LOCUS       AF097411                5037 bp    DNA     circular SYN 28-JAN-2002
DEFINITION  Expression vector pGST-parallel1, complete sequence.
ACCESSION   AF097411
VERSION     AF097411.1  GI:3983116
KEYWORDS    .
SOURCE      Expression vector pGST-parallel1
  ORGANISM  Expression vector pGST-parallel1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Sheffield,P., Garrard,S. and Derewenda,Z.
  TITLE     Overcoming expression and purification problems of RhoGDI using a
            family of 'parallel' expression vectors
  JOURNAL   Protein Expr. Purif. 15 (1), 34-39 (1999)
   PUBMED   10024467
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Sheffield,P.J., Garrard,S.M. and Derewenda,Z.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-1998) Molecular Physiology and Biological
            Physics, University of Virginia, 4215 Jordan Hall, 1300 Jefferson
            Park Avenue, Charlottesville, Virginia 22908, USA
FEATURES             Location/Qualifiers
     source          join(1..957,2223..6302)
                     /dnas_title="pGST-Fah_wt"
                     /organism="Expression vector pGST-parallel1"
                     /mol_type="other DNA"
                     /db_xref="taxon:84478"
     regulatory      183..211
                     /regulatory_class="promoter"
                     /note="tac; inducible promoter"
     CDS             join(258..957,2223..2314)
                     /dnas_title="glutathione S-transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="AAC83809.1"
                     /db_xref="GI:3983117"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     IDTTENLYFQGAMDPEFKGLRRRAQLVRPLSNLEPAVSSGRIVTD"
     misc_feature    924..944
                     /note="Tobacco etch virus protease cleavage site"
     misc_feature    join(946..957,2223..2299)
                     /note="multiple cloning site"
     gene            2640..3570
                     /dnas_title="bla"
                     /gene="bla"
     regulatory      2640..2668
                     /dnas_title="bla"
                     /gene="bla"
                     /regulatory_class="promoter"
     CDS             2710..3570
                     /dnas_title="bla"
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAC83810.1"
                     /db_xref="GI:3983118"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            4774..5733
                     /dnas_title="lacIq"
                     /gene="lacIq"
     CDS             4774..5733
                     /dnas_title="lacIq"
                     /gene="lacIq"
                     /codon_start=1
                     /transl_table=11
                     /product="lac repressor"
                     /protein_id="AAC83811.1"
                     /db_xref="GI:3983119"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"
     vector          join(1..957,2223..6302)
                     /source="pGST-Parallel1_AF097411"
                     /type="Custom cloned vector"
     misc_feature    947..961
                     /note="EcoRI15L"
     misc_feature    2223..2237
                     /note="EcoRI15R"
     vector          join(1..961,2223..6302)
                     /source="pGST-EcoRI"
                     /type="Custom cloned vector"
     insert          963..2222
                     /source="Fah_NM_010176"
                     /type="Custom cloned insert"
     CDS             963..2222
                     /dnas_title="Fah"
                     /gene="Fah"
                     /EC_number="3.7.1.2"
                     /note="beta-diketonase; FAA"
                     /codon_start=1
                     /product="fumarylacetoacetase"
                     /protein_id="NP_034306.2"
                     /db_xref="GI:240120112"
                     /db_xref="CCDS:CCDS21418.1"
                     /db_xref="GeneID:14085"
                     /db_xref="MGI:MGI:95482"
                     /translation="MSFIPVAEDSDFPIQNLPYGVFSTQSNPKPRIGVAIGDQILDLS
                     VIKHLFTGPALSKHQHVFDETTLNNFMGLGQAAWKEARASLQNLLSASQARLRDDKEL
                     RQRAFTSQASATMHLPATIGDYTDFYSSRQHATNVGIMFRGKENALLPNWLHLPVGYH
                     GRASSIVVSGTPIRRPMGQMRPDNSKPPVYGACRLLDMELEMAFFVGPGNRFGEPIPI
                     SKAHEHIFGMVLMNDWSARDIQQWEYVPLGPFLGKSFGTTISPWVVPMDALMPFVVPN
                     PKQDPKPLPYLCHSQPYTFDINLSVSLKGEGMSQAATICRSNFKHMYWTMLQQLTHHS
                     VNGCNLRPGDLLASGTISGSDPESFGSMLELSWKGTKAIDVEQGQTRTFLLDGDEVII
                     TGHCQGDGYRVGFGQCAGKVLPALSPA"
     exon            1044..1154
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1155..1276
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1165..1165
                     /note="A>G; swingshift; N68S"
     misc_feature    962..983
                     /note="62.8c"
     exon            1277..1326
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1327..1417
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1418..1515
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1516..1568
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1569..1668
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1669..1799
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1800..1875
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1876..1922
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            1923..2024
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            2025..2142
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2203..2219
                     /note="60.4c"
     misc_feature    2205..2222
                     /note="62.36c"
     exon            963..1043
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     exon            2143..2222
                     /dnas_title="Fah"
                     /gene="Fah"
                     /inference="alignment:Splign:1.39.8"
     PCR_primer      947..983
                     /note="EcFahF 62.8c"
     PCR_primer      complement(2205..2237)
                     /note="EcFahR 62.4c"
     PCR_primer      872..888
                     /note="GSTf 60c"
     PCR_primer      complement(2295..2312)
                     /note="GSTr 61c"
     PCR_primer      complement(1213..1237)
                     /note="SR"
     PCR_primer      complement(1815..1833)
                     /note="FahR1 59.2c"
ORIGIN      
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa aatcgatacg accgaaaacc tgtattttca gggcgccatg gatccggaat
      961 tcatgtcctt tattccagtg gccgaggact ccgactttcc catccaaaac ctgccctatg
     1021 gtgttttctc cactcaaagc aacccaaagc cacggattgg tgtagccatc ggtgaccaga
     1081 tcttggacct gagtgtcatt aaacacctct ttaccggacc tgccctttcc aaacatcaac
     1141 atgtcttcga tgagacaact ctcaataact tcatgggtct gggtcaagct gcatggaagg
     1201 aggcaagagc atccttacag aacttactgt ctgccagcca agcccggctc agagatgaca
     1261 aggagcttcg gcagcgtgca ttcacctccc aggcttctgc gacaatgcac cttcctgcta
     1321 ccataggaga ctacacggac ttctactctt ctcggcagca tgccaccaat gttggcatta
     1381 tgttcagagg caaggagaat gcgctgttgc caaattggct ccacttacct gtgggatacc
     1441 atggccgagc ttcctccatt gtggtatctg gaaccccgat tcgaagaccc atggggcaga
     1501 tgagacctga taactcaaag cctcctgtgt atggtgcctg cagactctta gacatggagt
     1561 tggaaatggc tttcttcgta ggccctggga acagattcgg agagccaatc cccatttcca
     1621 aagcccatga acacattttc gggatggtcc tcatgaacga ctggagcgca cgagacatcc
     1681 agcaatggga gtacgtccca cttgggccat tcctggggaa aagctttgga accacaatct
     1741 ccccgtgggt ggtgcctatg gatgccctca tgccctttgt ggtgccaaac ccaaagcagg
     1801 accccaagcc cttgccatat ctctgccaca gccagcccta cacatttgat atcaacctgt
     1861 ctgtctcttt gaaaggagaa ggaatgagcc aggcggctac catctgcagg tctaacttta
     1921 agcacatgta ctggaccatg ctgcagcaac tcacacacca ctctgttaat ggatgcaacc
     1981 tgagacctgg ggacctcttg gcttctggaa ccatcagtgg atcagaccct gaaagctttg
     2041 gctccatgct ggaactgtcc tggaagggaa caaaggccat cgatgtggag caggggcaga
     2101 ccaggacctt cctgctggac ggcgatgaag tcatcataac aggtcactgc cagggggacg
     2161 gctaccgtgt tggctttggc cagtgtgctg ggaaagtgct gcctgccctt tcaccagcct
     2221 gaaattcaaa ggcctacgtc gacgagctca actagtgcgg ccgctttcga atctagagcc
     2281 tgcagtctcg agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat
     2341 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     2401 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     2461 gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc
     2521 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2581 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2641 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2701 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2761 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2821 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2881 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     2941 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3001 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3061 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3121 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3181 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3241 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3301 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     3361 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3421 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3481 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     3541 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     3601 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3661 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3721 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3781 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3841 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3901 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3961 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4021 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4081 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4141 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4201 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4261 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4321 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4381 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4441 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4501 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4561 cgcataaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc
     4621 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca
     4681 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
     4741 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
     4801 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
     4861 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
     4921 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
     4981 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
     5041 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
     5101 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
     5161 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
     5221 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
     5281 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
     5341 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
     5401 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
     5461 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
     5521 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     5581 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     5641 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     5701 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     5761 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     5821 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
     5881 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5941 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6001 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
     6061 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
     6121 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta
     6181 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
     6241 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa
     6301 tt
//

