LOCUS Slingshot2_gRNA_ 2813 bp ds-DNA circular 22-OCT-2020 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 73..313 /locus_tag="U6 promoter" /label="U6 promoter" /ApEinfo_label="U6 promoter" /ApEinfo_fwdcolor="#ff80c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 342..417 /locus_tag="gRNA scafold" /label="gRNA scafold" /ApEinfo_label="gRNA scafold" /ApEinfo_fwdcolor="#c0c0c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 342..356 /locus_tag="Insert position 2" /label="Insert position 2" /ApEinfo_label="Insert position 2" /ApEinfo_fwdcolor="#0000a0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 182..210 /locus_tag="gRNA backbone seq-F" /label="gRNA backbone seq-F" /ApEinfo_label="gRNA backbone seq-F" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 307..321 /locus_tag="Insert position1" /label="Insert position1" /ApEinfo_label="Insert position1" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 61 ccacctgacg tcGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 121 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 181 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 241 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 301 TTGTGGAAAG GACGAAACAC CgGCAGTAGC ACTTGTACCA TGTTTTAGAG CTAGAAATAG 361 CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT 421 TTTTCTAGAC CCAGCTTTCT TGTACAAAGT TGGCATTAga tccgctgcat taatgaatcg 481 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 541 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 601 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccaga 661 attcGAGCTC ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt 721 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 841 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc CAGCTGcatt 901 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 961 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 1021 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 1081 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 1141 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 1201 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 1261 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 1321 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 1381 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 1441 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 1501 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 1561 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 1621 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 1681 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1741 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 1801 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1861 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 1921 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 1981 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 2041 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 2101 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 2161 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 2221 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 2281 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 2341 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 2401 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 2461 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 2521 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 2581 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 2641 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 2701 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 2761 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac ata //