_feature    1958..1985
                     /locus_tag="T7"
                     /label=T7
     misc_feature    1999..2402
                     /locus_tag="Tef1p(2)"
                     /label=Tef1p
     misc_feature    2409..3134
                     /locus_tag="ymCherry"
                     /label=ymCherry
     misc_feature    3143..3404
                     /locus_tag="CYC1t"
                     /label=CYC1t
     misc_feature    3945..3964
                     /locus_tag="Tef1-F2"
                     /label=Tef1-F2
     misc_feature    3965..4366
                     /label=Tef1p
     misc_feature    4224..4243
                     /locus_tag="TEF1-F"
                     /label=TEF1-F
     misc_feature    4421..5137
                     /label=EGFP
     misc_feature    5094..5119
                     /locus_tag="GFP_Seq-F"
                     /label=GFP_Seq-F
     misc_feature    5146..5268
                     /locus_tag="Aptamer invII_of_4-14m"
                     /label=2_6m_RNA-device
     misc_feature    5274..5513
                     /label=ADH1t
     misc_feature    complement(5281..5309)
                     /locus_tag="ADHt_seq-R"
                     /label=ADHt_seq-R
     misc_feature    complement(5529..5548)
                     /locus_tag="t3"
                     /label=t3
     misc_feature    complement(5531..5549)
                     /locus_tag="T3 promoter"
                     /label=T3 promoter
     misc_feature    complement(5566..5586)
                     /locus_tag="M13-R"
                     /label=M13-R
     misc_feature    complement(5592..5614)
                     /locus_tag="LacO"
                     /label=LacO
     misc_feature    complement(5954..6636)
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(6734..7393)
                     /locus_tag="AmpR"
                     /label=AmpR
     misc_feature    7723..8241
                     /locus_tag="CEN6_ARSH4"
                     /label=CEN6_ARSH4
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     1981 tagggcgaat tggagctcat agcttcaaaa tgtttctact ccttttttac tcttccagat
     2041 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
     2101 ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
     2161 aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg
     2221 tttctttttc ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga
     2281 tatttaagtt aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta
     2341 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
     2401 tgccgcggga aataatgtct atggttagta aaggagaaga aaataacatg gcaatcatta
     2461 aggagttcat gagattcaaa gttcacatgg aaggttctgt aaatggacat gaatttgaaa
     2521 tagaaggtga aggagaagga aggccttatg aaggaaccca aaccgcgaag ctaaaagtta
     2581 ctaagggtgg cccattacca tttgcatggg atatccttag ccctcaattc atgtatgggt
     2641 caaaggctta tgtcaagcac cccgccgaca ttccagacta tctaaagtta tcttttcccg
     2701 aagggtttaa gtgggagcgt gtgatgaact tcgaagacgg tggcgtggta acagtgactc
     2761 aggattcgtc cctgcaagat ggtgaattta tctacaaagt caaattaaga ggaactaact
     2821 ttccatctga cggcccggtt atgcaaaaaa agacaatggg ctgggaggcc tcctcagaac
     2881 gaatgtaccc tgaagatggt gccttgaagg gtgagattaa acaaagattg aaattgaaag
     2941 atggtggaca ttatgacgct gaggttaaaa cgacatacaa agctaagaaa cctgtccagc
     3001 tcccaggtgc ttacaatgta aatataaaac ttgatattac atcacataat gaagattata
     3061 cgatagttga acaatacgaa agggctgagg ggagacatag tactggtggc atggatgaac
     3121 tatacaaata ataagcggcc gcgagggccg catcatgtaa ttagttatgt cacgcttaca
     3181 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     3241 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     3301 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
     3361 gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggcctctaga cgccttgagc
     3421 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     3481 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     3541 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     3601 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     3661 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     3721 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     3781 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     3841 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     3901 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     3961 atcgatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc tcggactccg
     4021 cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaatttcc cctctttctt
     4081 cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa gagaccgcct
     4141 cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc tttttcttga
     4201 aaattttttt ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa
     4261 cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
     4321 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttgggat cccccgggct
     4381 gcaggaattc gatatcaagc ttggagatct aaaagaaata atgtctaaag gtgaagaatt
     4441 attcactggt gttgtcccaa ttttggttga attagatggt gatgttaatg gtcacaaatt
     4501 ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt aaattgacct taaaatttat
     4561 ttgtactact ggtaaattgc cagttccatg gccaacctta gtcactactt tcggttatgg
     4621 tgttcaatgt tttgcgagat acccagatca tatgaaacaa catgactttt tcaagtctgc
     4681 catgccagaa ggttatgttc aagaaagaac tatttttttc aaagatgacg gtaactacaa
     4741 gaccagagct gaagtcaagt ttgaaggtga taccttagtt aatagaatcg aattaaaagg
     4801 tattgatttt aaagaagatg gtaacatttt aggtcacaaa ttggaataca actataactc
     4861 tcacaatgtt tacatcatgg ctgacaaaca aaagaatggt atcaaagtta acttcaaaat
     4921 tagacacaac attgaagatg gttctgttca attagctgac cattatcaac aaaatactcc
     4981 aattggtgat ggtccagtct tgttaccaga caaccattac ttatccactc aatctgcctt
     5041 atccaaagat ccaaacgaaa agagagacca catggtcttg ttagaatttg ttactgctgc
     5101 tggtattatc catggtatgg atgaattgta caaataaagc ctaggaaaca aacaaagctg
     5161 tcaccggaat aggagtccgg tctgatgagt cctgttgctg aggatccgac ggttcctcat
     5221 gtgaactgct tggacgaaac agcaaaaaga aaaataaaaa ctcgagcctc gagagggcgc
     5281 gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata
     5341 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
     5401 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta tgaggtcgct
     5461 cttattgacc acacctctac cggcagatcc gctagggata acagggtaat ataggtacca
     5521 gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg tcatagctgt
     5581 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cataggagcc ggaagcataa
     5641 agtgtaaagc ctggggtgcc taatgagtga ggtaactcac attaattgcg ttgcgctcac
     5701 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5761 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctcggcccc cctgacgagc
     6001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6061 aggcgttccc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     6181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6421 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6781 gttcatccat agttgcctga ctgcccgtcg tgtagataac tacgatacgg gagggcttac
     6841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7141 gaaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7681 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtccttt tcatcacgtg
     7741 ctataaaaat aattataatt taaatttttt aatataaata tataaattaa aaatagaaag
     7801 taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg
     7861 gggatcgcca acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg
     7921 aattgtttca tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt
     7981 acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata
     8041 tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc
     8101 cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa
     8161 ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag
     8221 ttacaggcaa gcgatccgtc ctaagaaacc attattatca tgacattaac ctataaaaat
     8281 aggcgtatca cgaggccctt tcgtc
//
