LOCUS       Exported                5987 bp ds-DNA     circular SYN 09-NOV-2020
DEFINITION  #D120 pMY-Lyz-mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    #D120 pMY-Lyz-mTurquoise2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 9, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
COMMENT     Vector molecule:  #D106 pMY-Lyz2-mCherry*
               Fragment ends: HindIII and SalI
               Fragment size: 5252
            Insert molecule:  mTurquoise2 cds*
               Fragment ends: SalI and HindIII
               Fragment size: 735
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    22..614
                     /gene="5'\LTR"
                     /label=5' LTR
                     /label=5'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    615..1528
                     /label=Psi+
                     /note="/vntifkey=""21"""
     misc_feature    1529..1906
                     /gene="Pol\region\with\splice\acceptor"
                     /label=splice acceptor
                     /label=Pol\region\with\splice\acceptor
                     /note="/vntifkey=""21"""
     misc_feature    1970..2413
                     /label=Lyz2
     misc_feature    2438..3157
                     /label=mTurquoise2
     misc_feature    3232..3334
                     /gene="Env\region"
                     /label=Env region
                     /label=Env\region
                     /note="/vntifkey=""21"""
     misc_feature    3371..3961
                     /gene="3'\LTR"
                     /label=3' LTR
                     /label=3'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    4080..4942
                     /label=pUC ORI
     misc_feature    complement(5014..5874)
                     /gene="AmpR"
                     /label=AmpR
                     /note="/vntifkey=""21"""
     misc_feature    complement(5875..5944)
                     /label=Amp promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
      541 ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct
      601 cgggggtctt tcatttggag gttccaccga gatttggaga ccccagccca gggaccaccg
      661 acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg
      721 cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc
      781 tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg
      841 agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga
      901 tcgttttgga ctctttggtg caccccccta ataggaggga tatgtggttc tggtaggaga
      961 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag
     1021 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1081 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1141 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1201 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1261 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1321 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1381 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1441 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1501 cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc
     1561 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     1621 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     1681 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     1741 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     1801 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     1861 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccggatc tagctagtta
     1921 attaactccg tggatccggt cgtgcggccg cacggaaacg cgtgccacca tgaagactct
     1981 cctgactctg ggactcctcc tgctttctgt cactgctcag gccaaggtct atgaacgttg
     2041 tgagtttgcc agaactctga aaaggaatgg aatggctggc tactatggag tcagcctggc
     2101 cgactgggtg tgtttagctc agcacgagag caattataac acacgagcta caaactacaa
     2161 ccgtggagac caaagcactg actatgggat atttcagatc aatagccgat actggtgtaa
     2221 tgatggcaaa accccaagag ctgtgaatgc ctgtgggatc aattgcagtg ctctgctgca
     2281 ggatgacatc actgcagcca tacaatgtgc aaagagggtg gtgagagatc cccaaggcat
     2341 tcgagcatgg gtggcatggc gagcacactg tcaaaaccga gatctgtccc agtatattcg
     2401 gaactgcgga gtcggatctg gcgtcgacgg atctggcatg gtgagcaagg gcgaggagct
     2461 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2521 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2581 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgtcctgggg
     2641 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2701 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2761 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2821 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actactttag
     2881 cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     2941 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     3001 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     3061 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3121 cgggatcact ctcggcatgg acgagctgta caagtaaaag cttgagtaca tcgattgcat
     3181 tgggtcgatc gaccgggccg ccagcacagt ggtcgatcga gggggggccc gcgattagtc
     3241 caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac
     3301 cagctgaagc ctatagagta cgagccatag atagaataaa agattttatt tagtctccag
     3361 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     3421 ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa
     3481 cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     3541 ctcagggcca agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag
     3601 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca
     3661 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
     3721 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     3781 ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg
     3841 cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
     3901 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
     3961 acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc
     4021 catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4081 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4681 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4921 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccggcc gcaaatcaat
     4981 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5041 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5101 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5161 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5221 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5281 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5341 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5401 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5461 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5521 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5581 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5641 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5701 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5761 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5821 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5881 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5941 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttc
//
