LOCUS       Exported                3022 bp ds-DNA     circular SYN 28-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Vanessa Wall
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 30, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      14..100
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(184..307)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             311..579
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             589..615
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             619..645
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    649..773
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(846..864)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(869..885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1310..2101
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2194..2782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2917..2944
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
       61 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      121 ctcgcgttaa cgctaccatg gagcgaattc tacaggtcac taataccatc taagtagttg
      181 attcatagtg actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      241 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac ttttgtatac
      301 aaagtggcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
      361 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
      421 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
      481 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
      541 cgtctttgtt tcgttttctg ttctgcgccg ttacagatca ccatgggcta cccctatgat
      601 gttccagatt acgctggata tccatacgac gtaccggact atgcgggtac aactttgtac
      661 aaaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      721 cataaaaaac agactacata atactgtaaa acacaacata tgcagtcact atgaaccaac
      781 tacttagatg gtattagtga cctgtagaat tcgagctcta gagctgcagg gcggccgcga
      841 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
      901 gtctcaaaat ctctgatgtt acattgcaca agatcggtac ccggggatcc tctagaccag
      961 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     1021 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     1081 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1141 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     1201 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1261 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     1321 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1381 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1441 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1501 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1561 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     1621 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     1681 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     1741 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     1801 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     1861 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     1921 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     1981 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2041 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2101 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2341 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2581 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2881 tattaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     2941 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     3001 cgttgcttca caacgttcaa at
//
