LOCUS       Exported                6224 bp ds-DNA     circular SYN 02-NOV-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Rashid, F.Z.M.
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 2, 2020 from SnapGene Viewer 5.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; 
                     thearaC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             1329..2024
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein 
                     evolvedfrom a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     CDS             2025..2201
                     /codon_start=1
                     /label=H-NSdbd
                     /translation="AAVKSGTKAKRAQRPAKYSYVDENGETKTWTGQGRTPAVIKKAMD
                     EQGKSLDDFLIKQ"
     terminator      2437..2523
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli 
                     rrnBgene"
     terminator      2615..2642
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli 
                     rrnBgene"
     promoter        2661..2752
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3206..3661
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; 
                     arrowindicates direction of (+) strand synthesis"
     CDS             complement(4216..4875)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4876..4978)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene 
                     encodingchloramphenicol acetyltransferase"
     rep_origin      complement(5504..6049)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number 
                     p15Aorigin of replication can be propagated in E. coli 
                     cells that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcagg
     1321 agtgtgaaat ggtgagcaag ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc
     1381 acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1441 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct
     1501 cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcatc aagcaccccg
     1561 ccgacatccc cgactactat aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga
     1621 tgaacttcga ggacggcggc gccgtgaccg tgacccagga cacctccctg gaggacggca
     1681 ccctgatcta caaggtgaag ctccgcggca ccaacttccc tcctgacggc cccgtaatgc
     1741 agaagaagac aatgggctgg gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc
     1801 tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac ctggcggact
     1861 tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc
     1921 gcaagttgga catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct
     1981 ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggctgcc gttaaatctg
     2041 gcaccaaagc taaacgtgct cagcgtccgg caaaatatag ctacgttgac gaaaacggcg
     2101 aaactaaaac ctggactggc caaggccgta ctccagctgt aatcaaaaaa gcaatggatg
     2161 agcaaggtaa atccctcgac gatttcctga tcaagcaata aagctctaga gtcgacctgc
     2221 aggcatgcaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     2281 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
     2341 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
     2401 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     2461 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     2521 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     2581 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     2641 tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct
     2701 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2761 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2821 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttggggc aaactattaa
     2881 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2941 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3001 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3061 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3121 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3181 actcatatat actttagatt gatttacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3241 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3301 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3361 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3421 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3481 ttggagtcca cgttctttaa tagtggactc ttgttccaaa cttgaacaac actcaaccct
     3541 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3601 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     3661 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3721 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3901 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtcaggc atttgagaag
     4021 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
     4081 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
     4141 cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata
     4201 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     4261 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
     4321 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     4381 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     4441 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     4501 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     4561 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     4621 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     4681 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     4741 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     4801 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     4861 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     4921 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     4981 gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt
     5041 tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg
     5101 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
     5161 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
     5221 gcaccgccgg acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga
     5281 gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag
     5341 aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     5401 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     5461 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     5521 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     5581 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     5641 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     5701 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     5761 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     5821 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     5881 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     5941 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     6001 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     6061 tattaatcag ataaaatatt tgctcatgag cccgaagtgg cgagcccgat cttccccatc
     6121 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     6181 gatgcgtccg gcgtagagga tctgctcatg tttgacagct tatc
//
