LOCUS       Exported                5021 bp ds-DNA     circular SYN 16-OCT-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 5, 2020 from SnapGene 5.0.4
            https://www.snapgene.com
COMMENT     Description Vector for fusing EGFP to the C-terminus of a partner 
            protein.�For other reading frames, use pEGFP-N2 or pEGFP-N3.

            Comments
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #400040"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #8080ff; direction: RIGHT"
     primer_bind     519..539
                     /label=CMV Forward Primer
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    591..621
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
                     /note="color: #804000"
     misc_feature    607..639
                     /label=GBP PCR Primer F'
                     /note="color: #a6acb3"
     CDS             622..969
                     /codon_start=1
                     /label=GFP Binding Protein (GBP)
                     /note="color: #00ccff"
                     /translation="MQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKE
                     REWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFE
                     YWGQGTQVTVSS"
     misc_feature    952..987
                     /label=GBP PCR Primer R'
                     /note="color: #a6acb3"
     misc_feature    973..983
                     /label=MCS
                     /note="color: #993300"
     CDS             991..1671
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /note="color: #ff9900"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(1061..1080)
                     /label=mEos2-N Primer
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     1489..1508
                     /label=mEos2-C Primer
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    1809..1930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #8000ff"
     rep_origin      complement(1937..2392)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffeaea"
     promoter        2419..2523
                     /label=AmpR promoter
                     /note="color: #8080ff; direction: RIGHT"
     promoter        2525..2882
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ff6c6c; direction: RIGHT"
     rep_origin      2733..2868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #cc0000"
     CDS             2917..3711
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #faffdd"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3943..3990
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
                     /note="color: #804000"
     rep_origin      4319..4907
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #a4a400"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct catgcaggtt caactggtgg aaagcggcgg tgctctggta
      661 caaccgggcg gtagtctgcg cctgagctgt gccgcaagcg gtttcccagt caaccgctac
      721 tctatgcgtt ggtatcgcca ggcgcctggt aaagaacgtg aatgggttgc cggcatgagc
      781 agtgcgggcg atcgttctag ttacgaggac tctgttaaag gtcgttttac aattagccgt
      841 gatgatgcgc gcaataccgt gtatctgcaa atgaacagtc tgaagccgga ggacaccgca
      901 gtatattatt gcaatgtcaa cgtggggttt gaatattggg gccaggggac tcaggtgacg
      961 gtgagctcta aagatccacc ggtcgccacc atgagtgcga ttaagccaga catgaagatc
     1021 aaactccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
     1081 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
     1141 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccaaa
     1201 tatccagaca acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     1261 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa
     1321 ggggacactt tctataataa agttcgattt tatggtacca actttcccgc caatggtcca
     1381 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     1441 ggagtgctga cgggtgatat tcatatggct ttgttgcttg aaggaaatgc ccattaccga
     1501 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
     1561 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     1621 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata agagctgtac
     1681 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     1741 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1801 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1861 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1921 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     1981 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2041 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2101 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2161 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2221 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2281 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2341 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     2401 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2461 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2521 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     2581 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     2641 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2701 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     2761 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     2821 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     2881 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     2941 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     3001 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     3061 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     3121 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     3181 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     3241 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     3301 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     3361 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     3421 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     3481 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     3541 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     3601 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     3661 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     3721 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     3781 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     3841 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     3901 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     3961 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     4021 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     4081 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     4141 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     4201 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4261 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4321 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4381 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4441 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4501 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4561 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4621 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4681 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4741 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4801 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4861 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4921 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4981 tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
