LOCUS       Exported                3623 bp ds-DNA     circular SYN 23-OCT-2020
DEFINITION  B5r-HT7-B1r.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 30, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  235 cut
               Fragment ends: attB1 and attB5
               Fragment size: 2659
            Insert molecule:  Fragment 2
               Fragment ends: attB5 and attB1
               Fragment size: 964
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(190..208)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(213..229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             654..1445
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1538..2126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2261..2288
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2380..2466
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(2550..2673)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2677..3567
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)-
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     protein_bind    join(3616..3623,1..117)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
       61 tttgcataaa aaacagacta cataatactg taaaacacaa catatgcagt cactatgaac
      121 caactactta gatggtatta gtgacctgta gaattcgagc tctagagctg cagggcggcc
      181 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
      241 ccgtgtctca aaatctctga tgttacattg cacaagatcg gtacccgggg atcctctaga
      301 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
      361 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
      421 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      481 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
      541 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
      601 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
      661 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      721 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      781 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
      841 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
      901 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
      961 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     1021 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     1081 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     1141 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     1201 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1261 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1321 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     1381 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     1441 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     1501 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1561 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1621 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1681 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1741 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1801 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1861 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1921 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1981 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2041 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2101 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2161 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2221 accgtattac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     2281 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     2341 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     2401 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     2461 tatttgatgc ctggcagttc cctactctcg cgttaacgct accatggagc gaattctaca
     2521 ggtcactaat accatctaag tagttgattc atagtgactg catatgttgt gttttacagt
     2581 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     2641 acgtttctcg ttcaactttt gtatacaaag tggaccatgg cagaaatcgg tactggcttt
     2701 ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt
     2761 ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg
     2821 cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga cctgatcggt
     2881 atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt ccgcttcatg
     2941 gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca cgactggggc
     3001 tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg tattgcattt
     3061 atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc ccgcgagacc
     3121 ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca gaacgttttt
     3181 atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat
     3241 taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt cccaaacgag
     3301 ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata catggactgg
     3361 ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt tctgatccca
     3421 ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc
     3481 ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga gatcgcgcgc
     3541 tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta ctttcagagc
     3601 gataacgcga tcgccacaac ttt
//
