LOCUS       Exported                6764 bp ds-DNA     circular SYN 30-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 30, 2020 from SnapGene Viewer 5.2.0
            https://www.snapgene.com
COMMENT     Vector CMS375 digested with NcoI and EcoRI
            template CMS375
            f1:gagagcgcagtcgagaaGGATCCgccaccatg
            r1:GGCGTAATCCACATGGCTTCCGTAAGATC
            band 1300bp
            Template CMS466
            f1:GATCTTACGGAAGCCATGTGGATTACGCCGGAGAAtccttgtttaagggaccacgtg
            r1:gattatcgataagcttgatatcGAATTCTTAcaccatgaaaactttatggcccccccac
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /label=ITR
                     /label=ITR(1)
     misc_feature    151..628
                     /label=synapsin promoter
     misc_feature    642..647
                     /label=Kozak consensus
     misc_feature    648..1364
                     /label=eGFP
     misc_feature    1401..1403
                     /label=start codon
     misc_feature    join(1404..1454,1458..1502,1506..1844)
                     /label=CaM from GCaMP5
     misc_feature    1455..1457
                     /label=F19L
     misc_feature    1503..1505
                     /label=V35G
     misc_feature    1845..1886
                     /label=V5
     misc_feature    1887..1916
                     /label=GGSGSGGSGSGSGGS linker
     misc_feature    1917..2426
                     /label=Nanoluc
     misc_feature    2427..2471
                     /label=15 aa linker
     misc_feature    join(2472..3106,3108..3128)
                     /label=TEV full length 242
     misc_feature    2928..2930
                     /label=Ser153Asn
     misc_feature    3107
                     /label=Silent mutation
     misc_feature    3126..3128
                     /label=S 219
     misc_feature    3132..3137
                     /label=EcoRI
     misc_feature    3146..3754
                     /label=WPRE
     misc_feature    3768..3999
                     /label=PolyA
     misc_feature    4028..4168
                     /label=ITR
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cgccaccatg gtgagcaagg
      661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      781 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      841 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1081 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1141 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1201 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1261 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1321 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggacctt ggatctggca
     1381 caggcggatc cggttcaggc atggaccaac tgactgaaga gcagatcgca gaatttaaag
     1441 aggctttctc cctactggac aaggacgggg atgggacaat aacaaccaag gagctgggga
     1501 cggggatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac atgatcaatg
     1561 aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca atgatggcaa
     1621 gaaaaatgaa atacacagac agtgaagaag aaattagaga agcgttccgt gtgtttgata
     1681 aggatggcaa tggctacatc agtgcagcag agcttcgcca cgtgatgaca aaccttggag
     1741 agaagttaac agatgaagag gttgatgaaa tgatcaggga agcagacatc gatggggatg
     1801 gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc gaagggcaag ccgatcccta
     1861 accctctgct gggcctggac agcactggtg gaagtggatc aggcagcggt ggatctgtct
     1921 tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac ctggaccaag
     1981 tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc gtaactccga
     2041 tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat gtcatcatcc
     2101 cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt aaggtggtgt
     2161 accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg gtaatcgacg
     2221 gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc gccgtgttcg
     2281 acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt atcgacgagc
     2341 gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga gtgaccggct
     2401 ggcggctgtg cgaacgcatt ctggcgggcg acccactagt ccagtgtggt ggaattgccg
     2461 gatccgccac cggagaatcc ttgtttaagg gaccacgtga ttacaacccg atatcgagca
     2521 ccatttgtca tttgacgaat gaatctgatg ggcacacaac atcgttgtat ggtattggat
     2581 ttggtccctt catcattaca aacaagcact tgtttagaag aaataatgga acactgttgg
     2641 tccaatcact acatggtgta ttcaaggtca agaacaccac gactttgcaa caacacctca
     2701 ttgatgggag ggacatgata attattcgca tgcctaagga tttcccacca tttcctcaaa
     2761 agctgaaatt tagagagcca caaagggaag agcgcatatg tcttgtgaca accaacttcc
     2821 aaactaagag catgtctagc atggtgtcag acactagttg cacattccct tcatctgatg
     2881 gcatattctg gaagcattgg attcaaacca aggatgggca gtgtggcaat ccattagtat
     2941 caactagaga tgggttcatt gttggtatac actcagcatc gaatttcacc aacacaaaca
     3001 attatttcac aagcgtgccg aaaaacttca tggaattgtt gacaaatcag gaggcgcagc
     3061 agtgggttag tggttggcga ttaaatgctg actcagtatt gtgggggggc cataaagttt
     3121 tcatggtgta agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     3181 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3241 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3301 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3361 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3421 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3481 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3541 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     3601 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3661 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3721 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagcta gagctcgctg
     3781 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3841 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3901 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3961 gggggaggat tgggaagaca atagcaggca tgctggggag gtaaccacgt gcggaccgag
     4021 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4081 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4141 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4201 ctgtgcggta ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4261 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4321 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4381 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4441 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4501 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4561 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     4621 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4681 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4741 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4801 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4861 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4921 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4981 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5041 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5101 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5161 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5221 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5281 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5341 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5401 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5461 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5521 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5581 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5641 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5701 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5761 ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc
     5821 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5881 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5941 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6001 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6061 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6121 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6181 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6241 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6301 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6361 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6421 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6481 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6541 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6601 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6661 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6721 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
