LOCUS       pLyGo-BS-3      6601 bp    DNA     circular UNA 05-NOV-2020
DEFINITION  .
KEYWORDS    "Responsible person/group" "Usage" "Status" "Parent plasmids"      
            "Cloning sites" "Antibiotic resistance" "Origin of replication"
            "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers" "gRNAs"
            "Terminators" "DNA fragments" "Backbones" "Design entry"
            "LabCollector ID" "Comment"
FEATURES             Location/Qualifiers
     rep_origin      4..459
                     /label="f1 ori"
     CDS             complement(552..1367)
                     /label="KanR"
     rep_origin      1489..2077
                     /label="ori"
     misc_feature    2263..2405
                     /label="bom"
     CDS             complement(2507..2698)
                     /label="rop"
     misc_feature    2807..3329
                     /label="amyE upstream"
     misc_feature    complement(3404..4054)
                     /label="camR"
     misc_feature    4202..4306
                     /label="T1 rrnB"
     misc_feature    4480..5269
                     /label="P3P"
     RBS             5168..5179
                     /label="Strong RBS"
     RBS             5270..5293
                     /label="R0"
     misc_feature    5294..5377
                     /label="aprE"
                     /label="AprESP"
     misc_feature    5393..5426
                     /label="BBa_B1002"
     misc_feature    5427..5529
                     /label="PccdB"
     CDS             5530..5835
                     /label="ccdB"
     misc_feature    5704
                     /label="ccdB(2)"
     misc_feature    5875..5977
                     /label="T0 lambda"
     misc_feature    5998..6567
                     /label="amyE downstream"
ORIGIN
        1 GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
       61 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
      121 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
      181 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
      241 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
      301 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT
      361 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
      421 TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTC AGGTGGCACT TTTCGGGGAA
      481 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
      541 TGAATTAATT CTTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTT ATTCATATCA
      601 GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG
      661 AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA
      721 TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA
      781 TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGTTTAT GCATTTCTTT CCAGACTTGT
      841 TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAA ACCGTTATTC
      901 ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC TGTTAAAAGG ACAATTACAA
      961 ACAGGAATCG AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT
     1021 GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CGGGGATCGC AGTGGTGAGT
     1081 AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC
     1141 GTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA
     1201 TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT
     1261 GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA
     1321 TTTAATCGCG GCCTAGAGCA AGACGTTTCC CGTTGAATAT GGCTCATAAC ACCCCTTGTA
     1381 TTACTGTTTA TGTAAGCAGA CAGTTTTATT GTTCATGACC AAAATCCCTT AACGTGAGTT
     1441 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     1501 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     1561 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     1621 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     1681 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     1741 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     1801 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     1861 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     1921 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     1981 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     2041 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     2101 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
     2161 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC
     2221 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG GGCCTGATGC GGTATTTTCT
     2281 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC
     2341 TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG
     2401 CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG
     2461 CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC
     2521 CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG
     2581 ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA
     2641 ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG
     2701 ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA
     2761 GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCACT GGCGGTATGT TTGCAAAACG
     2821 ATTCAAAACC TCTTTACTGC CGTTATTCGC TGGATTTTTA TTGCTGTTTC ATTTGGTTCT
     2881 GGCAGGACCG GCGGCTGCGA GTGCTGAAAC GGCGAACAAA TCGAATGAGC TTACAGCACC
     2941 GTCGATCAAA AGCGGAACCA TTCTTCATGC ATGGAATTGG TCGTTCAATA CGTTAAAACA
     3001 CAATATGAAG GATATTCATG ATGCAGGATA TACAGCCATT CAGACATCTC CGATTAACCA
     3061 AGTAAAGGAA GGGAATCAAG GAGATAAAAG CATGTCGAAC TGGTACTGGC TGTATCAGCC
     3121 GACATCGTAT CAAATTGGCA ACCGTTACTT AGGTACTGAA CAAGAATTTA AAGAAATGTG
     3181 TGCAGCCGCT GAAGAATATG GCATAAAGGT CATTGTTGAC GCGGTCATCA ATCATACCAC
     3241 CAGTGATTAT GCCGCGATTT CCAATGAGGT TAAGAGTATT CCAAACTGGA CACATGGAAA
     3301 CACACAAATT AAAAACTGGT CTGATCGATC GTCTCAGCGA GGCGCGTCAG GTTAGTGACA
     3361 TTAGAAAACC GACTGTAAAA AGTACAGTCG GCATTATCTC ATATTATAAA AGCCAGTCAT
     3421 TAGGCCTATC TGACAATTCC TGAATAGAGT TCATAAACAA TCCTGCATGA TAACCATCAC
     3481 AAACAGAATG ATGTACCTGT AAAGATAGCG GTAAATATAT TGAATTACCT TTATTAATGA
     3541 ATTTTCCTGC TGTAATAATG GGTAGAAGGT AATTACTATT ATTATTGATA TTTAAGTTAA
     3601 ACCCAGTAAA TGAAGTCCAT GGAATAATAG AAAGAGAAAA AGCATTTTCA GGTATAGGTG
     3661 TTTTGGGAAA CAATTTCCCC GAACCATTAT ATTTCTCTAC ATCAGAAAGG TATAAATCAT
     3721 AAAACTCTTT GAAGTCATTC TTTACAGGAG TCCAAATACC AGAGAATGTT TTAGATACAC
     3781 CATCAAAAAT TGTATAAAGT GGCTCTAACT TATCCCAATA ACCTAACTCT CCGTCGCTAT
     3841 TGTAACCAGT TCTAAAAGCT GTATTTGAGT TTATCACCCT TGTCACTAAG AAAATAAATG
     3901 CAGGGTAAAA TTTATATCCT TCTTGTTTTA TGTTTCGGTA TAAAACACTA ATATCAATTT
     3961 CTGTGGTTAT ACTAAAAGTC GTTTGTTGGT TCAAATAATG ATTAAATATC TCTTTTCTCT
     4021 TCCAATTGTC TAAATCAATT TTATTAAAGT TCATTTGATA TGCCTCCTAA ATTTTTATCT
     4081 AAAGTGAATT TAGGAGGCTT ACTTGTCTGC TTTCTTCATT AGAATCAATC CTTTTTTAAA
     4141 AGTCAATATT ACTGTAACAT AAATATATAT TTTAAAAATA TCCCACTTAC GCGCCCAGCT
     4201 GTCTAGGGCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA CCGACAAACA ACAGATAAAA
     4261 CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT CGTTTTATTT GATGCCTTTA ATTAAGAAGC
     4321 GGAGCGCGAA TAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG
     4381 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA
     4441 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCGTCTATT CTTTTGGAAG AAAATATAGG
     4501 GAAAATGGTA TTTGTTAAAA ATTCTGAATA TTTATACAAT ATCATATGTT TCACATTGAA
     4561 ATATGCTGTC CAGACTGTCC GCTGTGTAAA AAATAGGAAT AAAGGGGGGT TGTTATTATT
     4621 TTACTGATAT GTAAAATATA ATTTGTATAA GAAAATTCGA AACGTAAGAT GAAACCTTAG
     4681 ATAAAAGTGC TTTTTTTGTT GCAATTGAAG AATTATTAAT GTTAAGCTTA ATTAAAGATA
     4741 ATATCTTTGA ATTGTAACGC CCCTCAAAAG TAAGAACTAC AAAAAAAGAA TACGTTATAT
     4801 AGAAATATGT TTGAACCTTC TTCAGATTAC AAATATATTC GGACGGACTC TACCTCAAAT
     4861 GCTTATCTAA CTATAGAATG ACATACAAGC ACAACCTTGA AAATTTGAAA ATATAACTAC
     4921 CAATGAACTT GTTCATGTGA ATTATCGCTG TATTTAATTT TCTCAATTCA ATATATAATA
     4981 TGCCAATACA TTGTTACAAG TAGAAATTAA GACACCCTTG ATAGCCTTAC TATACCTAAC
     5041 ATGATGTAGT ATTAAATGAA TATGTAAATA TATTTATGAT AAGAAGCGAC TTATTTATAA
     5101 TCATTACATA TTTTTCTATT GGAATGATTA AGATTCCAAT AGAATAGTGT ATAAATTATT
     5161 TATCTTGAAA GGAGGGATGC CTAAAAACGA AGAACATTAA AAACATATAT TTGCACCGTC
     5221 TAATGGATTT ATGAAAAATC ATTTTATCAG TTTGAAAATT ATGTATTATG ATTAACTAAT
     5281 AAGGAGGACA AACATGAAGA AACCGTTGGG GAAAATTGTC GCAAGCACCG CACTACTCAT
     5341 TTCTGTTGCT TTTAGTTCAT CGATCGCATC GGCTGCTCAT CGAAGAGCTT AACGCAAAAA
     5401 ACCCCGCTTC GGCGGGGTTT TTTCGCTACT AAAAGCCAGA TAACAGTATG CGTATTTGCG
     5461 CGCTGATTTT TGCGGTATAA GAATATATAC TGATATGTAT ACCCGAAGTA TGTCAAAAAG
     5521 AGGTATGCTA TGCAGTTTAA GGTTTACACC TATAAAAGAG AGAGCCGTTA TCGTCTGTTT
     5581 GTGGATGTAC AGAGTGATAT TATTGACACG CCCGGGCGAC GGATGGTGAT CCCCCTGGCC
     5641 AGTGCACGTC TGCTGTCAGA TAAAGTCTCC CGTGAACTTT ACCCGGTGGT GCATATCGGG
     5701 GATAAAAGCT GGCGCATGAT GACCACCGAT ATGGCCAGTG TGCCGGTCTC CGTTATCGGG
     5761 GAAGAAGTGG CTGATCTCAG CCACCGCGAA AATGACATCA AAAACGCCAT TAACCTGATG
     5821 TTCTGGGGAA TATAAGCTCT TCGTAAGATC CGGCTGTTCA GCGTCTGAAC TAGTCTTGGA
     5881 CTCCTGTTGA TAGATCCAGT AATGACCTCA GAACTCCATC TGGATTTGTT CAGAACGCTC
     5941 GGTTGCCGCC GGGCGTTTTT TATTGGTGAG AATCCAGGGG TCCCCAATAA TTACGATGCG
     6001 CCTCATGTTT TCCTTGAGAA TTACAAAACA GGTGTAACAC ATTCTTTCAA TGATCAACTG
     6061 ACGATTACCT TGCGTGCAGA TGCGAATACA ACAAAAGCCG TTTATCAAAT CAATAATGGA
     6121 CCAGAGACGG CGTTTAAGGA TGGAGATCAA TTCACAATCG GAAAAGGAGA TCCATTTGGC
     6181 AAAACATACA CCATCATGTT AAAAGGAACG AACAGTGATG GTGTAACGAG GACCGAGAAA
     6241 TACAGTTTTG TTAAAAGAGA TCCAGCGTCG GCCAAAACCA TCGGCTATCA AAATCCGAAT
     6301 CATTGGAGCC AGGTAAATGC TTATATCTAT AAACATGATG GGAGCCGAGT AATTGAATTG
     6361 ACCGGATCTT GGCCTGGAAA ACCAATGACT AAAAATGCAG ACGGAATTTA CACGCTGACG
     6421 CTGCCTGCGG ACACGGATAC AACCAACGCA AAAGTGATTT TTAATAATGG CAGCGCCCAA
     6481 GTGCCCGGTC AGAATCAGCC TGGCTTTGAT TACGTGCTAA ATGGTTTATA TAATGACTCG
     6541 GGCTTAAGCG GTTCTCTTCC CCATTGAAGA AGACTTAGGA ACTATATCCG GATTGGCGAA
     6601 T
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