LOCUS       Exported                6249 bp ds-DNA     circular SYN 09-NOV-2020
DEFINITION  Ligation of #S265 pMY-CD90.2-RLuc_shRNAmiR* into #83 
            pMY-Ly6G-P2A-PuroHA-Mir30*.
ACCESSION   .
VERSION     .
KEYWORDS    #S297 pMY-Ly6G-P2A-PuroHA-RLuc_shRNAmiR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 9, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
COMMENT     Vector molecule:  #83 pMY-Ly6G-P2A-PuroHA-Mir30*
               Fragment ends: EcoRI and HindIII
               Fragment size: 6023
            Insert molecule:  #S265 pMY-CD90.2-RLuc_shRNAmiR*
               Fragment ends: HindIII and EcoRI
               Fragment size: 226
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    320..614
                     /gene="5'\LTR"
                     /label=5' LTR
                     /label=5'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    615..1528
                     /label=Psi+
                     /note="/vntifkey=""21"""
     misc_feature    1529..1906
                     /gene="Pol\region\with\splice\acceptor"
                     /label=Splice acceptor
                     /label=Pol\region\with\splice\acceptor
                     /note="/vntifkey=""21"""
     misc_feature    1970..2371
                     /label=Ly6Gopt
     misc_feature    1970..1995
                     /label=Ly6G Opt Fwd
     misc_feature    2350..2371
                     /label=Ly6G Opt Rev
     misc_feature    2387..2443
                     /label=P2A
     misc_feature    2444..3094
                     /label=PuroHA
     misc_feature    2444..3040
                     /label=PuroR
     misc_feature    2444..2464
                     /label=Fwd
     misc_feature    3024..3040
                     /label=Rev
     misc_feature    3050..3076
                     /label=HA tag
     misc_feature    3095..3131
                     /label='Fwd
     misc_feature    3196..3209
                     /label='miRE-Xho-Fwd
                     /label=miRE-Xho-Fwd
                     /note="/vntifkey=""21"""
     misc_feature    3210..3306
                     /label=Luc_miR
     misc_feature    3210..3227
                     /label=Non-variable
     misc_feature    3250..3268
                     /label=Loop
     misc_feature    3291..3306
                     /label=Non-variable
     misc_feature    complement(3307..3320)
                     /label='miRE-Eco-Rev
                     /label=miRE-Eco-Rev
                     /note="/vntifkey=""21"""
     misc_feature    3321..3435
                     /label='miR30
     misc_feature    3398..3437
                     /label=Rev'
     misc_feature    3494..3596
                     /gene="Env\region"
                     /label=Env region
                     /label=Env\region
                     /note="/vntifkey=""21"""
     misc_feature    3633..4223
                     /gene="3'\LTR"
                     /label=3' LTR
                     /label=3'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    complement(5276..6138)
                     /label=AmpR
                     /note="/vntifkey=""21"""
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
      541 ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct
      601 cgggggtctt tcatttggag gttccaccga gatttggaga ccccagccca gggaccaccg
      661 acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg
      721 cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc
      781 tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg
      841 agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga
      901 tcgttttgga ctctttggtg caccccccta ataggaggga tatgtggttc tggtaggaga
      961 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag
     1021 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1081 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1141 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1201 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1261 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1321 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1381 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1441 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1501 cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc
     1561 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     1621 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     1681 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     1741 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     1801 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     1861 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccggatc tagctagtta
     1921 attaactccg tggatccggt cgtgcggccg cacggaaacg cgtgccacca tggacacctg
     1981 tcatatcgcc aagagctgcg tgctgatcct gctggtggtt ctgctgtgtg ccgagcgagc
     2041 acagggactg gagtgctaca actgtatcgg cgtgccacct gagacaagct gcaacaccac
     2101 cacctgtcct ttcagcgacg gcttctgtgt ggccctggaa atcgaagtga tcgtggacag
     2161 ccaccgcagc aaagtgaagt ccaacctgtg cctgcctatc tgccccacca cactggacaa
     2221 caccgagatc acaggcaacg ccgtgaacgt gaaaacctac tgctgcaaag aggacctctg
     2281 caacgccgct gttccaacag gcggaagctc ttggacaatg gctggcgtgc tgctgttcag
     2341 cctggtgtct gttctgctgc agaccttcct gggcagcggc gtcgacgcca caaacttcag
     2401 cctgctgaga caggctggcg acgtggaaga gaatcctgga cctatgaccg agtacaagcc
     2461 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     2521 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     2581 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     2641 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     2701 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     2761 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     2821 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     2881 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     2941 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     3001 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc ggcagcggct acccttacga
     3061 tgtgcctgat tacgcctgac ctgcaggttt ctgaagcttt gtttgaatga ggcttcagta
     3121 ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact tcttcaggtt
     3181 aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcag gaattataat
     3241 gcttatctat agtgaagcca cagatgtata gataagcatt ataattccta tgcctactgc
     3301 ctcggacttc aaggggctag aattcaaggg gctactttag gagcaattat cttgtttact
     3361 aaaactgaat accttgctat ctctttgata catttttaca aagctgaatt aaaatggtat
     3421 aaattaaatc actttatcga ttgcattggg tcgaccgggc cgccagcaca gtggtcgatc
     3481 gagggggggc ccgcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct
     3541 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agatagaata
     3601 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     3661 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
     3721 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
     3781 tggtaagcag ttcctgcccc ggctcagggc caagaacagt tggaacagca gaatatgggc
     3841 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     3901 cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     3961 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     4021 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc
     4081 gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt gtatccaata aaccctcttg
     4141 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     4201 tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc
     4261 ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga
     4321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4681 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4921 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5161 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5221 ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5581 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5881 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6241 gcacatttc
//
