LOCUS       Exported                3463 bp ds-DNA     circular SYN 22-OCT-2020
DEFINITION  attL4/attL5 vector, human ubiquitin C promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 29, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    7..102
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        102..1313
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     protein_bind    complement(1317..1412)
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1460..1546
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1638..1665
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1800..2388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2531..3340)
                     /codon_start=1
                     /label=KanR
                     /note="kanamycin resistance"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 agctccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
       61 agcaatgctt ttttataatg ccaactttgt atagaaaagt tggcctccgc gccgggtttt
      121 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
      181 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
      241 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
      301 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
      361 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
      421 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
      481 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
      541 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
      601 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
      661 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
      721 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
      781 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
      841 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
      901 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
      961 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     1021 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     1081 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     1141 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     1201 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     1261 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacggccaac
     1321 ttttgtatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca
     1381 ggtcactatc agtcaaaata aaatcattat ttggagctcc atggtagcgt taacgcgaga
     1441 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcggaagact gggcctttcg
     1501 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     1561 tttgaacgtt gtgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     1621 caggcatcaa actaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     1681 tcttcctggc tagcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1741 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1801 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1861 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1921 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1981 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2041 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2101 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2161 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2221 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2281 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2341 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2401 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2461 atgagcttgc gccgtcccgt caagtcagcg taatgctctg ccggtgttac aaccaattaa
     2521 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     2581 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     2641 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     2701 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     2761 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt
     2821 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     2881 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     2941 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     3001 aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca gtggtgagta
     3061 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     3121 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     3181 gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg
     3241 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     3301 ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     3361 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     3421 atcagagatt ttgagacacg ggccctgcgc cctgcagctc tag
//
