LOCUS       Exported                5640 bp ds-DNA     circular SYN 09-NOV-2020
DEFINITION  #S261 pMY-CD90.2-Cd247AmiR.
ACCESSION   .
VERSION     .
KEYWORDS    #S261 pMY-CD90.2-Cd247AmiR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 9, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
COMMENT     Vector molecule:  S238 Mlu SphI lin
               Fragment ends: SphI and MluI
               Fragment size: 5135
            Insert molecule:  CD90.2 insert
               Fragment ends: MluI and SphI
               Fragment size: 505
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    320..614
                     /label=5'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    615..1528
                     /label=Psi+
                     /note="/vntifkey=""21"""
     misc_feature    1529..1906
                     /label=Pol\region\with\splice\acceptor
                     /note="/vntifkey=""21"""
     misc_feature    1970..2458
                     /label=CD90.2 optimized
                     /label=CD90.2\optimized
                     /note="/vntifkey=""21"""
     misc_feature    1970..1990
                     /label=Fwd
     misc_feature    2435..2458
                     /label=Rev
     misc_feature    2486..2522
                     /label='Fwd
     misc_feature    2587..2711
                     /label='Cd247AmiR'
     misc_feature    2592..2711
                     /label=shRNAmir core'
     misc_feature    2789..2828
                     /label=Rev'
     misc_feature    2885..2987
                     /label=Env\region
                     /note="/vntifkey=""21"""
     misc_feature    3024..3614
                     /label=3'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    complement(4667..5529)
                     /label=AmpR
                     /note="/vntifkey=""21"""
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
      541 ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct
      601 cgggggtctt tcatttggag gttccaccga gatttggaga ccccagccca gggaccaccg
      661 acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg
      721 cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc
      781 tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg
      841 agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga
      901 tcgttttgga ctctttggtg caccccccta ataggaggga tatgtggttc tggtaggaga
      961 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag
     1021 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1081 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1141 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1201 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1261 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1321 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1381 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1441 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1501 cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc
     1561 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     1621 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     1681 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     1741 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     1801 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     1861 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccggatc tagctagtta
     1921 attaactccg tggatccggt cgtgcggccg cacggaaacg cgtgccacca tgaaccccgc
     1981 catctctgtg gccctgctgc tgtctgtgct gcaggtgtcc agaggccaga aagtgaccag
     2041 cctgaccgcc tgcctcgtga accagaacct gagactggac tgcagacacg agaacaacac
     2101 caaggacaac agcatccagc acgagttctc cctgacccgc gagaagagaa aacacgtgct
     2161 gagcggcacc ctgggcatcc ccgagcacac ctacagatcc agagtgaccc tgagcaacca
     2221 gccctacatc aaggtgctga ccctggccaa cttcaccaca aaggacgagg gcgactactt
     2281 ctgcgaactg caggtgtcag gcgccaaccc catgagcagc aacaagagca tcagcgtgta
     2341 cagagacaag ctcgtgaagt gcggcggcat cagcctgctg gtgcagaaca cctcttggat
     2401 gctgttgctg ctgctgagcc tgagcctgct gcaggccctg gacttcatct ctctgtaggc
     2461 atgccgcgac cctgcaggtt tctgaagctt tgtttgaatg aggcttcagt actttacaga
     2521 atcgttgcct gcacatcttg gaaacacttg ctgggattac ttcttcaggt taacccaaca
     2581 gaaggctcga gaaggtatat tgctgttgac agtgagcgcc ctcttcatct acggagtcat
     2641 tagtgaagcc acagatgtaa tgactccgta gatgaagagg atgcctactg cctcggactt
     2701 caaggggcta gaattcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa
     2761 taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat
     2821 cactttatcg attgcattgg gtcgaccggg ccgccagcac agtggtcgat cgaggggggg
     2881 cccgcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac
     2941 tcaacaatat caccagctga agcctataga gtacgagcca tagatagaat aaaagatttt
     3001 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     3061 ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga
     3121 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
     3181 gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga
     3241 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     3301 ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     3361 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     3421 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
     3481 ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     3541 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     3601 gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
     3661 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
     3721 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3781 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3841 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3901 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3961 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4021 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4081 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4141 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4201 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4261 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4321 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4381 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4441 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4501 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4561 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg
     4621 gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4681 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4741 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4801 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4861 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4921 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4981 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5041 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5101 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5161 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5221 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5281 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5341 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5401 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5461 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5521 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5581 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
//
