LOCUS       Exported                2402 bp ds-DNA     circular SYN 23-OCT-2020
DEFINITION  attL4/attL5 vector, stuffer fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 29, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    7..102
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             105..255
                     /label=stuffer
                     /note="stuffer control fragment"
     protein_bind    complement(256..351)
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      399..485
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      577..604
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(739..1327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1470..2279)
                     /codon_start=1
                     /label=KanR
                     /note="kanamycin resistance"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 agctccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
       61 agcaatgctt ttttataatg ccaactttgt atagaaaagt tggccccgaa ggctacgtcc
      121 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      181 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      241 gcaacatcct ggggacaact tttgtataca aagttggcat tataaaaaag cattgcttat
      301 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tggagctcca
      361 tggtagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      421 cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      481 caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag
      541 gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc
      601 tttttgcgtt tctacaaact cttcctggct agcggtaata cggttatcca cagaatcagg
      661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     1201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1381 acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc
     1441 cggtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     1501 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1561 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1621 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1681 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     1741 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1801 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1861 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1921 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgtttttccg
     1981 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2041 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     2101 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaag
     2161 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     2221 tcagcatcca tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata
     2281 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     2341 ttatcttgtg caatgtaaca tcagagattt tgagacacgg gccctgcgcc ctgcagctct
     2401 ag
//
