LOCUS       Exported                7300 bp ds-DNA    circular SYN 28-Ê®ÔÂ-2020
DEFINITION  Prokaryotic expression vector / N-Term (GST-His) Tag + C-Term (His) 
            Tag / Enterokinase cleavage.
ACCESSION   .
VERSION     .
KEYWORDS    (V12) P.c.AAD1155 - pGS-21a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2020Äê10ÔÂ28ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..17
                     /label="T7 Promoter"
                     /note="T7 Promoter"
     misc_feature    20..44
                     /label="lac operator"
                     /note="lac operator"
     misc_feature    101..118
                     /label="His-Tag"
                     /note="His-Tag"
     misc_feature    128..787
                     /label="Gst-Tag"
                     /note="Gst-Tag"
     misc_feature    818..832
                     /label="Enterokinase cleavage"
                     /note="Enterokinase cleavage"
                     /note="Enterokinase recognition/cleavage region"
     CDS             854..2008
                     /codon_start=1
                     /label="P.c. AAD"
                     /note="P.c. AAD"
                     /translation="MNIWAPAPEPPTKLGRHRQLAPGCGLHVSPIQLGAMSIGDKWHPY
                     GMGTMDKEASFKLLDAFYNAGGNFIDTANVYQDETSEEFIGEWMEARGNRDQMVVATKY
                     SLVYKRGASFEEIPQKTQYVGNSLKSMHISVHDSLRKLRTSYIDIFYVHFWDYTCTIEE
                     VMNGLHNLVAQGKVLYLGVSDTPAWVVSKANNYARMAGKTPFVIYEGEWNITMRDMERD
                     IIPMCIHEGMAIAPWNVLCAGKIRTDAEEERRLKSGEGGRTLLQFDGWLRNETERKVSK
                     ALEKVAEEIGAKSITSVAIAYLMQKFPYVFPIVGGRKVEHLYANLEALDISLSPEQMQF
                     LNDTVPFNKGFPYLLFGDGSDYNIVHKAAGHYDKWPAQQAIRPQK"
     misc_feature    2024..2041
                     /label="His-Tag"
                     /note="His-Tag"
     terminator      2108..2155
                     /label="T7 Terminator"
                     /note="T7 Terminator"
                     /note="T7 Terminator (does it start at ctagcat or at tagcat
                     ?)"
     misc_feature    2192..2647
                     /label="F1 ori"
                     /note="F1 ori"
                     /note="f1 ori (I suppose this is the origin for production 
                     of virions containing single-stranded DNA that corresponds 
                     to the coding strand after infection with helper phage)"
     CDS             2779..3639
                     /codon_start=1
                     /label="Amp(R)"
                     /note="Amp"
                     /note="Beta-lactamase gene region (ampicillin resistance 
                     gene)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gtctggttct catcatcatc atcatcatag
      121 cagcggtatg tcccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg
      181 acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg
      241 tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta
      301 tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa
      361 gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc
      421 ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct
      481 caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg
      541 tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc
      601 tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg
      661 ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta
      721 tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa
      781 atcggatctg ggccacacag gccatagatc tggtaccgac gacgacgaca aggccatggc
      841 tgatatcgga tccatgaaca tctgggcacc cgcacctgag cctccgacta agctcggccg
      901 tcacaggcag cttgctcccg gatgtggtct ccatgtatcg cccatccagc tcggcgcaat
      961 gagtatcggc gacaagtggc atccatatgg catgggcacc atggacaagg aagccagctt
     1021 caagctcctc gacgcattct acaacgctgg tggtaacttc attgataccg ctaatgtcta
     1081 ccaagacgag acatccgagg aatttattgg cgagtggatg gaggctcggg gcaaccgcga
     1141 ccagatggtc gttgctacca agtacagtct ggtctacaaa cggggtgcca gcttcgaaga
     1201 gatcccgcag aagacgcagt atgtcggcaa cagcctcaag tcgatgcaca tctcagtgca
     1261 cgactcattg cgcaagctgc gcacgagcta catcgatata ttctacgttc acttctggga
     1321 ctacacttgc acaattgagg aggtgatgaa cggcttgcac aatcttgttg cacagggcaa
     1381 ggttctctac ctcggtgtct cggacacacc cgcctgggtt gtctcgaagg cgaacaacta
     1441 tgcgcgcatg gccggcaaga cgcctttcgt catctacgag ggagagtgga acatcacgat
     1501 gcgcgacatg gagcgcgaca tcattccgat gtgcattcac gaagggatgg ccatcgcgcc
     1561 ctggaatgtg ctttgcgccg gcaagatacg cacggacgcc gaggaggagc gccgtctcaa
     1621 gtccggcgag ggtgggcgta cgctcctaca gttcgacgga tggctccgga acgagaccga
     1681 gcgcaaggtc agcaaggcac tcgagaaggt cgctgaagag atcggcgcaa agagtatcac
     1741 atccgtcgca attgcgtacc tcatgcagaa gttcccctac gtcttcccca ttgtcggtgg
     1801 acgcaaggtc gaacacctgt acgccaacct cgaggcgctc gacatctcgc tcagccccga
     1861 gcagatgcag ttcctgaacg acacagtacc cttcaacaag ggcttcccat accttctctt
     1921 cggtgatggg tccgactaca acatcgtaca caaggctgca ggtcattatg acaagtggcc
     1981 cgcgcaacag gctatccgcc cccagaaggc ggccgcactc gagcaccacc accaccacca
     2041 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     2101 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     2161 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     2221 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2281 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2341 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2401 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2461 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2521 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2581 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     2641 acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2701 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2761 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2821 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2881 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2941 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3001 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3061 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3121 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3181 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3241 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3301 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     3361 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3421 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3481 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3541 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3601 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3661 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3721 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3781 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3841 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3901 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3961 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4021 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4081 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4141 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4201 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4261 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4321 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4381 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4441 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4501 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4561 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4621 atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4681 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     4741 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4801 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4861 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     4921 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     4981 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     5041 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     5101 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     5161 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     5221 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     5281 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     5341 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     5401 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     5461 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
     5521 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     5581 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     5641 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     5701 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     5761 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     5821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     5941 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     6001 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     6061 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagatg tccgcaccaa
     6121 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     6181 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     6241 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     6301 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     6361 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     6421 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     6481 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     6541 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     6601 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     6661 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     6721 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     6781 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     6841 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     6901 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     6961 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     7021 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     7081 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     7141 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     7201 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     7261 ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat
//
