LOCUS       Exported                6658 bp ds-DNA     circular SYN 30-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 30, 2020 from SnapGene Viewer 5.2.0
            https://www.snapgene.com
COMMENT     Vector CMS521 digested with XbaI, NheI, BamHI, 2 bands released 750,
            450
            f1:CGACCTCGGGCTCGGGCagcggtggcTCTAGAG
            r1:GCTcacAAAAATcacTGGGTTGTCAGGCAG
            f2:CTGCCTGACAACCCAgtgATTTTTgtgAGC
            r2:CTTATCGTCGTCATCCTTGTAGTCggatcc
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /label=ITR
     misc_feature    151..708
                     /label=CMV promoter
     misc_feature    753..769
                     /label=T7 promoter primer
     misc_feature    785..786
                     /label=NotI
     misc_feature    791..853
                     /label=Ig k-chain signal sequence
     misc_feature    869..874
                     /label=BglII
     misc_feature    875..901
                     /label=HA tag
     misc_feature    929..1606
                     /label=human CD4
     misc_feature    1607..1610
                     /label=10 aa linker
     misc_feature    1611..1627
                     /label=annealing region for overlap
     misc_feature    1611..1627
                     /label=10 aa linker
                     /label=10 aa linker(2)
     misc_feature    1628..1636
                     /label=10 aa linker
                     /label=10 aa linker(1)
     misc_feature    1676..2188
                     /label=CIBN
     misc_feature    join(2204..2224,2228..2251,2255..2269)
                     /label=M13O (CaMKII)
     misc_feature    2342..2377
                     /label=NNES
     misc_feature    2384..2389
                     /label=nhe1
     misc_feature    2384..2389
                     /label=NheI
     misc_feature    2390..2464
                     /label=K-LOV with Wenjing insertions
     misc_feature    2396..2398
                     /label=L2R
     misc_feature    2426..2428
                     /label=S12N
     misc_feature    2465..2467
                     /label=asLOV2
     misc_feature    2465..2467
                     /label=V fast kinetics
     misc_feature    2468..2827
                     /label=K-LOV with Wenjing insertions
                     /label=K-LOV with Wenjing insertions(1)
     misc_feature    2474..2476
                     /label=Val28Ala
     misc_feature    2780..2782
                     /label=I130V
     misc_feature    2807..2827
                     /label=TEVrs
     misc_feature    2825..2827
                     /label=Met
     misc_feature    2834..2839
                     /label=BamHI
     misc_feature    2840..2863
                     /label=Flag
     misc_feature    2864..3643
                     /label=GAL4
     misc_feature    complement(3647..3649)
                     /label=double stop
     misc_feature    3650..3655
                     /label=EcoRI
     misc_feature    3662..3893
                     /label=PolyA
     misc_feature    3922..4062
                     /label=ITR
                     /label=ITR(1)
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca tagtaatcaa ttacggggtc attagttcat
      181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      301 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
      361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      781 gcttgccacc atggagacag acacactcct gctatgggta ctgctgctct gggttccagg
      841 ttccactggt gacggggccc agccggccag atcttaccca tacgatgtgc cagattacgc
      901 ctatccctat gacgtccctg attacgctct cgacttccag aaggcctcca gcatagtcta
      961 taagaaagag ggggaacagg tggagttctc cttcccactc gcctttacag ttgaaaagct
     1021 gacgggcagt ggcgagctgt ggtggcaggc ggagagggct tcctcctcca agtcttggat
     1081 cacctttgac ctgaagaaca aggaagtgtc tgtaaaacgg gttacccagg accctaagct
     1141 ccagatgggc aagaagctcc cgctccacct caccctgccc caggccttgc ctcagtatgc
     1201 tggctctgga aacctcaccc tggcccttga agcgaaaaca ggaaagttgc atcaggaagt
     1261 gaacctggtg gtgatgagag ccactcagct ccagaaaaat ttgacctgtg aggtgtgggg
     1321 acccacctcc cctaagctga tgctgagctt gaaactggag aacaaggagg caaaggtctc
     1381 gaagcgggag aaggcggtgt gggtgctgaa ccctgaggcg gggatgtggc agtgtctgct
     1441 gagtgactcg ggacaggtcc tgctggaatc caacatcaag gttctgccca catggtccac
     1501 cccggtgcag ccaatggccc tgattgtgct ggggggcgtc gccggcctcc tgcttttcat
     1561 tgggctaggc atcttcttct gtgtcaggtg ccggcaccga aggcgcaagg gctcgggctc
     1621 gacctcgggc tcgggcagcg gtggctctag aggttctggt ggcagctctg gaggtatgaa
     1681 tggagctata ggaggtgacc ttttgctcaa ttttcctgac atgtcggtcc tagagcgcca
     1741 aagggctcac ctcaagtacc tcaatcccac ctttgattct cctctcgccg gcttctttgc
     1801 cgattcttca atgattaccg gcggcgagat ggacagctat ctttcgactg ccggtttgaa
     1861 tcttccgatg atgtacggtg agacgacggt ggaaggtgat tcaagactct caatttcgcc
     1921 ggaaacgacg cttgggactg gaaatttcaa ggcagcgaag tttgatacag agactaagga
     1981 ttgtaatgag gcggcgaaga agatgacgat gaacagagat gacctagtag aagaaggaga
     2041 agaagagaag tcgaaaataa cagagcaaaa caatgggagc acaaaaagca tcaagaagat
     2101 gaaacacaaa gccaagaaag aagagaacaa tttctctaat gattcatcta aagtgacgaa
     2161 ggaattggag aaaacggatt atattcatgg tggctctggt agcttcaacg ctcgcaggaa
     2221 gctggctggt gctatcctgt tcaccatgct cgccacaaga aacttcagcg gcagctttaa
     2281 tgcgcggcgc aagttggccg gagccatact tttcacaatg ttggctacca gaaatttttc
     2341 ggagctggcc gagaagctgg ccggcctgga catcaacggt ggagctagcg agtttcgggc
     2401 aaccacactg gaacggatcg agaaaagctt cgtgattact gatccgagac tgcctgacaa
     2461 cccagtgatt tttgtgagcg attccttcct gcagctgaca gaatattctc gggaagagat
     2521 cctggggcgc aattgccgtt ttctgcaggg acccgagaca gaccgtgcca ctgttcggaa
     2581 aatcagagat gctattgaca accagactga agtgaccgtt cagctgatca attataccaa
     2641 gagcggcaag aagttctgga acgtgttcca cctgcagccg atgcgcgatt ataagggcga
     2701 cgtccagtac ttcattggcg tgcagctgga tggcaccgaa cgtcttcatg gcgccgctga
     2761 gcgtgaggcg gtctgcctgg tcaaaaagac agcctttcag attgctgaga acctgtactt
     2821 ccagatgggc ggaggatccg actacaagga tgacgacgat aagaagctac tgtcttctat
     2881 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
     2941 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
     3001 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
     3061 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
     3121 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
     3181 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
     3241 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
     3301 ggccaatttt aatcaaagtg ggaatattgc tgatagctca ttgtccttca ctttcactaa
     3361 cagtagcaac ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc
     3421 aattgcctcc tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat
     3481 tgatgatggt aataattcaa aaccactgtc acctggttgg acggaccaaa ctgcgtataa
     3541 cgcgtttgga atcactacag ggatgtttaa taccactaca atggatgatg tatataacta
     3601 tctattcgat gatgaagata ccccaccaaa cccaaaaaaa gagtagtaag aattcctcga
     3661 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     3721 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     3781 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     3841 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggaggtaacc
     3901 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     3961 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4021 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4081 ttctccttac gcatctgtgc ggtattcaca ccgcatacgt caaagcaacc atagtacgcg
     4141 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4201 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4261 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4321 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     4381 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4441 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     4501 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4561 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     4621 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4681 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4741 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     4801 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc
     5701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6121 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     6181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
