LOCUS       Exported                3356 bp ds-DNA     circular SYN 23-OCT-2020
DEFINITION  attL4/attR1 vector, murine RNA Pol2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 29, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    94..189
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        198..902
                     /gene="pPol2"
                     /label=mpPol2
                     /note="murine RNAPol2 promoter"
     protein_bind    903..1026
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1099..1117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1122..1138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1251..2060
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2207..2795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      3125..3152
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      3244..3330
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
       61 ctcgggcccg cgttaacgct accatggagc tccaaataat gattttattt tgactgatag
      121 tgacctgttc gttgcaacaa attgataagc aatgcttttt tataatgcca actttgtata
      181 gaaaagttgg caagcttggg cttgaacatc gagcgccagg gctccgtaaa gctactagag
      241 cacaggcggt gccccaacgt cctggggcct ctccactaat aacggctact tccaattgat
      301 tggacgcgcc atcttgcctg ccttatgcat attcagcggt gaactgaata ttcatgaacg
      361 aggcccgtcc cgtccctccc tccttccccc cacccccgga acccgctccg gaggacccga
      421 agggccccgc cttcattacc gatgcgtagg acaaaccatt ttcccgatgt gtgtgggggg
      481 atactaatga gagactttag ctgaaaatga gcctgaactc cgaagctgag taaaaatggc
      541 ctaactttat cctccgttct gtaagtcctc ggtttgagtg cacgggaaac ccgaaaggag
      601 gacgacagga ccaggaacat tctcctcctc ctgtcgcgtc agaaagaaca cccaaccagg
      661 gagccggagc cctagcgtca acaactccgc cgcgcgcgct ccgtgtaggc cggtgcgggc
      721 ggccccgtag cgcaagggag ggcgggaaag gaaggggcgg gacacaaggg cgaatctata
      781 aagggcgtca ctcagccagt tctctcctca gaagcgccga gagcgcgacc gggacggttg
      841 gagaagaagg tggctcccgg aagggggaga gacaaactgc cgtaacctct gccgttcagg
      901 atcaactttg tacaaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt
      961 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatgcagtc
     1021 actatgaatc aactacttag atggtattag tgacctgtag aattcgagct ctagagctgc
     1081 agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     1141 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1201 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     1261 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     1321 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     1381 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     1441 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     1501 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     1561 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     1621 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     1681 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     1741 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     1801 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     1861 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     1921 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     1981 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     2041 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     2101 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     2161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2341 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2581 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2821 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2881 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2941 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3001 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3061 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3121 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3181 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3241 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3301 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
