LOCUS       Exported                3049 bp ds-DNA     circular SYN 23-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 30, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    complement(79..202)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             206..474
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             484..567
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag-
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     protein_bind    571..695
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(768..786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(791..807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1232..2023
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2116..2704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2839..2866
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2958..3044
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tggcagttcc ctactctcgc gttaacgcta ccatggagcg aattctacag gtcactaata
       61 ccatctaagt agttgattca tagtgactgc atatgttgtg ttttacagta ttatgtagtc
      121 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
      181 tcaacttttg tatacaaagt ggcgaggggc tcgcatctct ccttcacgcg cccgccgccc
      241 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
      301 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
      361 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
      421 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatcaccatg
      481 gggtggtcac acccgcaatt cgaaaaggga ggcggttctg gaggcggatc cggaggctcg
      541 gcttggagcc atcctcagtt cgagaagggg acaactttgt acaaaaaagt tgaacgagaa
      601 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
      661 taatactgta aaacacaaca tatgcagtca ctatgaacca actacttaga tggtattagt
      721 gacctgtaga attcgagctc tagagctgca gggcggccgc gatatcccct atagtgagtc
      781 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
      841 ttacattgca caagatcggt acccggggat cctctagacc agccaggaca gaaatgcctc
      901 gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
      961 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     1021 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     1081 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     1141 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     1201 gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc
     1261 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     1321 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     1381 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     1441 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     1501 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     1561 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     1621 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     1681 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     1741 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1801 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1861 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1921 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1981 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg
     2041 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     2101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2221 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     2281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     2521 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2701 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2761 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ctagccagga
     2821 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
     2881 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     2941 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     3001 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcc
//
