LOCUS       Exported                3986 bp ds-DNA     circular SYN 22-OCT-2020
DEFINITION  B4-hr5-ie1p>-B1r.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 29, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     Insert Molecule:   C413-P45, 1139 bps DNA (attB4, attB1r sites)
            Donor Vector:   pDonr-233, 5033 bps DNA circular (attP4, attP1r 
            sites)
            Recombination using BP clonase to produce Entry Clone
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    110..205
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    208..720
                     /label=hr5
     promoter        697..1289
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     protein_bind    1292..1416
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1489..1507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1512..1528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1953..2744
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2837..3425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3550..3603
                     /label=pas
                     /note=" pBR primosome ass'y site (L)"
     terminator      3755..3782
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      3874..3960
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgatcg ggcccgcgtt aacgctacca tggagctcca aataatgatt
      121 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata
      181 atgccaactt tgtatagaaa agttggcgcc gcgtaaaaca caatcaagta tgagtcataa
      241 gctgatgtca tgttttgcac acggctcata accgaactgg ctttacgagt agaattctac
      301 ttgtaacgca cgatcgagtg gatgatggtc atttgttttt caaatcgaga tgatgtcatg
      361 ttttgcacac gggctcataa actgctttac gagtagaatt ctacgtgtaa cgcacgatcg
      421 attgatgagt catttgtttt gcaatatgat atcatacaat atgactcatt tgtttttcaa
      481 aaccgaactt gatttacggg tagaattcta ctcgtaaagc acaatcaaaa agatgatgtc
      541 atttgttttt caaaactgaa ctctcggctt tacgagtaga attctacgtg taaaacacaa
      601 tcaagaaatg atgtcatttg ttataaaaat aaaagctgat gtcatgtttt gcacatggct
      661 cataactaaa ctcgctttac gggtagaatt ctacgtcgat gtctttgtga tgcgccgaca
      721 tttttgtagg ttattgataa aatgaacgga tacagttgcc cgacattatc attaaatcct
      781 tggcgtagaa tttgtcgggt ccattgtccg tgtgcgctag catgcccgct aacggacctc
      841 gtacttttgg cttcaaaggt tttgcgcaca gacaaaatgt gccacacttg cagctctgca
      901 tgtgtgcgcg ttaccacaaa tcccaacggc gcagtgtact tgttgtatgc aaataaatct
      961 cgataaaggc gcggcgcgcg aatgcagctg atcacgtacg ctcctcgtgt tccgttcaag
     1021 gacggtgtta tcgacctcag attaatgttt atcggccgac tgttttcgta tccgctcacc
     1081 aaacgcgttt ttgcattaac attgtatgtc ggcggatgtt ctatatctaa tttgaataaa
     1141 taaacgataa ccgcgttggt tttagagggc ataataaaag aaatattgtt atcgtgttcg
     1201 ccattagggc agtataaatt gacgttcatg ttggatattg tttcagttgc aagttgacac
     1261 tggcggcgac aagatcgtga acaaccaagt gacaactttg tacaaaaaag ttgaacgaga
     1321 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1381 ataatactgt aaaacacaac atatgcagtc actatgaacc aactacttag atggtattag
     1441 tgacctgtag aattcgagct ctagagctgc agggcggccg cgatatcccc tatagtgagt
     1501 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     1561 gttacattgc acaagatcgg tacccgggga tcctctagac cagccaggac agaaatgcct
     1621 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     1681 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     1741 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     1801 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     1861 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     1921 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     1981 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2041 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     2101 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2161 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2221 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2281 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2341 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2401 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2461 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2521 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2581 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     2641 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     2701 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     2761 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     2821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     3241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3481 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3541 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3601 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3661 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     3721 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3781 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3841 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3901 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     3961 atgcctggca gttccctact ctcgcg
//
