LOCUS       Exported                7309 bp ds-DNA     circular SYN 05-NOV-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Institute for Molecular Bioscience
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 5, 2020 from SnapGene 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    519..539
                     /label=CMV F primer
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             613..1290
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /note="color: #ff9900"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    complement(683..702)
                     /label=mEos2 R primer
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    1111..1130
                     /label=mEos2 F primer
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1303..1317
                     /label=MCS
                     /note="color: #a6acb3"
     misc_feature    1318..3936
                     /label=PA-PLA1 ORF 2619bp
                     /note="color: #3366ff"
     misc_feature    3937..3973
                     /label=MCS (part2)
                     /note="color: #a6acb3"
     polyA_signal    4097..4218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    complement(4134..4153)
                     /label=SV40pA-R primer
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      complement(4225..4680)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4707..4811
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4813..5170
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5021..5156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5205..5999
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6231..6278
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     rep_origin      6607..7195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc
      661 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      721 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      781 ctgaccactg cattccatta cggcaacagg gtattcgcca aatatccaga caacatacaa
      841 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      901 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      961 aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg
     1021 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat
     1081 attcatatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1141 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1201 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1261 cattctggat tgcctgacaa tgccagacga gagctgtaca agtccggact cagatctatg
     1321 aattacccgg gccgcgggtc cccacggagc cccgagcata acggccgagg cggcggcggc
     1381 ggcgcctggg agctgggctc agacgcgagg ccagcgttcg gcggcggcgt ctgctgcttc
     1441 gagcacctgc ccggcgggga cccggacgac ggcgacgtgc ccctggccct gctgcgcggg
     1501 gaacccgggc tgcatttggc gccgggcacc gacgaccaca accaccacct cgcgctggac
     1561 ccctgcctca gtgacgagaa ctatgacttc agctccgccg agtcgggctc ctcgctgcgc
     1621 tactacagcg agggtgagag cggcggcggc ggcagctcct tgtcgctgca cccgccgcag
     1681 cagcctccgc tggtcccgac gaactcgggg ggcggcggcg cgacaggagg gtcccccggg
     1741 gaaaggaaac gtacccggct tggcggcccg gcggcccggc accgctatga ggtagtgacg
     1801 gagctgggcc cggaggaggt acgctggttc tacaaggagg acaagaagac ctggaagccc
     1861 ttcatcggct acgactcgct ccgcatcgag ctcgccttcc ggaccctgct gcagaccacg
     1921 ggtgcccggc cccagggcgg ggaccgggac ggcgaccatg tgtgctcccc cacgggccca
     1981 gcctccagtt ccggagaaga tgacgatgag gaccgcgcct gcggcttctg ccagagtacg
     2041 acggggcacg agccggagat ggtggagctt gtgaacatcg agcctgtgtg cgtgcggggc
     2101 ggcctctacg aggtggatgt gacccaagga gagtgctacc cggtgtactg gaaccaggct
     2161 gataaaatac cagtaatgcg tggacagtgg tttattgacg gcacttggca gcctctagaa
     2221 gaggaagaaa gtaatttaat tgagcaagaa catctcaatt gttttagggg ccagcagatg
     2281 caggaaaatt tcgatattga agtgtcaaaa tccatagatg gaaaagatgc tgttcatagt
     2341 ttcaagttga gtcgaaacca tgtggactgg cacagtgtgg atgaagtata tctttatagt
     2401 gatgcaacaa catctaaaat tgcaagaaca gttacccaaa aactgggatt ttctaaagca
     2461 tcaagtagtg gtaccagact tcatagaggt tatgtagaag aagccacatt agaagacaag
     2521 ccatcacaga ctacccatat tgtatttgtt gtgcatggca ttgggcagaa aatggaccaa
     2581 ggaagaatta tcaaaaatac agctatgatg agagaagctg caagaaaaat agaagaaagg
     2641 catttttcca accatgcaac acatgttgaa tttctgcctg ttgagtggcg gtcaaaactt
     2701 actcttgatg gagacactgt tgattccatt actcctgaca aagtacgagg tttaagggat
     2761 atgctgaaca gcagtgcaat ggacataatg tattatacta gtccacttta tagagatgaa
     2821 ctagttaaag gccttcagca agagctgaat cgattgtatt cccttttctg ttctcggaat
     2881 ccagactttg aagaaaaagg gggtaaagtc tcaatagtat cacattcctt gggatgtgta
     2941 attacttatg acataatgac tggctggaat ccagttcggc tgtatgaaca gttgctgcaa
     3001 aaggaagaag agttgcctga tgaacgatgg atgagctatg aagaacgaca tcttcttgat
     3061 gaactctata taacaaaacg acggctgaag gaaatagaag aacggcttca cggattgaaa
     3121 gcatcatcta tgacacaaac acctgcctta aaatttaagg ttgagaattt cttctgtatg
     3181 ggatccccat tagcagtttt cttggcgttg cgtggcatcc gcccaggaaa tactggaagt
     3241 caagaccata ttttgcctag agagatttgt aaccggttac taaatatttt tcatcctaca
     3301 gatccagtgg cttatagatt agaaccatta atactgaaac actacagcaa catttcacct
     3361 gtccagatcc actggtacaa tacttcaaat cctttacctt atgaacatat gaagccaagc
     3421 tttctcaacc cagctaaaga acctacctca gtttcagaga atgaaggcat ttcaaccata
     3481 ccaagccctg tgacctcacc agttttgtcc cgccgacact atggagaatc tataacaaat
     3541 ataggcaaag caagcatatt aggggctgct agcattggaa agggacttgg aggaatgttg
     3601 ttctcaagat ttggacgttc atctacaaca cagtcatctg aaacatcaaa agactcaatg
     3661 gaagatgaga agaagccagt tgcctcacct tctgctacca ccgtagggac acagaccctt
     3721 ccacatagca gttctggctt cctcgattct gcattggagt tggatcacag gattgatttt
     3781 gaactcagag aaggccttgt ggagagccgc tattggtcag ctgtcacgtc gcatactgcc
     3841 tattggtcat ccttggatgt tgcccttttt cttttaacct tcatgtataa acatgagcac
     3901 gatgatgatg caaaacccaa tttagatcca atctgagaat tctgcagtcg acggtaccgc
     3961 gggcccggga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     4021 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     4081 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4141 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4201 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     4261 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     4321 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     4381 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4441 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     4501 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     4561 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     4621 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     4681 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4741 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4801 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     4861 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4921 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4981 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5041 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5101 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5161 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5221 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5281 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5341 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     5401 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5461 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5521 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5581 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5641 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     5701 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     5761 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5821 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     5881 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     5941 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6001 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6061 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6121 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     6181 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     6241 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     6301 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     6361 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     6421 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     6481 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6541 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6601 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6661 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6721 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6781 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6841 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6961 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7021 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7081 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7141 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7201 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7261 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
