LOCUS       Exported                5344 bp ds-DNA     circular SYN 09-NOV-2020
DEFINITION  Ligation of P2A-BlastR-DYK-Sal-Sbf cloning* into #74 
            pMY-P2A-PuroR-HA*.
ACCESSION   .
VERSION     .
KEYWORDS    #88 pMY-P2A-BlastR-DYK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 9, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
COMMENT     Vector molecule:  #74 pMY-P2A-PuroR-HA*
               Fragment ends: SbfI and SalI
               Fragment size: 4820
            Insert molecule:  P2A-BlastR-DYK-Sal-Sbf cloning*
               Fragment ends: SalI and SbfI
               Fragment size: 524
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    22..614
                     /gene="5'\LTR"
                     /label=5' LTR
                     /label=5'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    615..1528
                     /label=Psi+
                     /note="/vntifkey=""21"""
     misc_feature    1529..1906
                     /gene="Pol\region\with\splice\acceptor"
                     /label=splice acceptor
                     /label=Pol\region\with\splice\acceptor
                     /note="/vntifkey=""21"""
     misc_feature    1988..2044
                     /label=P2A
     misc_feature    2045..2497
                     /label=BlastR-DYK
     misc_feature    2045..2094
                     /label=Fwd
     misc_feature    2427..2464
                     /label=Rev
     misc_feature    2589..2691
                     /gene="Env\region"
                     /label=Env region
                     /label=Env\region
                     /note="/vntifkey=""21"""
     misc_feature    2728..3318
                     /gene="3'\LTR"
                     /label=3' LTR
                     /label=3'\LTR
                     /note="/vntifkey=""21"""
     misc_feature    3437..4299
                     /label=pUC ORI
     misc_feature    complement(4371..5231)
                     /gene="AmpR"
                     /label=AmpR
                     /note="/vntifkey=""21"""
     misc_feature    complement(5232..5301)
                     /label=Amp promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
      541 ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct
      601 cgggggtctt tcatttggag gttccaccga gatttggaga ccccagccca gggaccaccg
      661 acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg
      721 cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc
      781 tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg
      841 agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga
      901 tcgttttgga ctctttggtg caccccccta ataggaggga tatgtggttc tggtaggaga
      961 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag
     1021 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1081 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1141 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1201 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1261 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1321 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1381 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1441 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1501 cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc
     1561 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     1621 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     1681 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     1741 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     1801 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     1861 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccggatc tagctagtta
     1921 attaactccg tggatccggt cgtgcggccg cacggaaacg cgtggcctgg catgccgcga
     1981 cgtcgacgcc acaaacttca gcctgctgag acaggctggc gacgtggaag agaatcctgg
     2041 acctatgaaa acatttaaca tttctcaaca agatctagaa ttagtagaag tagcgacaga
     2101 gaagattaca atgctttatg aggataataa acatcatgtg ggagcggcaa ttcgtacgaa
     2161 aacaggagaa atcatttcgg cagtacatat tgaagcgtat ataggacgag taactgtttg
     2221 tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga caaaaggatt ttgacacgat
     2281 tgtagctgtt agacaccctt attctgacga agtagataga agtattcgag tggtaagtcc
     2341 ttgtggtatg tgtagggagt tgatttcaga ctatgcacca gattgttttg tgttaataga
     2401 aatgaatggc aagttagtca aaactacgat tgaagaactc attccactca aatatacccg
     2461 aaatggcagc ggcgactaca aggacgacga cgacaaatga cctgcaggtt tctgaagctt
     2521 gagtacatcg attgcattgg gtcgatcgac cgggccgcca gcacagtggt cgatcgaggg
     2581 ggggcccgcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
     2641 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata gaataaaaga
     2701 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     2761 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt
     2821 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta
     2881 agcagttcct gccccggctc agggccaaga acagttggaa cagcagaata tgggccaaac
     2941 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     3001 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     3061 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     3121 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     3181 agtcctccga tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     3241 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     3301 gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag
     3361 tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3421 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3481 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3541 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3601 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3661 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3721 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3781 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3841 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3901 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3961 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4021 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4081 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4141 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4201 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4261 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg
     4321 gccggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4381 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4441 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4501 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4561 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4621 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4681 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4741 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4801 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4861 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4921 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4981 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5041 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5101 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5161 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5221 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5281 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5341 tttc
//
