LOCUS Nup133_mEGFP(-8b) 8645 bp ds-DNA linear 04-SEP-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature 681..701 /label="Gateway recomb1" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind complement(5071..5090) /label="T7" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS 6723..7382 /label="AmpR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 4297..4998 /label="GFP" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_recomb complement(7487..7520) /label="LoxP" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_recomb 681..701 /label="attB1" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 promoter 305..508 /label="CMV promoter" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 promoter 661..679 /label="SP6 promoter" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 659..678 /label="SP6" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 838..4296 /label="Nup133" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 rep_origin 5799..6105 /label="F1 ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 promoter 1..527 /label="CMV promoter" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc promoter 1..304 /label="CMV enhancer" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind complement(5101..5118) /label="M13-fwd" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_recomb complement(5049..5069) /label="attB2" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(5049..5069) /label="Gateway Recomb2" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e rep_origin 5782..6237 /label="M13 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS 5712..5780 /label="LacZ alpha" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc polyA_signal complement(5461..5652) /label="SV40 late polyA" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg 541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct 601 agcctaggcc gcggagcgga taacaatttc acacaggaaa cagctatgac cattaggcct 661 atttaggtga cactatagaa caagtttgta caaaaaagca ggctggtacc ggtccggaat 721 tcccgggata tcGTCGACCC ACGCGTCCGC CCACGCGTCC Gttaagttcc gcgcgcaggc 781 caggctgcaa cctgacggcc agatccctcg ctgtcctagt cgctgctcct tggagtcatg 841 ttcccagccg ccccttctcc gcggaccccg ggtaccgggt cccgaagggg cccgctggcc 901 ggactcgggc ccggctccac gccccggacg gctagcagga agggtctgcc cctggggtct 961 gcagtcagct ccccagtgct cttctcgccg gtcggccggc gtagctcgct aagctcgcgg 1021 ggaacaccaa cacgaatgtt cccacaccac tccataactg agtctgtgaa ctatgatgtg 1081 aaaacgtttg gatcttctct tcctgttaaa gtcatggaag ccctaacatt ggctgaagtc 1141 gatgaccagc tgaccattaa catagatgaa ggtggatggg cttgtctggt gtgcaaagag 1201 aagctcatta tttggaagat tgctctgtca cctattacta agttatccgt ttgcaaagaa 1261 cttcagctgc cacctagtga tttccactgg agtgccgact tagtggctct ttcttactct 1321 tctccctcag gtgaagcaca ttctactcag gctgttgctg tcatggttgc caccagagaa 1381 ggatctatcc gctattggcc aagccttgct ggtgaagata cctacacaga ggcttttgta 1441 gattcgggag gtgataagac ttacagtttc ctaacagcag tgcagggagg aagttttatt 1501 ttgtcttcat caggaagcca actaattcgg ttgatacctg agagctcagg aaagattcat 1561 cagcatatcc tgcctcaggg gcaaggcatg ctttcaggaa ttggtcgaaa agtttcttct 1621 ctttttggaa ttttatctcc tagtagtgat ctcacacttt caagtgttct ctgggataga 1681 gagagatcaa gcttttatag cctgacgagt tcaaacatca gtaaatggga attagatgat 1741 tcttcagaaa agcatgcata cagttgggat ataaatagag ccctgaagga aaacattacc 1801 gatgctattt ggggatctga aagtaactat gaagctatta aagaaggagt caacattcga 1861 tatttggact ttaagcaaaa ctgtgatggg ctggtgattt tggcagcagc atggcactca 1921 gcagacaatc catgtctcat ctattactct ctgataacaa tagaagataa tggttgccaa 1981 atgtcagatg cagttactgt agaagtcact caatataatc caccttttca gtctgaagac 2041 ctgattttgt gtcagttgac ggtcccaaac ttttcaaacc agactgccta tctgtataac 2101 gaaagtgctg tctatgtgtg ctccacagga actgggaaat tttctcttcc ccaggagaaa 2161 attgtcttta atgcacaagg agatagtgtt ttaggtgctg gtgcctgtgg tggtgttcct 2221 atcatttttt ctagaaacag tggactggtg tctattactt caagggaaaa tgtgtctata 2281 ttggcagaag acttggaagg gtctttagca tcttcagttg ctggaccaaa cagtgagagt 2341 atgatttttg agaccactac aaagaatgaa actatagccc aggaagataa aatcaagttg 2401 ctgaaagctg cctttctgca atactgcaga aaagatttag gtcatgctca aatggtggtt 2461 gatgagctct tttcctctca ctctgatttg gattctgatt ctgaactaga cagggcagtt 2521 acccaaatca gtgtagacct gatggatgac tacccagcat ctgacccacg gtgggctgag 2581 tctgtccctg aggaagcacc tgggttcagc aatacgtcac tgattatcct tcaccagcta 2641 gaagacaaga tgaaagctca ctcttttctt atggacttta ttcatcaagt tggcttattt 2701 ggacgtctag gcagttttcc agttagaggg acaccgatgg ccactcgact gttgctctgt 2761 gagcatgccg aaaagctgtc agccgccatt gttctcaaga accaccactc ccggctttct 2821 gaccttgtca acacagccat attgattgct ttgaacaaga gggagtatga aatcccatcc 2881 aacctgactc ctgcagatgt ctttttcagg gaggtatccc aagtagatac catctgtgag 2941 tgcttactgg agcatgagga gcaagtcttg agggatgcac ctatggattc cattgaatgg 3001 gctgaagtgg tgatcaatgt gaacaatatt ctcaaggata tgctgcaggc tgctagtcat 3061 tatcgccaaa atagaaactc tttgtataga agagaagaat cactagaaaa agaacctgaa 3121 tatgttccat ggacggcaac aagtggtcct ggtggcatcc gaacggtaat aatacgccag 3181 catgagattg tcctgaaggt ggcttatcca caggcagaca gcaacctccg aaacatcgtg 3241 accgagcagc tggtagccct gatcgattgc ttcctggatg gttatgtttc tcagcttaag 3301 tctgtggata aatccagtaa tcgggaaaga tatgacaatc tggagatgga atacctacag 3361 aaaagatcag atctcttatc tcctcttctt tcactaggcc agtacctgtg ggctgcttct 3421 ctagcagaga aatactgtga ctttgatata ttggtacaaa tgtgtgagca gactgacaac 3481 cagagccgac tccagcgcta catgacccag tttgctgatc agaatttttc agactttctc 3541 ttccgttggt atctggagaa aggaaagcga ggcaaattat tatctcagcc catttctcag 3601 catggacagt tggcaaattt tttgcaagct catgaacatc tcagctggtt acatgaaatt 3661 aatagccaag aattagaaaa ggctcatgca acacttctgg gtttggcaaa tatggaaact 3721 cgttactttg caaagaagaa aacccttctt ggcttgagta aattggctgc attagcttca 3781 gacttttcag aggatatgct acaagaaaaa attgaagaaa tggctgagca ggagcgcttt 3841 ctactgcatc aggagaccct acctgaacag ctgctggcgg agaaacagct aaatctcagt 3901 gcgatgccag tattgactgc accacaactc attggtctat atatctgtga agaaaataga 3961 agagctaatg aatatgattt caagaaagct ttggacttgt tggaatatat tgatgaggaa 4021 gaagatataa atataaatga tctaaaactg gaaatccttt gcaaagctct tcagagagat 4081 aactggtcca gttctgatgg caaagatgat ccaattgaag tatctaaaga cagtatattt 4141 gtgaagatct tacagaaact tttaaaagat ggcattcagc tcagtgagta cttaccggag 4201 gtgaaagacc tgctacaagc ggatcagctt ggaagcttaa agtccaatcc ttacttcgag 4261 tttgttttga aagcaaatta tgaatattat gttCAGGAGG AGCTGTTCAC CGGGGTGGTG 4321 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG 4381 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG 4441 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC 4501 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC 4561 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG 4621 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG 4681 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC 4741 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG 4801 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC 4861 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC 4921 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC 4981 ATGGACGAGC TGTACAAGta aGCGGCCGCt ctagagtatc cctcgagggg cccaagctta 5041 cgcgtaccca gctttcttgt acaaagtggt ccctatagtg agtcgtatta taagctaggc 5101 actggccgtc gttttacaac gtcgtgactg ggaaaactgc tagcttggga tctttgtgaa 5161 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc 5221 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctagctgcat atgcttgctg 5281 cttgagagtt ttgcttactg agtatgattt atgaaaatat tatacacagg agctagtgat 5341 tctaattgtt tgtgtatttt agattcacag tcccaaggct catttcaggc ccctcagtcc 5401 tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt tacttgcttt 5461 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 5521 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5581 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 5641 ttatcatgtc tggatcgatc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 5701 tgcgtattgg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 5761 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 5821 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 5881 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 5941 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 6001 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 6061 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 6121 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 6181 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag 6241 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 6301 caaatatgta tccgctcatg ccaggtcttg gactggtgag aacggcttgc tcggcagctt 6361 cgatgtgtgc tggagggaga ataaaggtct aagatgtgcg atagagggaa gtcgcattga 6421 attatgtgct gtgtagggat cgctggtatc aaatatgtgt gcccacccct ggcatgagac 6481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 6541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 6601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 6661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 6721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 6781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 6841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 6901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 6961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 7021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 7081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 7141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 7201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 7261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 7321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 7381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 7441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgccataa cttcgtataa 7501 tgtatgctat acgaagttat ggcatgacca aaatccctta acgtgagttt tcgttccact 7561 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 7621 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 7681 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 7741 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 7801 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 7861 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 7921 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 7981 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 8041 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 8101 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 8161 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 8221 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 8281 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 8341 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 8401 gttggccgat tcattaatgc agagcttgca attcgcgcgt ttttcaatat tattgaagca 8461 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 8521 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 8581 ttatcatgac attaacctat aaaaataggc gtagtacgag gccctttcac tcattagatg 8641 catgt //