LOCUS       Exported                5377 bp ds-DNA     circular SYN 09-NOV-2020
DEFINITION  #78 pMY-P2A-CD90.2.
ACCESSION   .
VERSION     .
KEYWORDS    #78 pMY-P2A-CD90.2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 9, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
COMMENT     Vector molecule:  #72 bam hind lin
               Fragment ends: HindIII and BamHI
               Fragment size: 4761
            Insert molecule:  CD90.2 insert
               Fragment ends: BamHI and HindIII
               Fragment size: 616
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    22..614
                     /gene="5'\LTR"
                     /label=5' LTR
                     /label=5'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    615..1528
                     /label=Psi+
                     /note="/vntifkey=""21"""
     misc_feature    1529..1906
                     /gene="Pol\region\with\splice\acceptor"
                     /label=splice acceptor
                     /label=Pol\region\with\splice\acceptor
                     /note="/vntifkey=""21"""
     misc_feature    1988..2044
                     /label=P2A
     misc_feature    2045..2534
                     /label=CD90.2
     misc_feature    2045..2065
                     /label=CD90.2 Fwd
     misc_feature    2622..2724
                     /gene="Env\region"
                     /label=Env region
                     /label=Env\region
                     /note="/vntifkey=""21"""
     misc_feature    2761..3351
                     /gene="3'\LTR"
                     /label=3' LTR
                     /label=3'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    3470..4332
                     /label=pUC ORI
     misc_feature    complement(4404..5264)
                     /gene="AmpR"
                     /label=AmpR
                     /note="/vntifkey=""21"""
     misc_feature    complement(5265..5334)
                     /label=Amp promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
      541 ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct
      601 cgggggtctt tcatttggag gttccaccga gatttggaga ccccagccca gggaccaccg
      661 acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg
      721 cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc
      781 tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg
      841 agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga
      901 tcgttttgga ctctttggtg caccccccta ataggaggga tatgtggttc tggtaggaga
      961 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag
     1021 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1081 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1141 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1201 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1261 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1321 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1381 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1441 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1501 cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc
     1561 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     1621 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     1681 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     1741 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     1801 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     1861 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccggatc tagctagtta
     1921 attaactccg tggatccggt cgtgcggccg cacggaaacg cgtggcctgg catgccgcga
     1981 cgtcgacgcc acaaacttca gcctgctgag acaggctggc gacgtggaag agaatcctgg
     2041 acctatgaac cccgccatct ctgtggccct gctgctgtct gtgctgcagg tgtccagagg
     2101 ccagaaagtg accagcctga ccgcctgcct cgtgaaccag aacctgagac tggactgcag
     2161 acacgagaac aacaccaagg acaacagcat ccagcacgag ttctccctga cccgcgagaa
     2221 gagaaaacac gtgctgagcg gcaccctggg catccccgag cacacctaca gatccagagt
     2281 gaccctgagc aaccagccct acatcaaggt gctgaccctg gccaacttca ccacaaagga
     2341 cgagggcgac tacttctgcg aactgcaggt gtcaggcgcc aaccccatga gcagcaacaa
     2401 gagcatcagc gtgtacagag acaagctcgt gaagtgcggc ggcatcagcc tgctggtgca
     2461 gaacacctct tggatgctgt tgctgctgct gagcctgagc ctgctgcagg ccctggactt
     2521 catctctctg tagcctgcag gtttctgaag cttgagtaca tcgattgcat tgggtcgatc
     2581 gaccgggccg ccagcacagt ggtcgatcga gggggggccc gcgattagtc caatttgtta
     2641 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     2701 ctatagagta cgagccatag atagaataaa agattttatt tagtctccag aaaaaggggg
     2761 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     2821 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     2881 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     2941 agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     3001 cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta
     3061 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     3121 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     3181 taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg
     3241 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     3301 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca
     3361 tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca
     3421 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3481 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3541 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3601 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3661 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3721 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3781 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3841 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3901 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3961 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4021 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4081 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4141 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4201 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4261 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4321 atcaaaaagg atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata
     4381 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4441 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4501 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4561 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4621 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4681 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4741 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4801 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4861 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4921 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4981 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5041 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5101 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5161 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5221 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5281 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5341 tagaaaaata aacaaatagg ggttccgcgc acatttc
//
