LOCUS       Exported                7286 bp ds-DNA     circular SYN 29-OCT-2020
DEFINITION  367 bp Mlu1/EcoR1 Alb-AAT GeneBlock added to #413/P9-1. Replaces 
            EF-1a with Alb-AAT promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Jon Backstrom
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 29, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     Sequencing (5-Cas-Seq-A2 and SVpa-Seq-S)
            Clones 1 and 2 OK
            
            Use clone-1 for Maxi-prep (9/16/19)
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /label=Left ITR
                     /note="color: #3366ff"
     misc_feature    152..171
                     /label=C/EBP-A
                     /note="color: #ff00ff"
     misc_feature    192..211
                     /label=C/EBP-B
                     /note="color: #ff00ff"
     misc_feature    303..333
                     /label=eH region
                     /note="Ref 2"
                     /note="color: #------"
     misc_feature    306..315
                     /label=eH-TF
                     /note="color: #ff00ff"
     misc_feature    361..461
                     /label=TSE
                     /note="-137 to -37"
                     /note="color: #------"
     misc_feature    377..395
                     /label=HNF-4
                     /note="color: #00ff00"
     misc_feature    415..427
                     /label=HNF-1
                     /note="color: #00ff00"
     misc_feature    471..477
                     /label=TATA
                     /note="color: #ffff00"
     misc_feature    499..505
                     /label=mRNA
                     /note="color: #ffffff; direction: RIGHT"
     CDS             521..706
                     /codon_start=1
                     /gene="ZNF10"
                     /gene_synonym="KOX1"
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /note="color: #993366"
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     misc_feature    707..727
                     /label=GS linker
                     /note="color: #------; direction: RIGHT"
     CDS             728..3889
                     /codon_start=1
                     /label=h-SadCas9
                     /note="color: #993366"
                     /translation="MGKRNYILGLAIGITSVGYGIIDYETRDVIDAGVRLFKEANVENN
                     EGRRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEE
                     EFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDG
                     EVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPF
                     GWKDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEK
                     FQIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARK
                     EIIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLK
                     AINLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQS
                     IKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKEN
                     AKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDAIIPRSVSFDNSFNNKVLV
                     KQEENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINR
                     FSVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKE
                     RNKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKE
                     IFITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDK
                     DNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSK
                     KDNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVK
                     NLDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDL
                     LNRIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHP
                     QIIKKG"
     primer_bind     complement(739..759)
                     /label=5-Cas-Seq-A
                     /note="58/23"
                     /note="color: black; sequence: GCAAGCCCCAGGATGTAGTTC"
     primer_bind     complement(778..806)
                     /label=5-Cas-Seq-A2
                     /note="57/22"
                     /note="color: black; sequence: 
                     GTGTCTCATAATCAATAATTCCGTAGCCA"
     primer_bind     complement(1238..1266)
                     /label=SnaB-anti
                     /note="color: black; sequence: 
                     TCCTTTACGTAATCTGAAGTCTTGAACCT; added: 2019-02-23"
     primer_bind     complement(1310..1335)
                     /label=SnaB-Seq-A
                     /note="IDT-25"
                     /note="color: black; sequence: ATGTAGGTATCGATGAAGCTCTGATC"
     misc_feature    2308..2318
                     /label=BstAP1
                     /note="color: #ffffff"
     primer_bind     2734..2763
                     /label=BbvC1-Seq-S
                     /note="59/33"
                     /note="color: black; sequence: 
                     TCTGGACGTTAAAGTCAAGAGCATAAATGG"
     primer_bind     2832..2856
                     /label=BbvC1-Cas9-S
                     /note="60/31"
                     /note="color: black; sequence: atgctgaggatgctttgatcatcgc"
     misc_feature    3773..3775
                     /label=Arg2015
                     /note="color: #ff0000"
     misc_feature    3776..3787
                     /label=CTD beta-7 Strand
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    3809..3826
                     /label=CTD beta-8 Strand
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    3812..3814
                     /label=Delta-1028
                     /note="color: #cc99ff"
     primer_bind     3816..3842
                     /label=Cas9-C-Seq-S
                     /note="color: black; sequence: AGTACTCTACCGATATCCTGGGGAATC;
                     added: 2019-04-16"
     misc_feature    3827..3832
                     /label=EcoRV
                     /note="First site just downstream of Afl2"
                     /note="color: #a6acb3"
     misc_feature    3827..3829
                     /label=Delta-1033
                     /note="color: #cc99ff"
     misc_feature    3836..3838
                     /label=Delta-1036
                     /note="color: #cc99ff"
     misc_feature    3842..3850
                     /label=CTD beta-9 Strand
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    3869..3871
                     /label=Delta-1047
                     /note="color: #cc99ff"
     primer_bind     3888..3907
                     /label=[Bam-350-sense]
                     /note="60/40"
                     /note="color: black; sequence: GTGGATCCGCAGAAGCAGCA"
     misc_feature    3896..3946
                     /label=EA Linker
                     /note="Bam/Age1"
                     /note="color: #------"
     CDS             3959..3985
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     primer_bind     3980..4002
                     /label=Not-pa-gRNA-S
                     /note="61/42Used to amplify Not-Avr2 to add U6-gRNA to SB"
                     /note="color: black; sequence: CTAGATTAGcggccgcatcgtca; 
                     added: 2019-02-13"
     misc_feature    3988..3995
                     /label=Not1
                     /note="1 of 3; others at ITRs"
                     /note="color: #ffffff"
     polyA_signal    4029..4150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     4050..4074
                     /label=SVpA-seq-S
                     /note="color: black; sequence: GACAAACCACAACAAGAATGCAGTG; 
                     added: 2019-04-09"
     primer_bind     complement(4050..4073)
                     /label=SVpa-seq-A
                     /note="56/19IDT-29"
                     /note="color: black; sequence: ACTGCATTCTTGTTGTGGTTTGTC"
     primer_bind     complement(4158..4188)
                     /label=Kpn-SB-A
                     /note="color: black; sequence: 
                     AGGTACCTGAAACATAAAATGAATGCAATTGT"
     promoter        4189..4429
                     /label=hU6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #99ccff; direction: RIGHT"
     misc_feature    4217..4224
                     /label=Octamer
                     /note="color: #------"
     misc_feature    4373..4390
                     /label=PSE
                     /note="color: #------"
     misc_feature    4438..4459
                     /label=mPcsk9 gRNA
                     /note="S = 60/15
                     A = 60/4
                     Anneal: 60/31"
                     /note="color: #ff0000"
     misc_RNA        4460..4535
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
                     /note="color: #cc99ff; direction: RIGHT"
     primer_bind     complement(4523..4546)
                     /label=Avr2-gRNA-A
                     /note="color: black; sequence: 
                     ACCTAGGCCGCAAAAATCTCGCCAACAAG; added: 2019-02-20"
     misc_feature    4541..4548
                     /label=Not1
                     /note="color: #ffffff"
     repeat_region   4549..4689
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #3366ff"
     rep_origin      4764..5219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5501..5605
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5606..6466
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5606 .. 5674 / #ccffcc / signal sequence
                        2: 5675 .. 6466 / #ccffcc
                     Cleavage site after base 5674"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6637..7225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaaataa cctgcgttat agcatccact cagtatccct
      181 tgagcatgag gtgacactac ttaacatagg gacgagatgg tactttgtgt ctcctgctct
      241 gtcagcaggg cactgtactt gctgatacag ggaatgtttg ttcttaaata ccatcattcc
      301 ggacgtgttt gccttggcag tttccatgta catgcagaaa gaagtttgga taggcgggcg
      361 actcagatcc cagccagtgg acttagcccc tgtttgctcc tccgataact ggggtgacct
      421 tggttaatat tcaccagcag cctcccccgt tgcccctctg gatccactgc ttaaatacgg
      481 acgaggacag ggccctgtct cctcagaatt cgccaccatg cggacactag tgaccttcaa
      541 ggatgtattt gtggacttca ccagggagga gtggaagctg ctggacactg ctcagcagat
      601 cgtgtacaga aatgtgatgc tggagaacta taagaacctg gtttccttgg gttatcagct
      661 tactaagcca gatgtgatcc tccggttgga gaagggagaa gagcccggcg gttccggcgg
      721 agggtccatg ggcaaacgga actacatcct ggggcttgcc attgggataa ccagcgttgg
      781 ctacggaatt attgattatg agacacgcga tgtgattgac gccggggtta ggctgttcaa
      841 agaggccaac gttgaaaaca acgagggaag acggagtaag cgcggagcaa gaagactcaa
      901 gcgcagacgg agacatcgga ttcagagggt gaaaaagctg ctcttcgatt acaatctcct
      961 gaccgatcat agtgagctga gcggaatcaa cccctacgag gcgcgagtga aagggctttc
     1021 ccagaagctg tccgaagagg agttctccgc cgcgttgctg cacctggcca aacggagggg
     1081 ggttcacaat gtaaacgaag tggaggagga cacgggcaat gaacttagta cgaaagaaca
     1141 gatcagtagg aactctaagg ctctcgaaga gaaatacgtc gctgagttgc agcttgagag
     1201 actgaaaaaa gacggcgaag tacgcggatc tattaatagg ttcaagactt cagattacgt
     1261 aaaggaagcc aagcagctcc tgaaagtaca gaaagcgtac catcagctcg atcagagctt
     1321 catcgatacc tacatagatt tgctggagac acggaggaca tactacgagg gcccagggga
     1381 aggatctcct tttgggtgga aggacatcaa ggaatggtac gagatgctta tgggacattg
     1441 tacatatttt ccggaggagc tcaggagcgt caagtacgcc tacaatgccg acctgtacaa
     1501 tgccctcaat gacctcaata acctcgtgat taccagggac gagaacgaga agctggagta
     1561 ctatgaaaag ttccagatta tcgagaatgt gtttaagcag aagaagaagc cgacacttaa
     1621 gcagattgca aaggaaatcc tcgtgaatga ggaagatatc aagggataca gagtgacaag
     1681 tacaggcaag cccgagttca caaatctgaa ggtgtaccac gatattaagg acataaccgc
     1741 acgaaaggag ataatcgaaa acgctgagct cctcgatcag atcgcaaaaa ttcttaccat
     1801 ctaccagtct agtgaggaca ttcaggagga actgactaat ctgaacagtg agctcaccca
     1861 agaggaaatt gagcagattt caaacctgaa aggctacacc gggacgcaca atctgagcct
     1921 caaagcaatc aacctcattc tggatgaact ttggcacaca aatgacaacc aaattgccat
     1981 attcaaccgc ctgaaactgg tgccaaaaaa agtggatctg tcacagcaaa aggaaatccc
     2041 tacaaccttg gttgacgatt ttattctgtc ccccgttgtc aagcggagct tcatccagtc
     2101 aatcaaggtg atcaatgcca tcattaaaaa atacggattg ccaaacgata taattatcga
     2161 gcttgcacga gagaagaact caaaggacgc ccagaagatg attaacgaaa tgcagaagcg
     2221 caaccgccag acaaacgaac gcatagagga aattataaga acaaccggca aagagaatgc
     2281 caagtatctg atcgagaaaa tcaagctgca cgacatgcaa gaaggcaagt gcctgtactc
     2341 tctggaagct atcccactcg aagatctgct gaataatcca ttcaattacg aggtggacgc
     2401 catcatccct agatccgtaa gctttgacaa ttccttcaat aacaaagttc tggttaaaca
     2461 ggaggaaaat tctaaaaaag ggaaccggac cccgttccag tacctgagct ccagtgacag
     2521 caagattagc tacgagactt ttaagaaaca tattctgaat ctggccaaag gcaaaggcag
     2581 gatcagcaag accaagaagg agtacctcct cgaagaacgc gacattaaca gatttagtgt
     2641 gcagaaagat ttcatcaacc gaaaccttgt cgatactcgg tacgccacga gaggcctgat
     2701 gaatctcctc aggagctact tccgcgtcaa taatctggac gttaaagtca agagcataaa
     2761 tgggggattc accagctttc tgaggagaaa gtggaagttt aagaaggaac gaaacaaagg
     2821 atacaagcac catgctgagg atgctttgat catcgctaac gcggacttta tctttaagga
     2881 atggaaaaag ctggataagg caaagaaagt gatggaaaac cagatgttcg aggagaagca
     2941 ggcagagtca atgcctgaga tcgagacaga gcaggaatac aaggaaattt tcatcacccc
     3001 tcatcagatt aaacacataa aggacttcaa agactataaa tactctcata gggtggacaa
     3061 aaaacccaat cgcgagctca ttaatgacac cctgtactca acacggaagg atgataaagg
     3121 taataccttg attgtgaata atcttaatgg attgtatgac aaagataacg acaagctcaa
     3181 gaagctgatc aacaagtctc cagagaagct ccttatgtat caccacgacc cacagactta
     3241 tcagaaattg aaactgatca tggagcaata cggggatgag aagaacccac tctacaaata
     3301 ttatgaggaa acaggtaatt acctgaccaa gtactccaag aaggataacg gaccagtgat
     3361 caaaaagata aagtactatg gcaacaaact taatgcgcat ttggacataa ctgacgatta
     3421 ccccaattct cgaaacaagg ttgtgaagct ctccctgaag ccttatagat ttgacgtgta
     3481 cctggataat ggggtttata aattcgtcac cgtgaaaaat ctggacgtga tcaaaaagga
     3541 gaactattat gaagtaaact caaagtgcta tgaggaggcg aagaagctga agaagatctc
     3601 caatcaggcc gagttcatcg cttccttcta taataacgat ctcatcaaga tcaatggaga
     3661 gctttatcgc gtcattggtg tgaacaatga cttgctgaac aggatcgaag tcaatatgat
     3721 agacattacc taccgggagt atctcgaaaa catgaatgat aaacggccgc ctcgcatcat
     3781 caagacaatc gcatctaaaa ctcagtcaat aaaaaagtac tctaccgata tcctggggaa
     3841 tctctatgaa gtgaagtcaa agaagcaccc acaaatcatt aaaaaaggtg gatccgcaga
     3901 agcagcagca aaggaagcag cagcaaagga agcagcagca aaggcagtcg acaccggtcc
     3961 agcagctaag agagttaaac tagattagcg gccgcatcgt caggccaagc ttccatcgat
     4021 agacatgata agatacattg atgagtttgg acaaaccaca acaagaatgc agtgaaaaaa
     4081 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4141 taaacaagtt aacaacaaca attgcattca ttttatgttt caggtaccga gggcctattt
     4201 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
     4261 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
     4321 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
     4381 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgag
     4441 ggaagggata caggctggag ttttagtact ctggaaacag aatctactaa aacaaggcaa
     4501 aatgccgtgt ttatctcgtc aacttgttgg cgagattttt gcggccgcag gaacccctag
     4561 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     4621 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     4681 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     4741 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4801 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4861 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4921 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     4981 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5041 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5101 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5161 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     5221 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     5281 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5341 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5401 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5461 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5521 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5581 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5641 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5701 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5761 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5821 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5881 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5941 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6001 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6061 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6121 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6181 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6241 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6301 atctggagcc ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa
     6361 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6421 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6481 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6541 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6601 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6661 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6721 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6781 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6841 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6901 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6961 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7021 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7081 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7141 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7201 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7261 cttttgctgg ccttttgctc acatgt
//
