LOCUS       Exported                6363 bp ds-DNA     circular SYN 30-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 30, 2020 from SnapGene Viewer 5.2.0
            https://www.snapgene.com
COMMENT     Template 155A digested with BamHI, NheI
            f1:gaagcttGCCACCatgCACCTGAGAATCCACGCGAGACGG
            f2:gtcgtgcctgagagcgcagtcgagaagaagcttGCCACCatgCACCTGAGAATC
            r1:GTATGGCTCCGGCcAGCTTGCGCCGCGC
            f2:GCGCGGCGCAAGCTgGCCGGAGCCATAC
            r2:CTTATCGTCGTCATCCTTGTAGTCggatc
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /label=ITR
     misc_feature    151..628
                     /label=synapsin promoter
     misc_feature    649..651
                     /label=Ig k-chain signal sequence
     misc_feature    join(652..810,838..975)
                     /label=NRX3b
                     /label=NRX3b(1)
     misc_feature    811..837
                     /label=HA tag
     misc_feature    976..984
                     /label=NRX3b
     misc_feature    985..996
                     /label=NRX3b
                     /label=NRX3b(2)
     misc_feature    997
                     /label=10 aa linker
                     /label=10 aa linker(2)
     misc_feature    998..1014
                     /label=10 aa linker
                     /label=10 aa linker(1)
     misc_feature    998..1014
                     /label=annealing region for overlap
     misc_feature    1015..1023
                     /label=10 aa linker
     misc_feature    1063..1575
                     /label=CIBN
     misc_feature    1588..1668
                     /label=Nav1.6
     misc_feature    join(1684..1704,1708..1731,1735..1749)
                     /label=M13O (CaMKII)
     misc_feature    1822..1857
                     /label=NNES
     misc_feature    1864..1869
                     /label=nhe1
     misc_feature    1864..1869
                     /label=NheI
     misc_feature    1870..2307
                     /label=K-LOV with Wenjing insertions
     misc_feature    1876..1878
                     /label=L2R
     misc_feature    1906..1908
                     /label=S12N
     misc_feature    1954..1956
                     /label=Val28Ala
     misc_feature    2260..2262
                     /label=I130V
     misc_feature    2287..2307
                     /label=TEVrs
     misc_feature    2305..2307
                     /label=Met
     misc_feature    2314..2319
                     /label=BamHI
     misc_feature    2320..2343
                     /label=Flag
     CDS             2344..3348
                     /gene="tTA"
                     /label=tTA
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
     misc_feature    complement(3349..3351)
                     /label=double stop
     misc_feature    3355..3360
                     /label=EcoRI
     misc_feature    3367..3598
                     /label=PolyA
     misc_feature    3627..3767
                     /label=ITR
                     /label=ITR(1)
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaagaagc ttgccaccat gcacctgaga
      661 atccacgcga gacggagccc tcctcgccgg ccggcctgga cgcttgggat ctggttcctg
      721 ttctggggat gtatcgtcag ctctgtatgg agtcaattgt cttctaatgt agcttcctcc
      781 tcctccacct cttcctcgcc ggggtctcac tacccatacg atgtgccaga ttacgccaac
      841 cccacggagc cgggaatcag acgggttccg ggggcctcag aggtgatccg ggagtcgagc
      901 agcacaacag ggatggtcgt cggcattgtg gctgctgccg ccctctgcat cttgatcctc
      961 ctgtacgcca tgtacaagta caggaacagg gacgagggct cgggctcgac ctcgggctcg
     1021 ggcagcggtg gctctggagg ttctggtggc agctctggag gtatgaatgg agctatagga
     1081 ggtgaccttt tgctcaattt tcctgacatg tcggtcctag agcgccaaag ggctcacctc
     1141 aagtacctca atcccacctt tgattctcct ctcgccggct tctttgccga ttcttcaatg
     1201 attaccggcg gcgagatgga cagctatctt tcgactgccg gtttgaatct tccgatgatg
     1261 tacggtgaga cgacggtgga aggtgattca agactctcaa tttcgccgga aacgacgctt
     1321 gggactggaa atttcaaggc agcgaagttt gatacagaga ctaaggattg taatgaggcg
     1381 gcgaagaaga tgacgatgaa cagagatgac ctagtagaag aaggagaaga agagaagtcg
     1441 aaaataacag agcaaaacaa tgggagcaca aaaagcatca agaagatgaa acacaaagcc
     1501 aagaaagaag agaacaattt ctctaatgat tcatctaaag tgacgaagga attggagaaa
     1561 acggattata ttcatagcgg tagtggcacc gtgcgcgtgc cgattgcggt gggcgaaagc
     1621 gattttgaaa acctgaacac cgaagatgtg agcagcgaaa gcgatccggg tggctctggt
     1681 agcttcaacg ctcgcaggaa gctggctggt gctatcctgt tcaccatgct cgccacaaga
     1741 aacttcagcg gcagctttaa tgcgcggcgc aagctggccg gagccatact tttcacaatg
     1801 ttggctacca gaaatttttc ggagctggcc gagaagctgg ccggcctgga catcaacggt
     1861 ggagctagcg agtttcgggc aaccacactg gaacggatcg agaaaagctt cgtgattact
     1921 gatccgagac tgcctgacaa cccaatcatt tttgtgagcg attccttcct gcagctgaca
     1981 gaatattctc gggaagagat cctggggcgc aattgccgtt ttctgcaggg acccgagaca
     2041 gaccgtgcca ctgttcggaa aatcagagat gctattgaca accagactga agtgaccgtt
     2101 cagctgatca attataccaa gagcggcaag aagttctgga acgtgttcca cctgcagccg
     2161 atgcgcgatt ataagggcga cgtccagtac ttcattggcg tgcagctgga tggcaccgaa
     2221 cgtcttcatg gcgccgctga gcgtgaggcg gtctgcctgg tcaaaaagac agcctttcag
     2281 attgctgaga acctgtactt ccagatgggc ggaggatccg actacaagga tgacgacgat
     2341 aagatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
     2401 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
     2461 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
     2521 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
     2581 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
     2641 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
     2701 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
     2761 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
     2821 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
     2881 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
     2941 caacttaaat gtgaaagtgg gtccgcgtac agccgcgcgc gtacgaaaaa caattacggg
     3001 tctaccatcg agggcctgct cgatctcccg gacgacgacg cccccgaaga ggcggggctg
     3061 gcggctccgc gcctgtcctt tctccccgcg ggacacacgc gcagactgtc gacggccccc
     3121 ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg
     3181 catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt
     3241 ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag
     3301 tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtgggta ataggaattc
     3361 ctcgagctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3421 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3481 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3541 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggagg
     3601 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     3661 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     3721 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     3781 gtattttctc cttacgcatc tgtgcggtat tcacaccgca tacgtcaaag caaccatagt
     3841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4021 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     4081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4141 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     4201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4261 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     4321 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     4501 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4561 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4621 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggaa
     5401 gccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5521 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5581 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5821 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6361 tgt
//
